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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Listeria monocytogenes serotype 4b Uncharacterized RNA methyltransferase LMOf2365_1776 (LMOf2365_1776) CSB-YP761776LAAD
CSB-EP761776LAAD
CSB-BP761776LAAD
CSB-MP761776LAAD
CSB-EP761776LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Uncharacterized RNA methyltransferase LMOf2365_1727 (LMOf2365_1727) CSB-YP761777LAAD
CSB-EP761777LAAD
CSB-BP761777LAAD
CSB-MP761777LAAD
CSB-EP761777LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b S-adenosylmethionine synthase (metK) CSB-YP761778LAAD
CSB-EP761778LAAD
CSB-BP761778LAAD
CSB-MP761778LAAD
CSB-EP761778LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP761779LAAD
CSB-EP761779LAAD
CSB-BP761779LAAD
CSB-MP761779LAAD
CSB-EP761779LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Septation ring formation regulator EzrA (ezrA), partial CSB-YP761780LAAD
CSB-EP761780LAAD
CSB-BP761780LAAD
CSB-MP761780LAAD
CSB-EP761780LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP761781LAAD
CSB-EP761781LAAD
CSB-BP761781LAAD
CSB-MP761781LAAD
CSB-EP761781LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Dephospho-CoA kinase (coaE) CSB-YP761782LAAD
CSB-EP761782LAAD
CSB-BP761782LAAD
CSB-MP761782LAAD
CSB-EP761782LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glutamyl-tRNA reductase (hemA) CSB-YP761783LAAD
CSB-EP761783LAAD
CSB-BP761783LAAD
CSB-MP761783LAAD
CSB-EP761783LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b GTPase obg (obg) CSB-YP761784LAAD
CSB-EP761784LAAD
CSB-BP761784LAAD
CSB-MP761784LAAD
CSB-EP761784LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Queuine tRNA-ribosyltransferase (tgt) CSB-YP761785LAAD
CSB-EP761785LAAD
CSB-BP761785LAAD
CSB-MP761785LAAD
CSB-EP761785LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b UPF0297 protein LMOf2365_1522 (LMOf2365_1522) CSB-YP761786LAAD
CSB-EP761786LAAD
CSB-BP761786LAAD
CSB-MP761786LAAD
CSB-EP761786LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Elongation factor 4 (lepA), partial CSB-YP761787LAAD
CSB-EP761787LAAD
CSB-BP761787LAAD
CSB-MP761787LAAD
CSB-EP761787LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Protein grpE (grpE) CSB-YP761788LAAD
CSB-EP761788LAAD
CSB-BP761788LAAD
CSB-MP761788LAAD
CSB-EP761788LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP761789LAAD
CSB-EP761789LAAD
CSB-BP761789LAAD
CSB-MP761789LAAD
CSB-EP761789LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Foldase protein prsA 1 (prsA1) CSB-YP761790LAAD
CSB-EP761790LAAD
CSB-BP761790LAAD
CSB-MP761790LAAD
CSB-EP761790LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Membrane protein insertase YidC 2 (yidC2), partial CSB-YP761791LAAD1
CSB-EP761791LAAD1
CSB-BP761791LAAD1
CSB-MP761791LAAD1
CSB-EP761791LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Elongation factor P (efp) CSB-YP761792LAAD
CSB-EP761792LAAD
CSB-BP761792LAAD
CSB-MP761792LAAD
CSB-EP761792LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Putative ribosome biogenesis GTPase RsgA 2 (rsgA2) CSB-YP761793LAAD
CSB-EP761793LAAD
CSB-BP761793LAAD
CSB-MP761793LAAD
CSB-EP761793LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Ribosome maturation factor RimP (rimP) CSB-YP761794LAAD
CSB-EP761794LAAD
CSB-BP761794LAAD
CSB-MP761794LAAD
CSB-EP761794LAAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria monocytogenes serotype 4b Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP761795LAAD1
CSB-EP761795LAAD1
CSB-BP761795LAAD1
CSB-MP761795LAAD1
CSB-EP761795LAAD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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