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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Nitratiruptor sp. Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP412354NFA
CSB-EP412354NFA
CSB-BP412354NFA
CSB-MP412354NFA
CSB-EP412354NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Dihydroxy-acid dehydratase (ilvD), partial CSB-YP412355NFA
CSB-EP412355NFA
CSB-BP412355NFA
CSB-MP412355NFA
CSB-EP412355NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 30S ribosomal protein S10 (rpsJ) CSB-YP412356NFA
CSB-EP412356NFA
CSB-BP412356NFA
CSB-MP412356NFA
CSB-EP412356NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 30S ribosomal protein S17 (rpsQ) CSB-YP412357NFA
CSB-EP412357NFA
CSB-BP412357NFA
CSB-MP412357NFA
CSB-EP412357NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Translation initiation factor IF-1 (infA) CSB-YP412358NFA
CSB-EP412358NFA
CSB-BP412358NFA
CSB-MP412358NFA
CSB-EP412358NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Probable endonuclease 4 (nfo) CSB-YP412359NFA
CSB-EP412359NFA
CSB-BP412359NFA
CSB-MP412359NFA
CSB-EP412359NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 50S ribosomal protein L28 (rpmB) CSB-YP412360NFA
CSB-EP412360NFA
CSB-BP412360NFA
CSB-MP412360NFA
CSB-EP412360NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP412361NFA
CSB-EP412361NFA
CSB-BP412361NFA
CSB-MP412361NFA
CSB-EP412361NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 50S ribosomal protein L32 (rpmF) CSB-YP412362NFA
CSB-EP412362NFA
CSB-BP412362NFA
CSB-MP412362NFA
CSB-EP412362NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP412363NFA
CSB-EP412363NFA
CSB-BP412363NFA
CSB-MP412363NFA
CSB-EP412363NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Bifunctional enzyme IspD/IspF (ispDF) CSB-YP412364NFA
CSB-EP412364NFA
CSB-BP412364NFA
CSB-MP412364NFA
CSB-EP412364NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. tRNA (uracil (54)-C (5))-methyltransferase CSB-YP412365NFA
CSB-EP412365NFA
CSB-BP412365NFA
CSB-MP412365NFA
CSB-EP412365NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. 7-cyano-7-deazaguanine synthase (queC) CSB-YP412366NFA
CSB-EP412366NFA
CSB-BP412366NFA
CSB-MP412366NFA
CSB-EP412366NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP412367NFA
CSB-EP412367NFA
CSB-BP412367NFA
CSB-MP412367NFA
CSB-EP412367NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Valine--tRNA ligase (valS), partial CSB-YP412368NFA
CSB-EP412368NFA
CSB-BP412368NFA
CSB-MP412368NFA
CSB-EP412368NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Ribonuclease Y (rny), partial CSB-YP412369NFA
CSB-EP412369NFA
CSB-BP412369NFA
CSB-MP412369NFA
CSB-EP412369NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Chaperone protein DnaK (dnaK), partial CSB-YP412370NFA
CSB-EP412370NFA
CSB-BP412370NFA
CSB-MP412370NFA
CSB-EP412370NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) CSB-YP412371NFA
CSB-EP412371NFA
CSB-BP412371NFA
CSB-MP412371NFA
CSB-EP412371NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Ribosomal RNA large subunit methyltransferase H (rlmH) CSB-YP412372NFA
CSB-EP412372NFA
CSB-BP412372NFA
CSB-MP412372NFA
CSB-EP412372NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. GTPase obg (obg) CSB-YP412373NFA
CSB-EP412373NFA
CSB-BP412373NFA
CSB-MP412373NFA
CSB-EP412373NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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