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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lactobacillus reuteri Phosphate propanoyltransferase (pduL) CSB-YP404750LLF
CSB-EP404750LLF
CSB-BP404750LLF
CSB-MP404750LLF
CSB-EP404750LLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Lipoprotein signal peptidase (lspA), partial CSB-YP404751BPI1
CSB-EP404751BPI1
CSB-BP404751BPI1
CSB-MP404751BPI1
CSB-EP404751BPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Sulfate adenylyltransferase subunit 2 (cysD) CSB-YP404752BPI
CSB-EP404752BPI
CSB-BP404752BPI
CSB-MP404752BPI
CSB-EP404752BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Urease accessory protein UreG 1 (ureG1) CSB-YP404753BPI
CSB-EP404753BPI
CSB-BP404753BPI
CSB-MP404753BPI
CSB-EP404753BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Uncharacterized protein BOV_0353 (BOV_0353) CSB-YP404754BPI
CSB-EP404754BPI
CSB-BP404754BPI
CSB-MP404754BPI
CSB-EP404754BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP synthase subunit b 1 (atpF1), partial CSB-YP404755BPI1
CSB-EP404755BPI1
CSB-BP404755BPI1
CSB-MP404755BPI1
CSB-EP404755BPI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S18 (rpsR) CSB-YP404756BPI
CSB-EP404756BPI
CSB-BP404756BPI
CSB-MP404756BPI
CSB-EP404756BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Ureidoglycolate hydrolase (allA) CSB-YP404757BPI
CSB-EP404757BPI
CSB-BP404757BPI
CSB-MP404757BPI
CSB-EP404757BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP404758BPI
CSB-EP404758BPI
CSB-BP404758BPI
CSB-MP404758BPI
CSB-EP404758BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP404759BPI
CSB-EP404759BPI
CSB-BP404759BPI
CSB-MP404759BPI
CSB-EP404759BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Probable cytosol aminopeptidase (pepA) CSB-YP404760BPI
CSB-EP404760BPI
CSB-BP404760BPI
CSB-MP404760BPI
CSB-EP404760BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Aspartate--tRNA ligase (aspS), partial CSB-YP404761BPI
CSB-EP404761BPI
CSB-BP404761BPI
CSB-MP404761BPI
CSB-EP404761BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S9 (rpsI) CSB-YP404762BPI
CSB-EP404762BPI
CSB-BP404762BPI
CSB-MP404762BPI
CSB-EP404762BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Deoxyguanosinetriphosphate triphosphohydrolase-like protein (BOV_0867) CSB-YP404763BPI
CSB-EP404763BPI
CSB-BP404763BPI
CSB-MP404763BPI
CSB-EP404763BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Molybdenum cofactor biosynthesis protein A (moaA) CSB-YP404764BPI
CSB-EP404764BPI
CSB-BP404764BPI
CSB-MP404764BPI
CSB-EP404764BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP404765BPI
CSB-EP404765BPI
CSB-BP404765BPI
CSB-MP404765BPI
CSB-EP404765BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 2-dehydro-3-deoxyphosphooctonate aldolase (kdsA) CSB-YP404766BPI
CSB-EP404766BPI
CSB-BP404766BPI
CSB-MP404766BPI
CSB-EP404766BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S13 (rpsM) CSB-YP404767BPI
CSB-EP404767BPI
CSB-BP404767BPI
CSB-MP404767BPI
CSB-EP404767BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L14 (rplN) CSB-YP404768BPI
CSB-EP404768BPI
CSB-BP404768BPI
CSB-MP404768BPI
CSB-EP404768BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L1 (rplA) CSB-YP404769BPI
CSB-EP404769BPI
CSB-BP404769BPI
CSB-MP404769BPI
CSB-EP404769BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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