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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 50S ribosomal protein L18 (rplR) CSB-YP765882SMU
CSB-EP765882SMU
CSB-BP765882SMU
CSB-MP765882SMU
CSB-EP765882SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 50S ribosomal protein L23 (rplW) CSB-YP765883SMU
CSB-EP765883SMU
CSB-BP765883SMU
CSB-MP765883SMU
CSB-EP765883SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0391 membrane protein ytjA (ytjA), partial CSB-YP765884SXB
CSB-EP765884SXB
CSB-BP765884SXB
CSB-MP765884SXB
CSB-EP765884SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Divalent-cation tolerance protein CutA (cutA) CSB-YP765885SXB
CSB-EP765885SXB
CSB-BP765885SXB
CSB-MP765885SXB
CSB-EP765885SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium ATP synthase subunit alpha (atpA), partial CSB-YP765886SXB
CSB-EP765886SXB
CSB-BP765886SXB
CSB-MP765886SXB
CSB-EP765886SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Uncharacterized protein yicS (yicS) CSB-YP765887SXB
CSB-EP765887SXB
CSB-BP765887SXB
CSB-MP765887SXB
CSB-EP765887SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 50S ribosomal protein L18 (rplR) CSB-YP765888SXB
CSB-EP765888SXB
CSB-BP765888SXB
CSB-MP765888SXB
CSB-EP765888SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 5-keto-4-deoxy-D-glucarate aldolase (garL) CSB-YP765889SXB
CSB-EP765889SXB
CSB-BP765889SXB
CSB-MP765889SXB
CSB-EP765889SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Antitoxin VapB (vapB) CSB-YP765890SXB
CSB-EP765890SXB
CSB-BP765890SXB
CSB-MP765890SXB
CSB-EP765890SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Iron-binding protein IscA (iscA) CSB-YP765891SXB
CSB-EP765891SXB
CSB-BP765891SXB
CSB-MP765891SXB
CSB-EP765891SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP765892SXB
CSB-EP765892SXB
CSB-BP765892SXB
CSB-MP765892SXB
CSB-EP765892SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Guanine nucleotide exchange factor sopE2 (sopE2) CSB-YP765893SXB
CSB-EP765893SXB
CSB-BP765893SXB
CSB-MP765893SXB
CSB-EP765893SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Spermidine export protein MdtJ (mdtJ), partial CSB-YP765894SXB1
CSB-EP765894SXB1
CSB-BP765894SXB1
CSB-MP765894SXB1
CSB-EP765894SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Chaperone modulatory protein CbpM (cbpM) CSB-YP765895SXB
CSB-EP765895SXB
CSB-BP765895SXB
CSB-MP765895SXB
CSB-EP765895SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Isoaspartyl peptidase (iaaA) CSB-YP765896SXB
CSB-EP765896SXB
CSB-BP765896SXB
CSB-MP765896SXB
CSB-EP765896SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Putative transcriptional regulator of 2-aminoethylphosphonate degradation operons (phnR) CSB-YP765897SXB
CSB-EP765897SXB
CSB-BP765897SXB
CSB-MP765897SXB
CSB-EP765897SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Cell division protein FtsL (ftsL), partial CSB-YP765898SXB
CSB-EP765898SXB
CSB-BP765898SXB
CSB-MP765898SXB
CSB-EP765898SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens sn-glycerol-3-phosphate transport system permease protein ugpA (ugpA), partial CSB-YP765899AYS1
CSB-EP765899AYS1
CSB-BP765899AYS1
CSB-MP765899AYS1
CSB-EP765899AYS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP765900AYS
CSB-EP765900AYS
CSB-BP765900AYS
CSB-MP765900AYS
CSB-EP765900AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agrobacterium tumefaciens Putative aminoacrylate hydrolase RutD (rutD) CSB-YP765901AYS
CSB-EP765901AYS
CSB-BP765901AYS
CSB-MP765901AYS
CSB-EP765901AYS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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