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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium ulcerans Crossover junction endodeoxyribonuclease RuvC (ruvC) CSB-YP369446MOP
CSB-EP369446MOP
CSB-BP369446MOP
CSB-MP369446MOP
CSB-EP369446MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP369447MOP
CSB-EP369447MOP
CSB-BP369447MOP
CSB-MP369447MOP
CSB-EP369447MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Putative Holliday junction resolvase (MUL_1755) CSB-YP369448MOP
CSB-EP369448MOP
CSB-BP369448MOP
CSB-MP369448MOP
CSB-EP369448MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Chorismate synthase (aroC) CSB-YP369449MOP
CSB-EP369449MOP
CSB-BP369449MOP
CSB-MP369449MOP
CSB-EP369449MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Bifunctional protein pyrR (pyrR) CSB-YP369450MOP
CSB-EP369450MOP
CSB-BP369450MOP
CSB-MP369450MOP
CSB-EP369450MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Orotidine 5'-phosphate decarboxylase (pyrF) CSB-YP369451MOP
CSB-EP369451MOP
CSB-BP369451MOP
CSB-MP369451MOP
CSB-EP369451MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Riboflavin biosynthesis protein ribBA (ribBA) CSB-YP369452MOP
CSB-EP369452MOP
CSB-BP369452MOP
CSB-MP369452MOP
CSB-EP369452MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans UPF0042 nucleotide-binding protein MUL_1815 (MUL_1815) CSB-YP369453MOP
CSB-EP369453MOP
CSB-BP369453MOP
CSB-MP369453MOP
CSB-EP369453MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Phosphoglycerate kinase (pgk) CSB-YP369454MOP
CSB-EP369454MOP
CSB-BP369454MOP
CSB-MP369454MOP
CSB-EP369454MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans DNA ligase (ligA), partial CSB-YP369455MOP
CSB-EP369455MOP
CSB-BP369455MOP
CSB-MP369455MOP
CSB-EP369455MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Glutamate--tRNA ligase (gltX) CSB-YP369456MOP
CSB-EP369456MOP
CSB-BP369456MOP
CSB-MP369456MOP
CSB-EP369456MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Polyphosphate kinase (ppk), partial CSB-YP369457MOP
CSB-EP369457MOP
CSB-BP369457MOP
CSB-MP369457MOP
CSB-EP369457MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Uncharacterized oxidoreductase MUL_1987 (MUL_1987) CSB-YP369458MOP
CSB-EP369458MOP
CSB-BP369458MOP
CSB-MP369458MOP
CSB-EP369458MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Trans-acting enoyl reductase (MUL_2000) CSB-YP369459MOP
CSB-EP369459MOP
CSB-BP369459MOP
CSB-MP369459MOP
CSB-EP369459MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Ribosome maturation factor RimM (rimM) CSB-YP369460MOP
CSB-EP369460MOP
CSB-BP369460MOP
CSB-MP369460MOP
CSB-EP369460MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans 30S ribosomal protein S2 (rpsB) CSB-YP369461MOP
CSB-EP369461MOP
CSB-BP369461MOP
CSB-MP369461MOP
CSB-EP369461MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Ribosome maturation factor RimP (rimP) CSB-YP369462MOP
CSB-EP369462MOP
CSB-BP369462MOP
CSB-MP369462MOP
CSB-EP369462MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Ribosome-binding factor A (rbfA) CSB-YP369463MOP
CSB-EP369463MOP
CSB-BP369463MOP
CSB-MP369463MOP
CSB-EP369463MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Dihydrodipicolinate reductase (dapB) CSB-YP369464MOP
CSB-EP369464MOP
CSB-BP369464MOP
CSB-MP369464MOP
CSB-EP369464MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium ulcerans Proteasome subunit alpha (prcA) CSB-YP369465MOP
CSB-EP369465MOP
CSB-BP369465MOP
CSB-MP369465MOP
CSB-EP369465MOP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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