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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kineococcus radiotolerans Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP411213KAW
CSB-EP411213KAW
CSB-BP411213KAW
CSB-MP411213KAW
CSB-EP411213KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP411214KAW
CSB-EP411214KAW
CSB-BP411214KAW
CSB-MP411214KAW
CSB-EP411214KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Ribonuclease Z (rnz) CSB-YP411215KAW
CSB-EP411215KAW
CSB-BP411215KAW
CSB-MP411215KAW
CSB-EP411215KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans 50S ribosomal protein L34 (rpmH) CSB-YP411216KAW
CSB-EP411216KAW
CSB-BP411216KAW
CSB-MP411216KAW
CSB-EP411216KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Glycerol-3-phosphate dehydrogenase [NAD (P)+] CSB-YP411217STM
CSB-EP411217STM
CSB-BP411217STM
CSB-MP411217STM
CSB-EP411217STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 33 kDa chaperonin (hslO) CSB-YP411218STM
CSB-EP411218STM
CSB-BP411218STM
CSB-MP411218STM
CSB-EP411218STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L1 (rplA) CSB-YP411219STM
CSB-EP411219STM
CSB-BP411219STM
CSB-MP411219STM
CSB-EP411219STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S7 (rpsG) CSB-YP411220STM
CSB-EP411220STM
CSB-BP411220STM
CSB-MP411220STM
CSB-EP411220STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S3 (rpsC) CSB-YP411221STM
CSB-EP411221STM
CSB-BP411221STM
CSB-MP411221STM
CSB-EP411221STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L18 (rplR) CSB-YP411222STM
CSB-EP411222STM
CSB-BP411222STM
CSB-MP411222STM
CSB-EP411222STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S4 (rpsD) CSB-YP411223STM
CSB-EP411223STM
CSB-BP411223STM
CSB-MP411223STM
CSB-EP411223STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica FMN-dependent NADH-azoreductase (azoR) CSB-YP411224STM
CSB-EP411224STM
CSB-BP411224STM
CSB-MP411224STM
CSB-EP411224STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 50S ribosomal protein L33 (rpmG) CSB-YP411225STM
CSB-EP411225STM
CSB-BP411225STM
CSB-MP411225STM
CSB-EP411225STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP411226STM
CSB-EP411226STM
CSB-BP411226STM
CSB-MP411226STM
CSB-EP411226STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Ubiquinone/menaquinone biosynthesis methyltransferase ubiE (ubiE) CSB-YP411227STM
CSB-EP411227STM
CSB-BP411227STM
CSB-MP411227STM
CSB-EP411227STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica UPF0042 nucleotide-binding protein Shew185_0683 (Shew185_0683) CSB-YP411228STM
CSB-EP411228STM
CSB-BP411228STM
CSB-MP411228STM
CSB-EP411228STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica PKHD-type hydroxylase Shew185_0721 (Shew185_0721) CSB-YP411229STM
CSB-EP411229STM
CSB-BP411229STM
CSB-MP411229STM
CSB-EP411229STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Adenylyl-sulfate kinase (cysC) CSB-YP411230STM
CSB-EP411230STM
CSB-BP411230STM
CSB-MP411230STM
CSB-EP411230STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica GTPase obg (obg) CSB-YP411231STM
CSB-EP411231STM
CSB-BP411231STM
CSB-MP411231STM
CSB-EP411231STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica 30S ribosomal protein S20 (rpsT) CSB-YP411232STM
CSB-EP411232STM
CSB-BP411232STM
CSB-MP411232STM
CSB-EP411232STM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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