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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Rickettsia typhi Malate dehydrogenase (mdh) CSB-YP724311RNE
CSB-EP724311RNE
CSB-BP724311RNE
CSB-MP724311RNE
CSB-EP724311RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi NADH-quinone oxidoreductase subunit D (nuoD) CSB-YP724312RNE
CSB-EP724312RNE
CSB-BP724312RNE
CSB-MP724312RNE
CSB-EP724312RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Glutamate--tRNA ligase 1 (gltX1) CSB-YP724313RNE
CSB-EP724313RNE
CSB-BP724313RNE
CSB-MP724313RNE
CSB-EP724313RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Thymidylate synthase thyX (thyX) CSB-YP724314RNE
CSB-EP724314RNE
CSB-BP724314RNE
CSB-MP724314RNE
CSB-EP724314RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Ubiquinol-cytochrome c reductase iron-sulfur subunit (petA), partial CSB-YP724315RNE1
CSB-EP724315RNE1
CSB-BP724315RNE1
CSB-MP724315RNE1
CSB-EP724315RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi D-alanine--D-alanine ligase (ddl) CSB-YP724316RNE
CSB-EP724316RNE
CSB-BP724316RNE
CSB-MP724316RNE
CSB-EP724316RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi DNA topoisomerase 4 subunit B (parE), partial CSB-YP724317RNE
CSB-EP724317RNE
CSB-BP724317RNE
CSB-MP724317RNE
CSB-EP724317RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Glutaredoxin-1 (grxC1) CSB-YP724318RNE
CSB-EP724318RNE
CSB-BP724318RNE
CSB-MP724318RNE
CSB-EP724318RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Chaperone protein DnaJ (dnaJ) CSB-YP724319RNE
CSB-EP724319RNE
CSB-BP724319RNE
CSB-MP724319RNE
CSB-EP724319RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi N utilization substance protein B homolog (nusB) CSB-YP724320RNE
CSB-EP724320RNE
CSB-BP724320RNE
CSB-MP724320RNE
CSB-EP724320RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Probable cytosol aminopeptidase (pepA) CSB-YP724321RNE
CSB-EP724321RNE
CSB-BP724321RNE
CSB-MP724321RNE
CSB-EP724321RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Succinate dehydrogenase flavoprotein subunit (sdhA), partial CSB-YP724322RNE
CSB-EP724322RNE
CSB-BP724322RNE
CSB-MP724322RNE
CSB-EP724322RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi ATP synthase subunit a (atpB), partial CSB-YP724323RNE1
CSB-EP724323RNE1
CSB-BP724323RNE1
CSB-MP724323RNE1
CSB-EP724323RNE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi tRNA (Ile)-lysidine synthase (tilS) CSB-YP724324RNE
CSB-EP724324RNE
CSB-BP724324RNE
CSB-MP724324RNE
CSB-EP724324RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi ADP,ATP carrier protein 1 (tlcA), partial CSB-YP724325RNE
CSB-EP724325RNE
CSB-BP724325RNE
CSB-MP724325RNE
CSB-EP724325RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi DNA topoisomerase 4 subunit A (parC), partial CSB-YP724326RNE
CSB-EP724326RNE
CSB-BP724326RNE
CSB-MP724326RNE
CSB-EP724326RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 30S ribosomal protein S2 (rpsB) CSB-YP724327RNE
CSB-EP724327RNE
CSB-BP724327RNE
CSB-MP724327RNE
CSB-EP724327RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi 50S ribosomal protein L31 (rpmE) CSB-YP724328RNE
CSB-EP724328RNE
CSB-BP724328RNE
CSB-MP724328RNE
CSB-EP724328RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi Acetate kinase (ackA) CSB-YP724329RNE
CSB-EP724329RNE
CSB-BP724329RNE
CSB-MP724329RNE
CSB-EP724329RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia typhi UPF0079 ATP-binding protein RT0012 (RT0012) CSB-YP724330RNE
CSB-EP724330RNE
CSB-BP724330RNE
CSB-MP724330RNE
CSB-EP724330RNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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