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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter baumannii 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP481980AWM
CSB-EP481980AWM
CSB-BP481980AWM
CSB-MP481980AWM
CSB-EP481980AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Chaperone protein hscA homolog (hscA), partial CSB-YP481981AWM
CSB-EP481981AWM
CSB-BP481981AWM
CSB-MP481981AWM
CSB-EP481981AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Diaminopimelate epimerase (dapF) CSB-YP481982AWM
CSB-EP481982AWM
CSB-BP481982AWM
CSB-MP481982AWM
CSB-EP481982AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0042 nucleotide-binding protein AB57_0691 (AB57_0691) CSB-YP481983AWM
CSB-EP481983AWM
CSB-BP481983AWM
CSB-MP481983AWM
CSB-EP481983AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Histidinol-phosphate aminotransferase (hisC) CSB-YP481984AWM
CSB-EP481984AWM
CSB-BP481984AWM
CSB-MP481984AWM
CSB-EP481984AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii NADH-quinone oxidoreductase subunit N (nuoN), partial CSB-YP481985AWM
CSB-EP481985AWM
CSB-BP481985AWM
CSB-MP481985AWM
CSB-EP481985AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP481986AWM
CSB-EP481986AWM
CSB-BP481986AWM
CSB-MP481986AWM
CSB-EP481986AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Putative arginyl-tRNA--protein transferase (ate) CSB-YP481987AWM
CSB-EP481987AWM
CSB-BP481987AWM
CSB-MP481987AWM
CSB-EP481987AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Probable malate:quinone oxidoreductase (mqo), partial CSB-YP481988AWM
CSB-EP481988AWM
CSB-BP481988AWM
CSB-MP481988AWM
CSB-EP481988AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP481989AWM
CSB-EP481989AWM
CSB-BP481989AWM
CSB-MP481989AWM
CSB-EP481989AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Urease subunit gamma (ureA) CSB-YP481990AWM
CSB-EP481990AWM
CSB-BP481990AWM
CSB-MP481990AWM
CSB-EP481990AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP481991AWM
CSB-EP481991AWM
CSB-BP481991AWM
CSB-MP481991AWM
CSB-EP481991AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP481992AWM
CSB-EP481992AWM
CSB-BP481992AWM
CSB-MP481992AWM
CSB-EP481992AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii UPF0758 protein AB57_3420 (AB57_3420) CSB-YP481993AWM
CSB-EP481993AWM
CSB-BP481993AWM
CSB-MP481993AWM
CSB-EP481993AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Xanthine phosphoribosyltransferase (xpt) CSB-YP481994AWM
CSB-EP481994AWM
CSB-BP481994AWM
CSB-MP481994AWM
CSB-EP481994AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Carbon storage regulator homolog (csrA) CSB-YP481995AWM
CSB-EP481995AWM
CSB-BP481995AWM
CSB-MP481995AWM
CSB-EP481995AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP481996AWM
CSB-EP481996AWM
CSB-BP481996AWM
CSB-MP481996AWM
CSB-EP481996AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L15 (rplO) CSB-YP481997AWM
CSB-EP481997AWM
CSB-BP481997AWM
CSB-MP481997AWM
CSB-EP481997AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L24 (rplX) CSB-YP481998AWM
CSB-EP481998AWM
CSB-BP481998AWM
CSB-MP481998AWM
CSB-EP481998AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii 50S ribosomal protein L29 (rpmC) CSB-YP481999AWM
CSB-EP481999AWM
CSB-BP481999AWM
CSB-MP481999AWM
CSB-EP481999AWM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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