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EPHA3

EphA3:細胞粘附和遷移的調(diào)節(jié)器

EphA3屬于Eph受體家族的一員,被認為是細胞粘附和遷移的調(diào)節(jié)器。EphA3最初是從前B淋巴白血病細胞表面(ALL)細胞系(LK63)中分離得到的表面抗原。EphA3在大腦和脊髓,肺,腎,心臟和肌肉組織的胚胎發(fā)育的各個階段高度表達。在正常成人組織中EphA3幾乎不存在,并且在中樞神經(jīng)系統(tǒng)(CNS)中稀疏表達,在視網(wǎng)膜中表達相對較高,在膀胱,前列腺,子宮和心臟中的表達較少。EphA3在胃癌、肺癌、腎癌、結(jié)腸癌、黑色素瘤、肉瘤、膽管癌、前列腺癌等實體腫瘤中異常表達,并且與腫瘤的侵襲轉(zhuǎn)移相關。EphA3的配體包括Ephrin-B2和Ephrin-A5。EphA3與配體結(jié)合,在正常細胞黏附、遷移、血管生成中發(fā)揮重要的作用,研究表明Eph受體或ephrin蛋白在維持血管、腎臟及腸道等組織的結(jié)構(gòu)上發(fā)揮著一定的作用。此外,它們還和許多腫瘤血管的形成密切相關,并被認為參與腫瘤的侵襲和轉(zhuǎn)移。

Recombinant Human EPHA3 活性蛋白實驗驗證數(shù)據(jù)

High Purity Validated by
SDS-PAGE

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized EPHA3 at 2 μg/ml can bind human EFNA5(CSB-MP007464HU), the EC50 of the protein is 0.9734-1.179 ng/ml.

Excellent Bioactivity Validated by LSPR Assay

Human EPHA3 protein his tag (CSB-MP007723HU) captured on COOH chip can bind Human EFNA5 protein Fc tag (CSB-MP007464HU) with an affinity constant of 13.8 nM as detected by LSPR Assay.

 

EPHA3 Antibodies

EPHA3 for Homo sapiens (Human)

EPHA3 Proteins

EPHA3 Proteins for Homo sapiens (Human)

EPHA3 Proteins for Mus musculus (Mouse)

EPHA3 Proteins for Gallus gallus (Chicken)

EPHA3 Proteins for Rattus norvegicus (Rat)

EPHA3 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)