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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ochrobactrum anthropi Cobalamin synthase (cobS), partial CSB-YP408092ODH1
CSB-EP408092ODH1
CSB-BP408092ODH1
CSB-MP408092ODH1
CSB-EP408092ODH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Na (+)/H (+) antiporter nhaA 2, partial CSB-YP408093ODH
CSB-EP408093ODH
CSB-BP408093ODH
CSB-MP408093ODH
CSB-EP408093ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Probable cytosol aminopeptidase (pepA) CSB-YP408094ODH
CSB-EP408094ODH
CSB-BP408094ODH
CSB-MP408094ODH
CSB-EP408094ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Biotin synthase (bioB) CSB-YP408095ODH
CSB-EP408095ODH
CSB-BP408095ODH
CSB-MP408095ODH
CSB-EP408095ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP408096ODH
CSB-EP408096ODH
CSB-BP408096ODH
CSB-MP408096ODH
CSB-EP408096ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Xylose isomerase (xylA) CSB-YP408097ODH
CSB-EP408097ODH
CSB-BP408097ODH
CSB-MP408097ODH
CSB-EP408097ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Ribosomal protein S12 methylthiotransferase RimO (rimO) CSB-YP408098ODH
CSB-EP408098ODH
CSB-BP408098ODH
CSB-MP408098ODH
CSB-EP408098ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ochrobactrum anthropi Putative Holliday junction resolvase (Oant_3664) CSB-YP408099ODH
CSB-EP408099ODH
CSB-BP408099ODH
CSB-MP408099ODH
CSB-EP408099ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Rho GTPase-activating protein 31 (Arhgap31), partial CSB-YP408100MO
CSB-EP408100MO
CSB-BP408100MO
CSB-MP408100MO
CSB-EP408100MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative uncharacterized protein C27E2.13 (SPAC27E2.13) CSB-YP408101SXV
CSB-EP408101SXV
CSB-BP408101SXV
CSB-MP408101SXV
CSB-EP408101SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative uncharacterized protein C188.05 (SPCC188.05) CSB-YP408102SXV
CSB-EP408102SXV
CSB-BP408102SXV
CSB-MP408102SXV
CSB-EP408102SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP408103LLQ
CSB-EP408103LLQ
CSB-BP408103LLQ
CSB-MP408103LLQ
CSB-EP408103LLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP408104LLQ1
CSB-EP408104LLQ1
CSB-BP408104LLQ1
CSB-MP408104LLQ1
CSB-EP408104LLQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP408105LLQ
CSB-EP408105LLQ
CSB-BP408105LLQ
CSB-MP408105LLQ
CSB-EP408105LLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris 30S ribosomal protein S7, chloroplastic (rps7) CSB-YP408106LLQ
CSB-EP408106LLQ
CSB-BP408106LLQ
CSB-MP408106LLQ
CSB-EP408106LLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris 50S ribosomal protein L16, chloroplastic (rpl16) CSB-YP408107LLQ
CSB-EP408107LLQ
CSB-BP408107LLQ
CSB-MP408107LLQ
CSB-EP408107LLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leptosira terrestris 30S ribosomal protein S11, chloroplastic (rps11) CSB-YP408108LLQ
CSB-EP408108LLQ
CSB-BP408108LLQ
CSB-MP408108LLQ
CSB-EP408108LLQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus californicus Mu-conotoxin-like Cal 12.1.2a CSB-YP408109DVU
CSB-EP408109DVU
CSB-BP408109DVU
CSB-MP408109DVU
CSB-EP408109DVU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pleurotus pulmonarius Trehalose phosphorylase (TP), partial CSB-YP408110EXC
CSB-EP408110EXC
CSB-BP408110EXC
CSB-MP408110EXC
CSB-EP408110EXC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitotic spindle-associated protein SHE1 (SHE1) CSB-YP408111STA
CSB-EP408111STA
CSB-BP408111STA
CSB-MP408111STA
CSB-EP408111STA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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