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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Uncharacterized protein ygaU (ygaU) CSB-YP364963EGX
CSB-EP364963EGX
CSB-BP364963EGX
CSB-MP364963EGX
CSB-EP364963EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA-modifying protein ygfZ (ygfZ) CSB-YP364964SZB
CSB-EP364964SZB
CSB-BP364964SZB
CSB-MP364964SZB
CSB-EP364964SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative fruR/shl operon leader peptide (fruL) CSB-YP364965EGX
CSB-EP364965EGX
CSB-BP364965EGX
CSB-MP364965EGX
CSB-EP364965EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative rho operon leader peptide (rhoL) CSB-YP364966ENV
CSB-EP364966ENV
CSB-BP364966ENV
CSB-MP364966ENV
CSB-EP364966ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative rho operon leader peptide (rhoL) CSB-YP364967SZB
CSB-EP364967SZB
CSB-BP364967SZB
CSB-MP364967SZB
CSB-EP364967SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphogluconate dehydratase (edd) CSB-YP364968EOD
CSB-EP364968EOD
CSB-BP364968EOD
CSB-MP364968EOD
CSB-EP364968EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetolactate synthase isozyme 1 small subunit (ilvN) CSB-YP364969EGX
CSB-EP364969EGX
CSB-BP364969EGX
CSB-MP364969EGX
CSB-EP364969EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Acetolactate synthase isozyme 2 small subunit (ilvM) CSB-YP364970ENV
CSB-EP364970ENV
CSB-BP364970ENV
CSB-MP364970ENV
CSB-EP364970ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetolactate synthase isozyme 2 small subunit (ilvM) CSB-YP364971SZB
CSB-EP364971SZB
CSB-BP364971SZB
CSB-MP364971SZB
CSB-EP364971SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inositol-1-monophosphatase (suhB) CSB-YP364972EGX
CSB-EP364972EGX
CSB-BP364972EGX
CSB-MP364972EGX
CSB-EP364972EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inositol-1-monophosphatase (suhB) CSB-YP364973EOD
CSB-EP364973EOD
CSB-BP364973EOD
CSB-MP364973EOD
CSB-EP364973EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Insertion element IS1 protein insA (insA) CSB-YP364975SWW
CSB-EP364975SWW
CSB-BP364975SWW
CSB-MP364975SWW
CSB-EP364975SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transposase insE for insertion sequence IS3 (insE) CSB-YP364976ENZ
CSB-EP364976ENZ
CSB-BP364976ENZ
CSB-MP364976ENZ
CSB-EP364976ENZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type 1 fimbriae regulatory protein fimB (fimB) CSB-YP364977EOD
CSB-EP364977EOD
CSB-BP364977EOD
CSB-MP364977EOD
CSB-EP364977EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Type 1 fimbriae regulatory protein fimE (fimE) CSB-YP364978EGX
CSB-EP364978EGX
CSB-BP364978EGX
CSB-MP364978EGX
CSB-EP364978EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative DNA-invertase from lambdoid prophage Rac (pinR) CSB-YP364979ENV
CSB-EP364979ENV
CSB-BP364979ENV
CSB-MP364979ENV
CSB-EP364979ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposon Tn3 resolvase (tnpR) CSB-YP364980ENL
CSB-BP364980ENL
CSB-MP364980ENL
CSB-EP364980ENL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Isochorismatase (entB) CSB-YP364981ENV
CSB-EP364981ENV
CSB-BP364981ENV
CSB-MP364981ENV
CSB-EP364981ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isochorismatase (entB) CSB-YP364982SZB
CSB-EP364982SZB
CSB-BP364982SZB
CSB-MP364982SZB
CSB-EP364982SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized isochorismatase family protein yecD (yecD) CSB-YP364983EOD
CSB-EP364983EOD
CSB-BP364983EOD
CSB-MP364983EOD
CSB-EP364983EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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