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  4. Bovine growth hormone,GH ELISA Kit

Bovine growth hormone,GH ELISA Kit

  • 中文名稱：
    牛生長激素 (GH)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E13443B
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Bovine GH1 ELISA Kit was designed for the quantitative measurement of Bovine GH1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.25 ng/mL-50 ng/mL and the sensitivity is 1.25 ng/mL.

  • 別名：
    GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Bos taurus (Bovine)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.25 ng/mL-50 ng/mL
  • 靈敏度：
    1.25 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 101  
    Range % 98-105  
    1:2 Average % 85  
    Range % 81-89  
    1:4 Average % 87  
    Range % 82-90  
    1:8 Average % 93  
    Range % 90-96  
  • 回收率：
    The recovery of bovine GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-99  
    EDTA plasma (n=4) 92 87-96  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    50 0.332 0.331 0.332    
    25 0.538 0.508 0.523    
    11.5 0.768 0.735 0.752    
    3.75 1.086 1.099 1.093    
    1.25 1.632 1.657 1.645    
    0 2.131 2.168 2.150    
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

問答及客戶評論

■ 常見問題解答
Q:

Please provide the following information regarding Bovine growth hormone, GH ELISA Kit (CSB-E13443B)
a. What is the standard?
b. What is the antibody in this kit?

A:
Thanks for your inquiry.
CSB-E13443B
Standard：293 cell-derived recombinant protein；
The plate is coated with goat anti-rabbit secondary antibody, and the specific antibody is rabbit polyclonal antibody.
Pls let me know if you have any further questions. Thank you.

靶點詳情

  • 功能：
    Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 基因功能參考文獻：
    1. Tracing Recombinant Bovine Somatotropin Ab(Use) Through Gene Expression in Blood, Hair Follicles, and Milk Somatic Cells. PMID: 30011805
    2. A microarray analysis showed that lncYYW positively regulates the expression of growth hormone 1 and its downstream genes, AKT1 and PIK3CD, in bovine myoblasts. This discovery provides a good foundation for further study of the mechanism of action of lncYYW during bovine myoblast development PMID: 28726188
    3. bGH mice had increased body weight and decreased percent fat mass. Serum FGF21 levels were significantly elevated in bGH mice. Expression of Fgf21, Fgfr1, and Klb mRNA in white AT and liver were downregulated or unchanged in bGH mice. PMID: 27585733
    4. GH/HpaII locus as candidate marker for body weight in cattle rather than MSTN/DraI. PMID: 29023020
    5. An SNP GH4.1 in a growth hormone gene was significantly related to a weaning weight. PMID: 27706683
    6. The association of IGF1, GH, and PIT1 markers with growth and reproductive traits were assessed. PMID: 25258122
    7. Male bovine GH transgenic mice have decreased adiposity, and have increased regulatory T cells and macrophages in mesenteric and subcutaneous white adipose tissue. PMID: 25521584
    8. The total frequencies for the combined genotypes for the bGH and RORC genes, which provide for superior meat quality and carcass weight, in the populations of Kazakh white-headed cattle PMID: 25975152
    9. There is an association between a polymorphism in intron 3 of the BGH gene and both body weight at first estrus and first calving. PMID: 25158245
    10. Whereas only moderate structural changes were observed with up-regulation of GH/IGF-I axis, disruption of the GH receptor had pronounced effects upon tendon ultra-structure. PMID: 24080228
    11. this study identifies a biochemical mechanism for the regulation of SCFAs on bovine GH and PRL gene transcription in dairy cow anterior pituitary cells PMID: 24177567
    12. homozygote genotypes of GH (LL) and beta-LG (AA) were superior compared to heterozygote genotypes, whereas, the heterozygote genotype of Pit-1 gene (AB) was desirable PMID: 22730770
    13. one GH1 SNP, GH33, was significantly associated with milk protein yield in the second lactation. Several GH1 SNPs were significantly associated with fertility. PMID: 21948746
    14. GH and IGF-I genotypes had no substantial effect on productive parameters, though IGF-I genotype affected calving-first service interval in primiparous cows. However, the genotypes do alter the endocrine/metabolic profiles of the transition dairy cow. PMID: 21635772
    15. Association of cattle growth hormone gene polymorphism with milk productivity PMID: 21866862
    16. Leptin modulation of lymphocytic growth hormone plays a role in the regulation of immune response during pregnancy. PMID: 21081139
    17. Results further demonstrate the multifaceted influences of GH1 on milk production, fertility, and growth-related traits in cattle. PMID: 21094770
    18. Study of genetic variation in Yakutian cattle (Bos taurus L.) using the prolactin bPRL, growth hormone bGH, and transcription factor bPit-1 genes PMID: 20391788
    19. More than half of the CSF GHRH pulses observed in the current study were followed by episodes of GH release; leptin suppressed, while neuropeptide Y increased serum GH levels. PMID: 19632078
    20. Findings suggest that the GH gene polymorphism at codons 127 and 172 was related to body weight, circulating basal GH and insulin, and GHRH-induced GH responses in Japanese Black heifers. PMID: 19747793
    21. bGH-transgenic mice display marked changes in hepatic genes coding for metabolic enzymes and suggest that GH directly or indirectly regulates many of these hepatic genes via decreased expression of PPARalpha and SREBP-1. PMID: 12736163
    22. GH administration had no effect on plasma leptin during late pregnancy or early lactation. PMID: 12881203
    23. Hepatic growth hormone receptor(GHR) and IGF-I mRNA levels were strongly correlated with each other in chronic GH excess or deficiency. Regulation and correlation between GHR and IGF-I mRNA levels induced by GH is different between liver and pituitary. PMID: 14726438
    24. Associations were analysed between polymorphisms of the growth hormone gene (GH-MspI) (localized in intron 3) and milk production traits of Beijing Holstein cows PMID: 16388132
    25. results indicate that serine 84 is crucial for the activity of bGH despite this site being distal to the receptor binding surfaces PMID: 16514176
    26. GH dramatically inhibits the expression of IGFBP-5, and GH along with IGF-I enhanced the phosphorylation of Akt through the reduction of IGF binding protein (IGFBP)-5. PMID: 16698222
    27. insulin-regulated resensitization of GH signaling may be necessary to reset the complete response to GH after a normal, physiologic pulse of GH PMID: 16717097
    28. A missense mutation within the GH1 gene is associated with body fat distribution. PMID: 16734678
    29. GH, IGF-I and IGF-IBP3 regulated the growth and development traits in different growth period. PMID: 16870578
    30. Characterization of single nucleotide polymorphisms in GH1 in relation to production traits. PMID: 16879354
    31. nitration of JAK2 may act as an inhibitory counterpart to phosphorylation activation, reflecting a very localized break on the progression of GH signal transduction processes spanning JAK-STAT-AKT interactions PMID: 17510232
    32. Gh(deficient (dr/dr) rats, which are nearly refractory to mammary carcinogenesis, can be made vulnerable by restoring GH and IGF-I. Advanced rat mammary cancers are dependent on GH and/or IGF-I for their survival. PMID: 17584969
    33. Transmission ratio distortion at the growth hormone gene (GH1) in bovine preimplantation embryos PMID: 17948248
    34. overexpression of growth hormone is associated with kidney diseases PMID: 18097769
    35. Peak-shaped responses were observed for concentrations of LH and GH, and the peaks were observed at 27+/-3 and 75+/-9 min, respectively, after injection, only in heifers injected with kisspeptin-10. PMID: 18252956
    36. 940 bGH haplotypes were compared with respect to six carcass traits: carcass weight, longissimus muscle area, rib thickness, subcutaneous fat thickness, beef marbling score and beef colour. PMID: 18254825
    37. Daytime and nighttime profiles of growth hormone(GH) secretion and the effect of a photic stimulation on nocturnal GH and melatonin secretion were investigated in Holstein steers. PMID: 18375661
    38. GH expression (bGH) and its receptor binding (GHR-/-) had profound and divergent effects on muscle phenotype. PMID: 18499495
    39. Age-related changes in body composition of bovine growth hormone transgenic mice. PMID: 18948397
    40. AluI polymorphism of the growth hormone gene had no effect on the interval from calving to first ovulation and could not be directly related to differences in milk yield and to the extent of body condition loss during the first month after calving. PMID: 19111333
    41. Chronic GH excess increased FA metabolism in the young age, whereas its action was overwhelmed in elder ages likely by GH-independent mechanisms. PMID: 19342398

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Somatotropin/prolactin family
  • 數(shù)據(jù)庫鏈接：