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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mitochondrial import inner membrane translocase subunit TIM14 (dnj-21), partial CSB-YP733798CXX
CSB-EP733798CXX
CSB-BP733798CXX
CSB-MP733798CXX
CSB-EP733798CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Forkhead box protein C2 (Foxc2) CSB-YP733799MO
CSB-EP733799MO
CSB-BP733799MO
CSB-MP733799MO
CSB-EP733799MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Neurogenic locus notch homolog protein 3 (Notch3), partial CSB-YP733800MO
CSB-EP733800MO
CSB-BP733800MO
CSB-MP733800MO
CSB-EP733800MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ras and EF-hand domain-containing protein homolog (tag-312), partial CSB-YP733801CXX
CSB-EP733801CXX
CSB-BP733801CXX
CSB-MP733801CXX
CSB-EP733801CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mitochondrial import inner membrane translocase subunit Tim10 (tin-10) CSB-YP733802CXX
CSB-EP733802CXX
CSB-BP733802CXX
CSB-MP733802CXX
CSB-EP733802CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanine nucleotide-binding protein alpha-15 subunit (gpa-15) CSB-YP733803CXX
CSB-EP733803CXX
CSB-BP733803CXX
CSB-MP733803CXX
CSB-EP733803CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dopamine receptor 3 (dop-3), partial CSB-YP733804CXX1
CSB-EP733804CXX1
CSB-BP733804CXX1
CSB-MP733804CXX1
CSB-EP733804CXX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable citrate synthase, mitochondrial (cts-1), partial CSB-YP733805CXX
CSB-EP733805CXX
CSB-BP733805CXX
CSB-MP733805CXX
CSB-EP733805CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant IWS1-like protein (iws-1) CSB-YP733806CXX
CSB-EP733806CXX
CSB-BP733806CXX
CSB-MP733806CXX
CSB-EP733806CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent RNA helicase SUV3 homolog, mitochondrial (CBG06022), partial CSB-YP733807CXX
CSB-EP733807CXX
CSB-BP733807CXX
CSB-MP733807CXX
CSB-EP733807CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nascent polypeptide-associated complex subunit alpha (CBG05114) CSB-YP733808CXX
CSB-EP733808CXX
CSB-BP733808CXX
CSB-MP733808CXX
CSB-EP733808CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mediator of RNA polymerase II transcription subunit 1.1 (sop-3), partial CSB-YP733809CXX
CSB-EP733809CXX
CSB-BP733809CXX
CSB-MP733809CXX
CSB-EP733809CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leishmanolysin-like peptidase (CBG03556), partial CSB-YP733810CXX
CSB-EP733810CXX
CSB-BP733810CXX
CSB-MP733810CXX
CSB-EP733810CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Mimecan (Ogn) CSB-YP733811MO
CSB-EP733811MO
CSB-BP733811MO
CSB-MP733811MO
CSB-EP733811MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Serum paraoxonase/arylesterase 2 (Pon2) CSB-YP733812MO
CSB-EP733812MO
CSB-BP733812MO
CSB-MP733812MO
CSB-EP733812MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Receptor-type tyrosine-protein phosphatase R (Ptprr), partial CSB-YP733813MO
CSB-EP733813MO
CSB-BP733813MO
CSB-MP733813MO
CSB-EP733813MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho-related GTP-binding protein RhoC (Rhoc) CSB-YP733814MO
CSB-EP733814MO
CSB-BP733814MO
CSB-MP733814MO
CSB-EP733814MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse RalA-binding protein 1 (Ralbp1) CSB-YP733815MO
CSB-EP733815MO
CSB-BP733815MO
CSB-MP733815MO
CSB-EP733815MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse E3 ubiquitin-protein ligase TRIM21 (Trim21) CSB-YP733816MO
CSB-MP733816MO
CSB-EP733816MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Semaphorin-5A (Sema5a), partial CSB-YP733817MO
CSB-EP733817MO
CSB-BP733817MO
CSB-MP733817MO
CSB-EP733817MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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