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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Aeromonas hydrophila subsp. hydrophila Homoserine O-succinyltransferase (metA) CSB-YP367166AUH
CSB-EP367166AUH
CSB-BP367166AUH
CSB-MP367166AUH
CSB-EP367166AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Acetylglutamate kinase (argB) CSB-YP367167AUH
CSB-EP367167AUH
CSB-BP367167AUH
CSB-MP367167AUH
CSB-EP367167AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Arginine repressor (argR) CSB-YP367168AUH
CSB-EP367168AUH
CSB-BP367168AUH
CSB-MP367168AUH
CSB-EP367168AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Ribosome maturation factor RimM (rimM) CSB-YP367169AUH
CSB-EP367169AUH
CSB-BP367169AUH
CSB-MP367169AUH
CSB-EP367169AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila RNA pyrophosphohydrolase (rppH) CSB-YP367170AUH
CSB-EP367170AUH
CSB-BP367170AUH
CSB-MP367170AUH
CSB-EP367170AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 30S ribosomal protein S20 (rpsT) CSB-YP367171AUH
CSB-EP367171AUH
CSB-BP367171AUH
CSB-MP367171AUH
CSB-EP367171AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP367172AUH
CSB-EP367172AUH
CSB-BP367172AUH
CSB-MP367172AUH
CSB-EP367172AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila D-erythrose-4-phosphate dehydrogenase (epd) CSB-YP367173AUH
CSB-BP367173AUH
CSB-MP367173AUH
CSB-EP367173AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD CSB-YP367174AUH
CSB-EP367174AUH
CSB-BP367174AUH
CSB-MP367174AUH
CSB-EP367174AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp (gcp) CSB-YP367175AUH
CSB-EP367175AUH
CSB-BP367175AUH
CSB-MP367175AUH
CSB-EP367175AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 10 kDa chaperonin (groS) CSB-YP367176AUH
CSB-EP367176AUH
CSB-BP367176AUH
CSB-MP367176AUH
CSB-EP367176AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP367177AUH
CSB-EP367177AUH
CSB-BP367177AUH
CSB-MP367177AUH
CSB-EP367177AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Protein hfq (hfq) CSB-YP367178AUH
CSB-EP367178AUH
CSB-BP367178AUH
CSB-MP367178AUH
CSB-EP367178AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Bis (5'-nucleosyl)-tetraphosphatase, symmetrical CSB-YP367179AUH
CSB-EP367179AUH
CSB-BP367179AUH
CSB-MP367179AUH
CSB-EP367179AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE (arnE), partial CSB-YP367180AUH1
CSB-EP367180AUH1
CSB-BP367180AUH1
CSB-MP367180AUH1
CSB-EP367180AUH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0270 protein AHA_0994 (AHA_0994) CSB-YP367181AUH
CSB-EP367181AUH
CSB-BP367181AUH
CSB-MP367181AUH
CSB-EP367181AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Na (+)-translocating NADH-quinone reductase subunit E, partial CSB-YP367182AUH1
CSB-EP367182AUH1
CSB-BP367182AUH1
CSB-MP367182AUH1
CSB-EP367182AUH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Maltoporin (lamB) CSB-YP367183AUH
CSB-EP367183AUH
CSB-BP367183AUH
CSB-MP367183AUH
CSB-EP367183AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 30S ribosomal protein S2 (rpsB) CSB-YP367184AUH
CSB-EP367184AUH
CSB-BP367184AUH
CSB-MP367184AUH
CSB-EP367184AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr) CSB-YP367185AUH
CSB-EP367185AUH
CSB-BP367185AUH
CSB-MP367185AUH
CSB-EP367185AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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