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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana TATA box-binding protein-associated factor RNA polymerase I subunit B (MEE12), partial CSB-YP723069DOA
CSB-EP723069DOA
CSB-BP723069DOA
CSB-MP723069DOA
CSB-EP723069DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sudan ebolavirus Membrane-associated protein VP24 (VP24) CSB-YP723070SRE
CSB-EP723070SRE
CSB-BP723070SRE
CSB-MP723070SRE
CSB-EP723070SRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sudan ebolavirus Polymerase cofactor VP35 (VP35) CSB-YP723071SRE
CSB-EP723071SRE
CSB-BP723071SRE
CSB-MP723071SRE
CSB-EP723071SRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phodopus sungorus Melanopsin (OPN4), partial CSB-YP723072EUT1
CSB-EP723072EUT1
CSB-BP723072EUT1
CSB-MP723072EUT1
CSB-EP723072EUT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Getah virus Non-structural polyprotein, partial CSB-YP723073GBAM
CSB-EP723073GBAM
CSB-BP723073GBAM
CSB-MP723073GBAM
CSB-EP723073GBAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bos mutus grunniens NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP723074BPD1
CSB-EP723074BPD1
CSB-BP723074BPD1
CSB-MP723074BPD1
CSB-EP723074BPD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis Beta/delta-agatoxin-5 CSB-YP723075AEQ
CSB-EP723075AEQ
CSB-BP723075AEQ
CSB-MP723075AEQ
CSB-EP723075AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis Beta/delta-agatoxin-1 CSB-YP723076AEQ
CSB-EP723076AEQ
CSB-BP723076AEQ
CSB-MP723076AEQ
CSB-EP723076AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U6-agatoxin-Ao1a CSB-YP723077AEQ
CSB-EP723077AEQ
CSB-BP723077AEQ
CSB-MP723077AEQ
CSB-EP723077AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U2-agatoxin-Ao1k CSB-YP723078AEQ
CSB-EP723078AEQ
CSB-BP723078AEQ
CSB-MP723078AEQ
CSB-EP723078AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agelena orientalis U2-agatoxin-Ao1c CSB-YP723079AEQ
CSB-EP723079AEQ
CSB-BP723079AEQ
CSB-MP723079AEQ
CSB-EP723079AEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus Taste receptor type 2 member 19 (TAS2R19), partial CSB-YP723080EQU1
CSB-EP723080EQU1
CSB-BP723080EQU1
CSB-MP723080EQU1
CSB-EP723080EQU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative sterol 14-demethylase-like protein (CYP51G2), partial CSB-YP723081DOA
CSB-EP723081DOA
CSB-BP723081DOA
CSB-MP723081DOA
CSB-EP723081DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hydra vulgaris Periculin-1 CSB-YP723082HXN
CSB-EP723082HXN
CSB-BP723082HXN
CSB-MP723082HXN
CSB-EP723082HXN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Transmembrane channel-like protein 7 (Tmc7), partial CSB-YP723083CH
CSB-EP723083CH
CSB-BP723083CH
CSB-MP723083CH
CSB-EP723083CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loxosceles boneti Sphingomyelin phosphodiesterase D LbSicTox-alphaIB1a CSB-YP723084LJD
CSB-EP723084LJD
CSB-BP723084LJD
CSB-MP723084LJD
CSB-EP723084LJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermophilus citellus Glycogen synthase kinase-3 beta (GSK3B) CSB-YP723085SJE
CSB-EP723085SJE
CSB-BP723085SJE
CSB-MP723085SJE
CSB-EP723085SJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Parkinsonia aculeata Maturase K (matK) CSB-YP723086PGAF
CSB-EP723086PGAF
CSB-BP723086PGAF
CSB-MP723086PGAF
CSB-EP723086PGAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Albizzia julibrissin Maturase K (matK) CSB-YP723087AGK
CSB-EP723087AGK
CSB-BP723087AGK
CSB-MP723087AGK
CSB-EP723087AGK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Endoglucanase A (eglA), partial CSB-YP723088BRC
CSB-EP723088BRC
CSB-BP723088BRC
CSB-MP723088BRC
CSB-EP723088BRC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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