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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Aeromonas hydrophila subsp. hydrophila Protein TolB (tolB) CSB-YP368506AUH
CSB-EP368506AUH
CSB-BP368506AUH
CSB-MP368506AUH
CSB-EP368506AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP368507AUH
CSB-EP368507AUH
CSB-BP368507AUH
CSB-MP368507AUH
CSB-EP368507AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Protein RecA (recA) CSB-YP368508AUH
CSB-EP368508AUH
CSB-BP368508AUH
CSB-MP368508AUH
CSB-EP368508AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Zinc import ATP-binding protein ZnuC (znuC) CSB-YP368509AUH
CSB-EP368509AUH
CSB-BP368509AUH
CSB-MP368509AUH
CSB-EP368509AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila DNA gyrase inhibitor YacG (yacG) CSB-YP368510AUH
CSB-EP368510AUH
CSB-BP368510AUH
CSB-MP368510AUH
CSB-EP368510AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP368511AUH
CSB-EP368511AUH
CSB-BP368511AUH
CSB-MP368511AUH
CSB-EP368511AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0042 nucleotide-binding protein AHA_3920 (AHA_3920) CSB-YP368512AUH
CSB-EP368512AUH
CSB-BP368512AUH
CSB-MP368512AUH
CSB-EP368512AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 30S ribosomal protein S7 (rpsG) CSB-YP368513AUH
CSB-EP368513AUH
CSB-BP368513AUH
CSB-MP368513AUH
CSB-EP368513AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila 50S ribosomal protein L7/L12 (rplL) CSB-YP368514AUH
CSB-EP368514AUH
CSB-BP368514AUH
CSB-MP368514AUH
CSB-EP368514AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0597 protein AHA_4077 (AHA_4077) CSB-YP368515AUH
CSB-EP368515AUH
CSB-BP368515AUH
CSB-MP368515AUH
CSB-EP368515AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila ATP-dependent protease subunit HslV (hslV) CSB-YP368516AUH
CSB-EP368516AUH
CSB-BP368516AUH
CSB-MP368516AUH
CSB-EP368516AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila UPF0061 protein AHA_4127 (AHA_4127) CSB-YP368517AUH
CSB-EP368517AUH
CSB-BP368517AUH
CSB-MP368517AUH
CSB-EP368517AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP368518AUH
CSB-EP368518AUH
CSB-BP368518AUH
CSB-MP368518AUH
CSB-EP368518AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aeromonas hydrophila subsp. hydrophila Ribonuclease P protein component (rnpA) CSB-YP368519AUH
CSB-EP368519AUH
CSB-BP368519AUH
CSB-MP368519AUH
CSB-EP368519AUH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP368520STS
CSB-EP368520STS
CSB-BP368520STS
CSB-MP368520STS
CSB-EP368520STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Membrane protein insertase YidC (yidC), partial CSB-YP368521STS1
CSB-EP368521STS1
CSB-BP368521STS1
CSB-MP368521STS1
CSB-EP368521STS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Ribonuclease P protein component (rnpA) CSB-YP368522STS
CSB-EP368522STS
CSB-BP368522STS
CSB-MP368522STS
CSB-EP368522STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP368523STS
CSB-EP368523STS
CSB-BP368523STS
CSB-MP368523STS
CSB-EP368523STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Protein smg homolog (smg) CSB-YP368524STS
CSB-EP368524STS
CSB-BP368524STS
CSB-MP368524STS
CSB-EP368524STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP368525STS
CSB-EP368525STS
CSB-BP368525STS
CSB-MP368525STS
CSB-EP368525STS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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