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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Hyperthermus butylicus Translation initiation factor 5A (eIF5A) CSB-YP381230HSR
CSB-EP381230HSR
CSB-BP381230HSR
CSB-MP381230HSR
CSB-EP381230HSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein grpE (grpE) CSB-YP381231PZB
CSB-EP381231PZB
CSB-BP381231PZB
CSB-MP381231PZB
CSB-EP381231PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Elongation factor P (efp) CSB-YP381232PZB
CSB-EP381232PZB
CSB-BP381232PZB
CSB-MP381232PZB
CSB-EP381232PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Biosynthetic arginine decarboxylase (speA), partial CSB-YP381233PZB
CSB-EP381233PZB
CSB-BP381233PZB
CSB-MP381233PZB
CSB-EP381233PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase chain 4, partial CSB-YP381234PZB
CSB-EP381234PZB
CSB-BP381234PZB
CSB-MP381234PZB
CSB-EP381234PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit 1, partial CSB-YP381235PZB
CSB-EP381235PZB
CSB-BP381235PZB
CSB-MP381235PZB
CSB-EP381235PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP381236PZB
CSB-EP381236PZB
CSB-BP381236PZB
CSB-MP381236PZB
CSB-EP381236PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Probable rRNA maturation factor (A9601_02001) CSB-YP381237PZB
CSB-EP381237PZB
CSB-BP381237PZB
CSB-MP381237PZB
CSB-EP381237PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP381238PZB
CSB-EP381238PZB
CSB-BP381238PZB
CSB-MP381238PZB
CSB-EP381238PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus 50S ribosomal protein L1 (rplA) CSB-YP381239PZB
CSB-EP381239PZB
CSB-BP381239PZB
CSB-MP381239PZB
CSB-EP381239PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Chorismate synthase (aroC) CSB-YP381240PZB
CSB-EP381240PZB
CSB-BP381240PZB
CSB-MP381240PZB
CSB-EP381240PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus tRNA (guanine-N (7)-)-methyltransferase CSB-YP381241PZB
CSB-EP381241PZB
CSB-BP381241PZB
CSB-MP381241PZB
CSB-EP381241PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein H (psbH), partial CSB-YP381242PZB1
CSB-EP381242PZB1
CSB-BP381242PZB1
CSB-MP381242PZB1
CSB-EP381242PZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Protein psbN (psbN), partial CSB-YP381243PZB
CSB-EP381243PZB
CSB-BP381243PZB
CSB-MP381243PZB
CSB-EP381243PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Sugar fermentation stimulation protein homolog (sfsA) CSB-YP381244PZB
CSB-EP381244PZB
CSB-BP381244PZB
CSB-MP381244PZB
CSB-EP381244PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein K (psbK) CSB-YP381245PZB
CSB-EP381245PZB
CSB-BP381245PZB
CSB-MP381245PZB
CSB-EP381245PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus NAD (P)H-quinone oxidoreductase subunit K CSB-YP381246PZB
CSB-EP381246PZB
CSB-BP381246PZB
CSB-MP381246PZB
CSB-EP381246PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cytochrome b559 subunit alpha (psbE), partial CSB-YP381247PZB1
CSB-EP381247PZB1
CSB-BP381247PZB1
CSB-MP381247PZB1
CSB-EP381247PZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Photosystem II reaction center protein T (psbT), partial CSB-YP381248PZB
CSB-EP381248PZB
CSB-BP381248PZB
CSB-MP381248PZB
CSB-EP381248PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prochlorococcus marinus Cell division topological specificity factor (minE) CSB-YP381249PZB
CSB-EP381249PZB
CSB-BP381249PZB
CSB-MP381249PZB
CSB-EP381249PZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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