Human Collagen Type Ⅱ,Col Ⅱ ELISA Kit
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中文名稱:人Ⅱ型膠原(Col Ⅱ)酶聯(lián)免疫試劑盒
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貨號:CSB-E08087h
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規(guī)格:96T/48T
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價(jià)格:¥3600/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:
This Human Col Ⅱ ELISA Kit was designed for the quantitative measurement of Human Col Ⅱ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
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別名:Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
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縮寫:Col Ⅱ
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:31.25 pg/mL-2000 pg/mL
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靈敏度:7.8 pg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 86 Range % 80-90 1:200 Average % 93 Range % 88-98 1:400 Average % 94 Range % 90-100 1:800 Average % 96 Range % 90-102 -
回收率:
The recovery of human Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 88-98 EDTA plasma (n=4) 95 90-100 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 2000 2.478 2.387 2.433 2.341 1000 1.811 1.752 1.782 1.690 500 1.237 1.142 1.190 1.098 250 0.863 0.813 0.838 0.746 125 0.398 0.374 0.386 0.294 62.5 0.211 0.198 0.205 0.113 31.25 0.145 0.141 0.143 0.051 0 0.093 0.090 0.092 -
數(shù)據(jù)處理:
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貨期:3-5 working days
引用文獻(xiàn)
- Pd@ Pt nanoparticle-linked immunosorbent assay for quantification of Collagen type II EY Kwon,Analytica chimica acta,2023
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I. Frisch J.et al,Tissue Eng Part A. ,2015
- Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Frisch J. et al,Tissue Eng Part A. ,2015
- Relation of thyroid hormone abnormalities with subclinical inflammatory activity in patients with type 1 and type 2 diabetes mellitus Moura Neto A. et al,Endocrine. ,2015
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