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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Serratia proteamaculans Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP421516STJ
CSB-EP421516STJ
CSB-BP421516STJ
CSB-MP421516STJ
CSB-EP421516STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans GTPase obg (obg) CSB-YP421517STJ
CSB-EP421517STJ
CSB-BP421517STJ
CSB-MP421517STJ
CSB-EP421517STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S15 (rpsO) CSB-YP421518STJ
CSB-EP421518STJ
CSB-BP421518STJ
CSB-MP421518STJ
CSB-EP421518STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable cytosol aminopeptidase (pepA), partial CSB-YP421519STJ
CSB-EP421519STJ
CSB-BP421519STJ
CSB-MP421519STJ
CSB-EP421519STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 30S ribosomal protein S20 (rpsT) CSB-YP421520STJ
CSB-EP421520STJ
CSB-BP421520STJ
CSB-MP421520STJ
CSB-EP421520STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Dihydrodipicolinate reductase (dapB) CSB-YP421521STJ
CSB-EP421521STJ
CSB-BP421521STJ
CSB-MP421521STJ
CSB-EP421521STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP421522STJ
CSB-EP421522STJ
CSB-BP421522STJ
CSB-MP421522STJ
CSB-EP421522STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP421523STJ
CSB-EP421523STJ
CSB-BP421523STJ
CSB-MP421523STJ
CSB-EP421523STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Deoxyguanosinetriphosphate triphosphohydrolase (dgt), partial CSB-YP421524STJ
CSB-EP421524STJ
CSB-BP421524STJ
CSB-MP421524STJ
CSB-EP421524STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Siroheme synthase 1 (cysG1) CSB-YP421525STJ
CSB-EP421525STJ
CSB-BP421525STJ
CSB-MP421525STJ
CSB-EP421525STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP421526STJ
CSB-EP421526STJ
CSB-BP421526STJ
CSB-MP421526STJ
CSB-EP421526STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans S-ribosylhomocysteine lyase (luxS) CSB-YP421527STJ
CSB-EP421527STJ
CSB-BP421527STJ
CSB-MP421527STJ
CSB-EP421527STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Na (+)-translocating NADH-quinone reductase subunit F, partial CSB-YP421528STJ
CSB-EP421528STJ
CSB-BP421528STJ
CSB-MP421528STJ
CSB-EP421528STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Gamma-glutamyl phosphate reductase (proA) CSB-YP421529STJ
CSB-EP421529STJ
CSB-BP421529STJ
CSB-MP421529STJ
CSB-EP421529STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Recombination-associated protein rdgC (rdgC) CSB-YP421530STJ
CSB-EP421530STJ
CSB-BP421530STJ
CSB-MP421530STJ
CSB-EP421530STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP421531STJ
CSB-EP421531STJ
CSB-BP421531STJ
CSB-MP421531STJ
CSB-EP421531STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans 7-cyano-7-deazaguanine synthase (queC) CSB-YP421532STJ
CSB-EP421532STJ
CSB-BP421532STJ
CSB-MP421532STJ
CSB-EP421532STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans UPF0133 protein Spro_1136 (Spro_1136) CSB-YP421533STJ
CSB-EP421533STJ
CSB-BP421533STJ
CSB-MP421533STJ
CSB-EP421533STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Bifunctional protein FolD (folD) CSB-YP421534STJ
CSB-EP421534STJ
CSB-BP421534STJ
CSB-MP421534STJ
CSB-EP421534STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia proteamaculans Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP421535STJ
CSB-EP421535STJ
CSB-BP421535STJ
CSB-MP421535STJ
CSB-EP421535STJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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