Your Good Partner in Biology Research

  1. 首頁
  2. 試劑盒
  3. 酶聯(lián)免疫試劑盒
  4. Human SARS-CoV-2 N IgG Antibody ELISA Kit

Human SARS-CoV-2 N IgG Antibody ELISA Kit

  • 中文名稱：
    人新型冠狀病毒核蛋白(SARS-CoV-2 N) IgG抗體酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-EL3325HU
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 其他：

產品詳情

  • 產品描述：

    CUSABIO's Human SARS-CoV-2 nucleoprotein (N) IgG Antibody ELISA Kit allows for in vitro qualitative detection of the IgG antibody-specific for SARS-CoV-2 N protein in human serum and plasma. This research-use only kit can be applied in the studies associated with COVID-19, infectious and severe pneumonia caused by SARS-CoV-2. It has been validated with high sensitivity, high specificity, and high precision.

    Nucleoprotein is the most abundant protein in SARS-CoV-2. It packs the positive-sense single-stranded viral RNA into a helical ribonucleocapsid (RNP), forming the viral nucleocapsid (N). The N protein plays an important role in improving the efficiency of virus transcription and assembly. It is also involved in viral pathogenesis. N protein possessing an immunogenic effect makes it trigger an immune response, which leads to the production of IgM/G against SARA-CoV-2 N protein. SARS-CoV-2 N IgG antibodies appear in the serum after 2-3 weeks after the onset of the symptoms, peaks within the third week, and gradually declines to a lower level. IgG-specific for SARS-CoV-2 N protein rapidly increases to a higher level after a secondary infection and retains in the body for several months even years.

  • 別名：
    N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
  • 縮寫：
    SARS-CoV-2 N Ab (IgG)
  • Uniprot No.：
  • 種屬：
    Homo sapiens (Human)
  • 樣本類型：
    serum, plasma
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Infectious Diseases
  • 測定原理：
    qualitative
  • 測定方法：
    Indirect
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%  
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%  
    Three samples of known concentration were tested in twenty assays to assess.
  • 標準曲線：
    Test parameter specification test result
    Positive control ≥0.6 0.867
    Negative control ≤0.25 0.184
    Positive rate 20，Positive 100%
    Negative rate 20，Negative 100%
  • 本試劑盒所含材料：
      • A 96-well Coated assay plate --This microplate has been pre-coated with human SARS-CoV-2 nucleoprotein.
      • Negative Control (1 x 800 μl) --It is free of the SARS-CoV-2 N IgG antibody and used to preclude the false positive.
      • Positive Control (1 x 800 μl) --Used to evaluate the validity, stability, and comparability of the test results.
      • HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) --Act as the detection antibody.
      • HRP-conjugate Diluent (1 x 20 ml) --Dilute the HRP-conjugated anti-Human IgG antibody.
      • Sample Diluent (2 x 20 ml) --Dilute the sample solution.
      • Wash Buffer (25 x concentrate) (1 x 20 ml) --Wash the unbound reagents.
      • TMB Substrate (1 x 10 ml) --React with HRP, eliciting a chromogenic color reaction.
      • Stop Solution (1 x 10 ml) --Stop the color reaction. The solution turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells) --Seal the microplate when incubation.
      • An Instruction manual

    顯示更多

    收起更多

  • 本試劑盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°C.
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer.
      • Absorbent paper for blotting the microtiter plate.
      • 50-300ul multi-channel micropipette
      • 100ml and 500ml graduated cylinders.
      • Deionized or distilled water.
      • Pipette tips
      • Timer
      • Test tubes for dilution.

    顯示更多

    收起更多

  • 貨期：
    3-5 working days

產品評價

問答及客戶評論

■ 常見問題解答
Q:

如何使用檢測核蛋白或刺突蛋白的Elisa試劑盒？推薦的使用目的有什么不同嗎？另外，您建議對CoVID-19檢測是用IgG還是IgM？

A:
關于檢測CoVID-19的ELISA試劑盒，我們只能提供以下產品:CSB-EL3325HU， Human Novel Coronavirus Nucleoprotein (SARS-CoV-2 N) IgG Antibody ELISA Kit。它只用于科研，不用于臨床或診斷。通常，IgM首先在原發(fā)性免疫反應中產生。IgM首先出現(xiàn)在病毒感染過程中，但持續(xù)時間不長；IgG是再免疫反應產生的主要抗體，持續(xù)時間較長，表明過去有感染。

靶點詳情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
  • 亞細胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Betacoronavirus nucleocapsid protein family