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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Cronobacter sakazakii Sulfurtransferase TusD (tusD) CSB-YP418414CXL
CSB-EP418414CXL
CSB-BP418414CXL
CSB-MP418414CXL
CSB-EP418414CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Glyoxylate/hydroxypyruvate reductase B (ghrB) CSB-YP418415CXL
CSB-EP418415CXL
CSB-BP418415CXL
CSB-MP418415CXL
CSB-EP418415CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Methylthioribose kinase (mtnK) CSB-YP418416CXL
CSB-EP418416CXL
CSB-BP418416CXL
CSB-MP418416CXL
CSB-EP418416CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein FdhD homolog (fdhD) CSB-YP418417CXL
CSB-EP418417CXL
CSB-BP418417CXL
CSB-MP418417CXL
CSB-EP418417CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Met repressor (metJ) CSB-YP418418CXL
CSB-EP418418CXL
CSB-BP418418CXL
CSB-MP418418CXL
CSB-EP418418CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Probable endonuclease 4 (nfo) CSB-YP418419CXL
CSB-EP418419CXL
CSB-BP418419CXL
CSB-MP418419CXL
CSB-EP418419CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0178 protein ESA_02916 (ESA_02916) CSB-YP418420CXL
CSB-EP418420CXL
CSB-BP418420CXL
CSB-MP418420CXL
CSB-EP418420CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP418421CXL
CSB-EP418421CXL
CSB-BP418421CXL
CSB-MP418421CXL
CSB-EP418421CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Protein hfq (hfq) CSB-YP418422CXL
CSB-EP418422CXL
CSB-BP418422CXL
CSB-MP418422CXL
CSB-EP418422CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0509 protein ESA_01586 (ESA_01586) CSB-YP418423CXL
CSB-EP418423CXL
CSB-BP418423CXL
CSB-MP418423CXL
CSB-EP418423CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Anhydro-N-acetylmuramic acid kinase (anmK) CSB-YP418424CXL
CSB-EP418424CXL
CSB-BP418424CXL
CSB-MP418424CXL
CSB-EP418424CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Electron transport complex protein RnfE (rnfE), partial CSB-YP418425CXL1
CSB-EP418425CXL1
CSB-BP418425CXL1
CSB-MP418425CXL1
CSB-EP418425CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii D-ribose pyranase (rbsD) CSB-YP418426CXL
CSB-EP418426CXL
CSB-BP418426CXL
CSB-MP418426CXL
CSB-EP418426CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii DNA replication and repair protein recF (recF) CSB-YP418427CXL
CSB-EP418427CXL
CSB-BP418427CXL
CSB-MP418427CXL
CSB-EP418427CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Pyrroloquinoline-quinone synthase (pqqC) CSB-YP418428CXL
CSB-EP418428CXL
CSB-BP418428CXL
CSB-MP418428CXL
CSB-EP418428CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0114 protein ESA_00283 (ESA_00283), partial CSB-YP418429CXL1
CSB-EP418429CXL1
CSB-BP418429CXL1
CSB-MP418429CXL1
CSB-EP418429CXL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii UPF0260 protein ESA_01462 (ESA_01462) CSB-YP418430CXL
CSB-EP418430CXL
CSB-BP418430CXL
CSB-MP418430CXL
CSB-EP418430CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Na (+)/H (+) antiporter nhaB, partial CSB-YP418431CXL
CSB-EP418431CXL
CSB-BP418431CXL
CSB-MP418431CXL
CSB-EP418431CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP418432CXL
CSB-EP418432CXL
CSB-BP418432CXL
CSB-MP418432CXL
CSB-EP418432CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cronobacter sakazakii N-succinylarginine dihydrolase (astB) CSB-YP418433CXL
CSB-EP418433CXL
CSB-BP418433CXL
CSB-MP418433CXL
CSB-EP418433CXL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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