The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 201.1 ng/ml.
Western Blot Positive WB detected in: SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY1) at 100ng All lanes N antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 18 KDa Observed band size: 18 KDa
CUSABIO uses the full-length amino acid sequence of the recombinant human SARS-CoV-2 nucleoprotein (N) to immunize rabbits thus obtaining the N antibody (CSB-PA33254A0Rb). This N antibody is a polyclonal antibody and occurs as an unconjugated IgG. Its purity is greater than 95% using protein G purified. It only reacts with human SARS-CoV-2 N protein. It has been validated for use in ELISA and WB applications. In the functional ELISA, this N antibody can bind to the SARS-CoV-2 N protein, and its EC50 is 201.1 ng/ml. The target protein SARS-CoV-2 N is highly expressed within infected cells and is essential for viral RNA replication and assembling into new virions.
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Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.