CLDN18
雙特異性抗體Claudin 18.2,抗腫瘤治療的“黑馬”靶點(diǎn)
Claudin-18 (CLDN18)是Claudin(CLDN)蛋白家族中的重要成員。Claudin是一類存在于上皮和內(nèi)皮緊密連接中的整合素膜蛋白,最早由Shoichiro Tsukita等于1998年發(fā)現(xiàn)。緊密連接,又稱閉鎖小帶,是相鄰細(xì)胞膜共同構(gòu)成的一個(gè)事實(shí)上液體無(wú)法穿透的屏障,多位于極化上皮細(xì)胞和內(nèi)皮細(xì)胞最頂端,是兩個(gè)細(xì)胞間緊密相連的區(qū)域。而Claudins是緊密連接的重要組成部分。幾乎所有的脊椎動(dòng)物都有Claudin蛋白。大多數(shù)物種只種Claudin,但人類有除了Claudin 13之外,從Claudin 1到Claudin 27的26種Claudin。
CLDN18是肺和胃上皮細(xì)胞緊密連接(tight junction)的一個(gè)主要成分,有兩個(gè)異構(gòu)體,分別是Claudin18.1(CLDN18.1)和Claudin18.2(CLDN18.2)。CLDN18.1主要在肺部表達(dá),CLDN18.2主要在胃上皮細(xì)胞中表達(dá),而且,CLDN18.2只在胃粘膜上分化的上皮細(xì)胞中表達(dá),正常情況下是不表達(dá)于其他任何健康組織。然而,它在原發(fā)性惡性腫瘤中高度表達(dá),如胃癌、乳腺癌、結(jié)腸癌和肝癌。CLDN18.1和CLDN18.2都由261個(gè)氨基酸組成,有四個(gè)跨膜結(jié)構(gòu)域(Figure 2)和兩個(gè)胞外環(huán)(胞外環(huán):ECL1,ECL2),其NH2和COOH端均位于細(xì)胞內(nèi)。CLDN18.1和CLDN18.2僅在ECL1大約50個(gè)氨基酸的序列中有7個(gè)氨基酸殘基差異。
目前,CLDN18.2是研究得最為清楚的Claudin家族蛋白。在正常組織中,Claudin的表達(dá)具有高度組織特異性,僅在埋藏于胃粘膜上已分化的上皮細(xì)胞中表達(dá),而且正常組織中的單克隆抗體基本上檢測(cè)不到。但是,惡性腫瘤的發(fā)生會(huì)導(dǎo)致緊密連接的破壞,使腫瘤細(xì)胞表面的CLDN18.2表位暴露出來(lái),成為特定的靶點(diǎn)。
CUSABIO-哺乳包膜VIPs平臺(tái)成功開(kāi)發(fā)CLDN18.2雙特異性受體(4次跨膜),展示了完整生物活性。
熱銷產(chǎn)品
● Recombinant Human Claudin-18.2(CLDN18.2)-VLPs (Active) (CSB-MP005498HU(A5))
驗(yàn)證數(shù)據(jù)
Immobilized human CLDN18.2 at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC50 is 5.225-9.256 ng/ml.
The presence of VLP-like structures was confirmed by TEM
● Claudin-18.2 Monoclonal Antibody (ELISA, FC) (CSB-RA005498A1HU)
驗(yàn)證數(shù)據(jù)
Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/mL can bind Anti-CLDN18.2 recombinant antibody, the EC50 is 6.554-27.87 ng/mL.
Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18.2 antibody (2μg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with FC.
CLDN18 Antibodies
CLDN18 for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 種屬反應(yīng)性 | 應(yīng)用類型 |
---|---|---|---|
CSB-PA005498GA01HU | CLDN18 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
CSB-PA005498ESR1HU | CLDN18 Antibody | Human | ELISA, WB, IHC |
CSB-PA005498ESR2HU | CLDN18 Antibody | Human, Rat | ELISA, WB, IHC |
CSB-MA148581 | CLDN18 Monoclonal Antibody | Human | ELISA, IHC |
CSB-PA005498LA01HU | CLDN18 Antibody | Human | ELISA, IHC, IF |
CSB-PA005498LC01HU | CLDN18 Antibody, FITC conjugated | Human | |
CSB-PA005498LB01HU | CLDN18 Antibody, HRP conjugated | Human | ELISA |
CSB-RA005498A2HU | CLDN18 Recombinant Monoclonal Antibody | Human | ELISA, FC |
CLDN18 Proteins
CLDN18 Proteins for Mus musculus (Mouse)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
---|---|---|
CSB-YP005498MO1 CSB-EP005498MO1 CSB-BP005498MO1 CSB-MP005498MO1 CSB-EP005498MO1-B |
Recombinant Mouse Claudin-18 (Cldn18), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF005498MO | Recombinant Mouse Claudin-18 (Cldn18) | in vitro E.coli expression system |
CLDN18 Proteins for Homo sapiens (Human)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
---|---|---|
CSB-YP005498HU1 CSB-EP005498HU1 CSB-BP005498HU1 CSB-MP005498HU1 CSB-EP005498HU1-B |
Recombinant Human Claudin-18 (CLDN18), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF005498HU | Recombinant Human Claudin-18 (CLDN18) | in vitro E.coli expression system |
CSB-MP005498HU(A5) | Recombinant Human Claudin-18.2 (CLDN18.2)-VLPs (Active) | Mammalian cell |
CSB-EP005498HU2 | Recombinant Human Claudin-18 (CLDN18), partial | E.coli |
CLDN18 Proteins for Bos taurus (Bovine)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱 | 來(lái)源 |
---|---|---|
CSB-YP610823BO1 CSB-EP610823BO1 CSB-BP610823BO1 CSB-MP610823BO1 CSB-EP610823BO1-B |
Recombinant Bovine Claudin-18 (CLDN18), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-CF610823BO | Recombinant Bovine Claudin-18 (CLDN18) | in vitro E.coli expression system |