Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Shikimate kinase 2 (aroL) CSB-YP454578YAW
CSB-EP454578YAW
CSB-BP454578YAW
CSB-MP454578YAW
CSB-EP454578YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Trigger factor (tig) CSB-YP454579YAW
CSB-EP454579YAW
CSB-BP454579YAW
CSB-MP454579YAW
CSB-EP454579YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Adenylate kinase (adk) CSB-YP454580YAW
CSB-EP454580YAW
CSB-BP454580YAW
CSB-MP454580YAW
CSB-EP454580YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L32 (rpmF) CSB-YP454581YAW
CSB-EP454581YAW
CSB-BP454581YAW
CSB-MP454581YAW
CSB-EP454581YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP454582YAW
CSB-EP454582YAW
CSB-BP454582YAW
CSB-MP454582YAW
CSB-EP454582YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Aspartate--ammonia ligase (asnA) CSB-YP454583YAW
CSB-EP454583YAW
CSB-BP454583YAW
CSB-MP454583YAW
CSB-EP454583YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Bifunctional protein FolD (folD) CSB-YP454584YAW
CSB-EP454584YAW
CSB-BP454584YAW
CSB-MP454584YAW
CSB-EP454584YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD), partial CSB-YP454585YAW
CSB-EP454585YAW
CSB-BP454585YAW
CSB-MP454585YAW
CSB-EP454585YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP454586YAW
CSB-EP454586YAW
CSB-BP454586YAW
CSB-MP454586YAW
CSB-EP454586YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Leucine--tRNA ligase (leuS), partial CSB-YP454587YAW
CSB-EP454587YAW
CSB-BP454587YAW
CSB-MP454587YAW
CSB-EP454587YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Protein grpE (grpE) CSB-YP454588YAW
CSB-EP454588YAW
CSB-BP454588YAW
CSB-MP454588YAW
CSB-EP454588YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Cell division protein ZipA homolog (zipA), partial CSB-YP454589YAW
CSB-EP454589YAW
CSB-BP454589YAW
CSB-MP454589YAW
CSB-EP454589YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Periplasmic nitrate reductase (napA), partial CSB-YP454590YAW
CSB-EP454590YAW
CSB-BP454590YAW
CSB-MP454590YAW
CSB-EP454590YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Ecotin (eco) CSB-YP454591YAW
CSB-EP454591YAW
CSB-BP454591YAW
CSB-MP454591YAW
CSB-EP454591YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 50S ribosomal protein L25 (rplY) CSB-YP454592YAW
CSB-EP454592YAW
CSB-BP454592YAW
CSB-MP454592YAW
CSB-EP454592YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Putative multidrug resistance protein MdtD (mdtD), partial CSB-YP454593YAW
CSB-EP454593YAW
CSB-BP454593YAW
CSB-MP454593YAW
CSB-EP454593YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Histidine--tRNA ligase (hisS) CSB-YP454594YAW
CSB-EP454594YAW
CSB-BP454594YAW
CSB-MP454594YAW
CSB-EP454594YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC CSB-YP454595YAW
CSB-EP454595YAW
CSB-BP454595YAW
CSB-MP454595YAW
CSB-EP454595YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB 3-deoxy-manno-octulosonate cytidylyltransferase (kdsB) CSB-YP454596YAW
CSB-EP454596YAW
CSB-BP454596YAW
CSB-MP454596YAW
CSB-EP454596YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB tRNA1 (Val) (adenine (37)-N6)-methyltransferase CSB-YP454597YAW
CSB-EP454597YAW
CSB-BP454597YAW
CSB-MP454597YAW
CSB-EP454597YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>