YTFE
YTFE,即二鐵蛋白YtfE,別名RCMNS。這是一種在大腸桿菌中發(fā)現(xiàn)的蛋白質(zhì),屬于二鐵蛋白家族。YtfE的作用機(jī)制涉及其雙鐵位點(diǎn),當(dāng)亞硝酸鹽添加到二亞鐵YtfE中時(shí),會(huì)導(dǎo)致亞硝基化YtfE并釋放NO。YtfE還表現(xiàn)出非常低的NO還原酶活性,并且只能在損壞其雙鐵中心的條件下充當(dāng)重建脫輔基鐵氧還蛋白的鐵供體。YtfE是一種高親和力、低容量的亞硝酸還原酶,通過(guò)與共同調(diào)節(jié)的NO消耗酶Hmp和Hcp聯(lián)合作用來(lái)緩解亞硝化應(yīng)激。YtfE在大腸桿菌中的表達(dá)在亞硝化應(yīng)激條件下受到高度誘導(dǎo),有助于細(xì)胞在這種應(yīng)激條件下的生存。此外,YtfE還與饑餓細(xì)胞(Dps)的DNA結(jié)合蛋白相互作用,以減少由RIC蛋白介導(dǎo)的氧化還原應(yīng)激。這些功能表明YtfE在細(xì)菌對(duì)亞硝化應(yīng)激的響應(yīng)中扮演著重要角色。
熱銷(xiāo)產(chǎn)品
驗(yàn)證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized aqpZ at 5 μg/ml can bind E.coli ytfE, the EC50 of E.coli ytfE protein is 197.90-259.70 μg/ml.
YTFE Proteins
YTFE Proteins for Escherichia coli (strain K12)
產(chǎn)品貨號(hào) | 產(chǎn)品名稱(chēng) | 來(lái)源 |
---|---|---|
CSB-YP303998ENV CSB-BP303998ENV CSB-MP303998ENV CSB-EP303998ENV-B |
Recombinant Escherichia coli Iron-sulfur cluster repair protein YtfE (ytfE) | Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP303998ENV | Recombinant Escherichia coli Iron-sulfur cluster repair protein ytfE (ytfE) (Active) | E.coli |