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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Campylobacter curvus 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP418154CWB
CSB-EP418154CWB
CSB-BP418154CWB
CSB-MP418154CWB
CSB-EP418154CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Large-conductance mechanosensitive channel (mscL), partial CSB-YP418155CWB1
CSB-EP418155CWB1
CSB-BP418155CWB1
CSB-MP418155CWB1
CSB-EP418155CWB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 30S ribosomal protein S6 (rpsF) CSB-YP418156CWB
CSB-EP418156CWB
CSB-BP418156CWB
CSB-MP418156CWB
CSB-EP418156CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Bifunctional protein GlmU (glmU) CSB-YP418157CWB
CSB-EP418157CWB
CSB-BP418157CWB
CSB-MP418157CWB
CSB-EP418157CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus tRNA modification GTPase MnmE (mnmE) CSB-YP418158CWB
CSB-EP418158CWB
CSB-BP418158CWB
CSB-MP418158CWB
CSB-EP418158CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L28 (rpmB) CSB-YP418159CWB
CSB-EP418159CWB
CSB-BP418159CWB
CSB-MP418159CWB
CSB-EP418159CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP418160CWB
CSB-EP418160CWB
CSB-BP418160CWB
CSB-MP418160CWB
CSB-EP418160CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP418161CWB
CSB-EP418161CWB
CSB-BP418161CWB
CSB-MP418161CWB
CSB-EP418161CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Ribosome maturation factor RimP (rimP) CSB-YP418162CWB
CSB-EP418162CWB
CSB-BP418162CWB
CSB-MP418162CWB
CSB-EP418162CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Histidinol-phosphate aminotransferase (hisC) CSB-YP418163CWB
CSB-EP418163CWB
CSB-BP418163CWB
CSB-MP418163CWB
CSB-EP418163CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Deoxycytidine triphosphate deaminase (dcd) CSB-YP418164CWB
CSB-EP418164CWB
CSB-BP418164CWB
CSB-MP418164CWB
CSB-EP418164CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus UPF0133 protein Ccur92_18190 (Ccur92_18190) CSB-YP418165CWB
CSB-EP418165CWB
CSB-BP418165CWB
CSB-MP418165CWB
CSB-EP418165CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L5 (rplE) CSB-YP418166CWB
CSB-EP418166CWB
CSB-BP418166CWB
CSB-MP418166CWB
CSB-EP418166CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus 50S ribosomal protein L4 (rplD) CSB-YP418167CWB
CSB-EP418167CWB
CSB-BP418167CWB
CSB-MP418167CWB
CSB-EP418167CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter curvus Orotate phosphoribosyltransferase (pyrE) CSB-YP418168CWB
CSB-EP418168CWB
CSB-BP418168CWB
CSB-MP418168CWB
CSB-EP418168CWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP418169CWE
CSB-EP418169CWE
CSB-BP418169CWE
CSB-MP418169CWE
CSB-EP418169CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Homoserine kinase (thrB) CSB-YP418170CWE
CSB-EP418170CWE
CSB-BP418170CWE
CSB-MP418170CWE
CSB-EP418170CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei UPF0059 membrane protein JJD26997_0180 (JJD26997_0180), partial CSB-YP418171CWE1
CSB-EP418171CWE1
CSB-BP418171CWE1
CSB-MP418171CWE1
CSB-EP418171CWE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Acetylglutamate kinase (argB) CSB-YP418172CWE
CSB-EP418172CWE
CSB-BP418172CWE
CSB-MP418172CWE
CSB-EP418172CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. doylei Non-canonical purine NTP pyrophosphatase (JJD26997_0276) CSB-YP418173CWE
CSB-EP418173CWE
CSB-BP418173CWE
CSB-MP418173CWE
CSB-EP418173CWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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