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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterococcus faecalis UPF0298 protein EF_2453 (EF_2453) CSB-YP769301ELW
CSB-EP769301ELW
CSB-BP769301ELW
CSB-MP769301ELW
CSB-EP769301ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Homoserine kinase (thrB) CSB-YP769302ELW
CSB-EP769302ELW
CSB-BP769302ELW
CSB-MP769302ELW
CSB-EP769302ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 30S ribosomal protein S2 (rpsB) CSB-YP769303ELW
CSB-EP769303ELW
CSB-BP769303ELW
CSB-MP769303ELW
CSB-EP769303ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Chaperone protein ClpB (clpB), partial CSB-YP769304ELW
CSB-EP769304ELW
CSB-BP769304ELW
CSB-MP769304ELW
CSB-EP769304ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber mosaic virus RNA-directed RNA polymerase 2a (ORF2a), partial CSB-YP769305CIAQ
CSB-EP769305CIAQ
CSB-BP769305CIAQ
CSB-MP769305CIAQ
CSB-EP769305CIAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 2 (kduI2) CSB-YP769306ELW
CSB-EP769306ELW
CSB-BP769306ELW
CSB-MP769306ELW
CSB-EP769306ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP769307ELW
CSB-EP769307ELW
CSB-BP769307ELW
CSB-MP769307ELW
CSB-EP769307ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Aspartate--tRNA ligase (aspS), partial CSB-YP769308ELW
CSB-EP769308ELW
CSB-BP769308ELW
CSB-MP769308ELW
CSB-EP769308ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP769309ELW
CSB-EP769309ELW
CSB-BP769309ELW
CSB-MP769309ELW
CSB-EP769309ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Gelatinase (gelE) CSB-YP769310ELW
CSB-BP769310ELW
CSB-MP769310ELW
CSB-EP769310ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP769311ELW
CSB-EP769311ELW
CSB-BP769311ELW
CSB-MP769311ELW
CSB-EP769311ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus A Non-structural protein 1 CSB-YP769312RNY
CSB-EP769312RNY
CSB-BP769312RNY
CSB-MP769312RNY
CSB-EP769312RNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato mosaic virus Capsid protein (CP) CSB-YP769313TDAF
CSB-EP769313TDAF
CSB-BP769313TDAF
CSB-MP769313TDAF
CSB-EP769313TDAF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphate import ATP-binding protein PstB 1 (pstB1) CSB-YP769314ELW
CSB-EP769314ELW
CSB-BP769314ELW
CSB-MP769314ELW
CSB-EP769314ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Lipoprotein signal peptidase (lspA), partial CSB-YP769315ELW1
CSB-EP769315ELW1
CSB-BP769315ELW1
CSB-MP769315ELW1
CSB-EP769315ELW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Phosphate propanoyltransferase (pduL) CSB-YP769316ELW
CSB-EP769316ELW
CSB-BP769316ELW
CSB-MP769316ELW
CSB-EP769316ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Thymidylate synthase (thyA) CSB-YP769317ELW
CSB-EP769317ELW
CSB-BP769317ELW
CSB-MP769317ELW
CSB-EP769317ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis Segregation and condensation protein B (scpB) CSB-YP769318ELW
CSB-EP769318ELW
CSB-BP769318ELW
CSB-MP769318ELW
CSB-EP769318ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis V-type ATP synthase beta chain (atpB) CSB-YP769319ELW
CSB-EP769319ELW
CSB-BP769319ELW
CSB-MP769319ELW
CSB-EP769319ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterococcus faecalis UPF0173 metal-dependent hydrolase EF_1371 (EF_1371) CSB-YP769320ELW
CSB-EP769320ELW
CSB-BP769320ELW
CSB-MP769320ELW
CSB-EP769320ELW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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