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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Marinobacter aquaeolei UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP378890MNI
CSB-EP378890MNI
CSB-BP378890MNI
CSB-MP378890MNI
CSB-EP378890MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP378891MNI
CSB-EP378891MNI
CSB-BP378891MNI
CSB-MP378891MNI
CSB-EP378891MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Protein MraZ (mraZ) CSB-YP378892MNI
CSB-EP378892MNI
CSB-BP378892MNI
CSB-MP378892MNI
CSB-EP378892MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 30S ribosomal protein S9 (rpsI) CSB-YP378893MNI
CSB-EP378893MNI
CSB-BP378893MNI
CSB-MP378893MNI
CSB-EP378893MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 3-demethylubiquinone-9 3-methyltransferase (ubiG) CSB-YP378894MNI
CSB-EP378894MNI
CSB-BP378894MNI
CSB-MP378894MNI
CSB-EP378894MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Dihydroorotase (pyrC) CSB-YP378895MNI
CSB-EP378895MNI
CSB-BP378895MNI
CSB-MP378895MNI
CSB-EP378895MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ribonuclease HII (rnhB) CSB-YP378896MNI
CSB-EP378896MNI
CSB-BP378896MNI
CSB-MP378896MNI
CSB-EP378896MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Sulfate adenylyltransferase subunit 2 1 (cysD1) CSB-YP378897MNI
CSB-EP378897MNI
CSB-BP378897MNI
CSB-MP378897MNI
CSB-EP378897MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UPF0042 nucleotide-binding protein Maqu_2718 (Maqu_2718) CSB-YP378898MNI
CSB-EP378898MNI
CSB-BP378898MNI
CSB-MP378898MNI
CSB-EP378898MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Glutamyl-tRNA (Gln) amidotransferase subunit A CSB-YP378899MNI
CSB-EP378899MNI
CSB-BP378899MNI
CSB-MP378899MNI
CSB-EP378899MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP378900MNI
CSB-EP378900MNI
CSB-BP378900MNI
CSB-MP378900MNI
CSB-EP378900MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB CSB-YP378901MNI
CSB-EP378901MNI
CSB-BP378901MNI
CSB-MP378901MNI
CSB-EP378901MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Leucine--tRNA ligase (leuS), partial CSB-YP378902MNI
CSB-EP378902MNI
CSB-BP378902MNI
CSB-MP378902MNI
CSB-EP378902MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei 8-amino-7-oxononanoate synthase (bioF) CSB-YP378903MNI
CSB-EP378903MNI
CSB-BP378903MNI
CSB-MP378903MNI
CSB-EP378903MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei tRNA (cmo5U34)-methyltransferase CSB-YP378904MNI
CSB-EP378904MNI
CSB-BP378904MNI
CSB-MP378904MNI
CSB-EP378904MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei tRNA (mo5U34)-methyltransferase CSB-YP378905MNI
CSB-EP378905MNI
CSB-BP378905MNI
CSB-MP378905MNI
CSB-EP378905MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP378906MNI
CSB-EP378906MNI
CSB-BP378906MNI
CSB-MP378906MNI
CSB-EP378906MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UPF0345 protein Maqu_2862 (Maqu_2862) CSB-YP378907MNI
CSB-EP378907MNI
CSB-BP378907MNI
CSB-MP378907MNI
CSB-EP378907MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei UPF0391 membrane protein Maqu_2901 (Maqu_2901), partial CSB-YP378908MNI
CSB-EP378908MNI
CSB-BP378908MNI
CSB-MP378908MNI
CSB-EP378908MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinobacter aquaeolei Urease accessory protein UreF (ureF) CSB-YP378909MNI
CSB-EP378909MNI
CSB-BP378909MNI
CSB-MP378909MNI
CSB-EP378909MNI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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