Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium ulcerans Sec-independent protein translocase protein TatA (tatA), partial CSB-YP367626MOP
CSB-EP367626MOP
CSB-BP367626MOP
CSB-MP367626MOP
CSB-EP367626MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Proteasome subunit beta (prcB) CSB-YP367627MOP
CSB-EP367627MOP
CSB-BP367627MOP
CSB-MP367627MOP
CSB-EP367627MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Phosphoribosyl-ATP pyrophosphatase (hisE) CSB-YP367628MOP
CSB-EP367628MOP
CSB-BP367628MOP
CSB-MP367628MOP
CSB-EP367628MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (mshC) CSB-YP367629MOP
CSB-EP367629MOP
CSB-BP367629MOP
CSB-MP367629MOP
CSB-EP367629MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Phthiotriol/phenolphthiotriol dimycocerosates methyltransferase 1 (MUL_2377) CSB-YP367630MOP
CSB-EP367630MOP
CSB-BP367630MOP
CSB-MP367630MOP
CSB-EP367630MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Acetaldehyde dehydrogenase 1 (mhpF) CSB-YP367631MOP
CSB-EP367631MOP
CSB-BP367631MOP
CSB-MP367631MOP
CSB-EP367631MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP367632MOP
CSB-EP367632MOP
CSB-BP367632MOP
CSB-MP367632MOP
CSB-EP367632MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP367633MOP
CSB-EP367633MOP
CSB-BP367633MOP
CSB-MP367633MOP
CSB-EP367633MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans UPF0182 protein MUL_2505 (MUL_2505), partial CSB-YP367634MOP
CSB-EP367634MOP
CSB-BP367634MOP
CSB-MP367634MOP
CSB-EP367634MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Protein translocase subunit SecA 1 (secA1), partial CSB-YP367635MOP
CSB-EP367635MOP
CSB-BP367635MOP
CSB-MP367635MOP
CSB-EP367635MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 2-phospho-L-lactate transferase (cofD) CSB-YP367636MOP
CSB-EP367636MOP
CSB-BP367636MOP
CSB-MP367636MOP
CSB-EP367636MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative S-adenosyl-L-methionine-dependent methyltransferase MUL_2766 (MUL_2766) CSB-YP367637MOP
CSB-EP367637MOP
CSB-BP367637MOP
CSB-MP367637MOP
CSB-EP367637MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Adenylosuccinate synthetase (purA) CSB-YP367638MOP
CSB-EP367638MOP
CSB-BP367638MOP
CSB-MP367638MOP
CSB-EP367638MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Alkyl hydroperoxide reductase AhpD (ahpD) CSB-YP367639MOP
CSB-EP367639MOP
CSB-BP367639MOP
CSB-MP367639MOP
CSB-EP367639MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Urease subunit beta (ureB) CSB-YP367640MOP
CSB-EP367640MOP
CSB-BP367640MOP
CSB-MP367640MOP
CSB-EP367640MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Malate synthase G (glcB), partial CSB-YP367641MOP
CSB-EP367641MOP
CSB-BP367641MOP
CSB-MP367641MOP
CSB-EP367641MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Probable transcriptional regulatory protein MUL_3246 (MUL_3246) CSB-YP367642MOP
CSB-EP367642MOP
CSB-BP367642MOP
CSB-MP367642MOP
CSB-EP367642MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP367643MOP
CSB-EP367643MOP
CSB-BP367643MOP
CSB-MP367643MOP
CSB-EP367643MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Transcriptional repressor NrdR (nrdR) CSB-YP367644MOP
CSB-EP367644MOP
CSB-BP367644MOP
CSB-MP367644MOP
CSB-EP367644MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans tRNA dimethylallyltransferase 1 (miaA1) CSB-YP367645MOP
CSB-EP367645MOP
CSB-BP367645MOP
CSB-MP367645MOP
CSB-EP367645MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>