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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidothermus cellulolyticus 50S ribosomal protein L28 (rpmB) CSB-YP369346AUA
CSB-EP369346AUA
CSB-BP369346AUA
CSB-MP369346AUA
CSB-EP369346AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP369347AUA
CSB-EP369347AUA
CSB-BP369347AUA
CSB-MP369347AUA
CSB-EP369347AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Peptide chain release factor 2 (prfB) CSB-YP369348AUA
CSB-EP369348AUA
CSB-BP369348AUA
CSB-MP369348AUA
CSB-EP369348AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Glucose-1-phosphate adenylyltransferase (glgC) CSB-YP369349AUA
CSB-EP369349AUA
CSB-BP369349AUA
CSB-MP369349AUA
CSB-EP369349AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Serine--tRNA ligase (serS) CSB-YP369350AUA
CSB-EP369350AUA
CSB-BP369350AUA
CSB-MP369350AUA
CSB-EP369350AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus Deoxycytidine triphosphate deaminase (dcd) CSB-YP369351AUA
CSB-EP369351AUA
CSB-BP369351AUA
CSB-MP369351AUA
CSB-EP369351AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 30S ribosomal protein S6 (rpsF) CSB-YP369352AUA
CSB-EP369352AUA
CSB-BP369352AUA
CSB-MP369352AUA
CSB-EP369352AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidothermus cellulolyticus 50S ribosomal protein L34 (rpmH) CSB-YP369353AUA
CSB-EP369353AUA
CSB-BP369353AUA
CSB-MP369353AUA
CSB-EP369353AUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Uridylate kinase (pyrH) CSB-YP369354GEQ
CSB-EP369354GEQ
CSB-BP369354GEQ
CSB-MP369354GEQ
CSB-EP369354GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Ribosome maturation factor RimP (rimP) CSB-YP369355GEQ
CSB-EP369355GEQ
CSB-BP369355GEQ
CSB-MP369355GEQ
CSB-EP369355GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP369356GEQ
CSB-EP369356GEQ
CSB-BP369356GEQ
CSB-MP369356GEQ
CSB-EP369356GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Probable inorganic polyphosphate/ATP-NAD kinase (ppnK) CSB-YP369357GEQ
CSB-EP369357GEQ
CSB-BP369357GEQ
CSB-MP369357GEQ
CSB-EP369357GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii S-adenosylmethionine synthase (metK) CSB-YP369358GEQ
CSB-EP369358GEQ
CSB-BP369358GEQ
CSB-MP369358GEQ
CSB-EP369358GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii D-tyrosyl-tRNA (Tyr) deacylase CSB-YP369359GEQ
CSB-EP369359GEQ
CSB-BP369359GEQ
CSB-MP369359GEQ
CSB-EP369359GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii 30S ribosomal protein S15 (rpsO) CSB-YP369360GEQ
CSB-EP369360GEQ
CSB-BP369360GEQ
CSB-MP369360GEQ
CSB-EP369360GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP369361GEQ
CSB-EP369361GEQ
CSB-BP369361GEQ
CSB-MP369361GEQ
CSB-EP369361GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii tRNA (guanine-N (1)-)-methyltransferase CSB-YP369362GEQ
CSB-EP369362GEQ
CSB-BP369362GEQ
CSB-MP369362GEQ
CSB-EP369362GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Imidazolonepropionase (hutI) CSB-YP369363GEQ
CSB-EP369363GEQ
CSB-BP369363GEQ
CSB-MP369363GEQ
CSB-EP369363GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii 50S ribosomal protein L34 (rpmH) CSB-YP369364GEQ
CSB-EP369364GEQ
CSB-BP369364GEQ
CSB-MP369364GEQ
CSB-EP369364GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gramella forsetii Non-canonical purine NTP pyrophosphatase (GFO_1100) CSB-YP369365GEQ
CSB-EP369365GEQ
CSB-BP369365GEQ
CSB-MP369365GEQ
CSB-EP369365GEQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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