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中文名稱:小鼠胰島素樣生長因子結合蛋白2(IGFBP-2)酶聯(lián)免疫試劑盒
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貨號:CSB-E04589m
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規(guī)格:96T/48T
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價格:¥3200/¥2500
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其他:
產品詳情
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產品描述:
This Mouse IGFBP2 ELISA Kit was designed for the quantitative measurement of Mouse IGFBP2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.45 ng/mL-30 ng/mL and the sensitivity is 0.11 ng/mL.
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別名:Igfbp2 ELISA Kit; Igfbp-2Insulin-like growth factor-binding protein 2 ELISA Kit; IBP-2 ELISA Kit; IGF-binding protein 2 ELISA Kit; IGFBP-2 ELISA Kit; mIGFBP-2 ELISA Kit
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.45 ng/mL-30 ng/mL
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靈敏度:0.11 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IGFBP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 103 Range % 98-107 1:40 Average % 84 Range % 80-88 1:80 Average % 96 Range % 92-100 1:160 Average % 90 Range % 86-94 -
回收率:
The recovery of mouse IGFBP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 84-92 EDTA plasma (n=4) 95 90-100 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 30 2.641 2.496 2.569 2.470 15 1.750 1.625 1.688 1.589 7.5 1.004 1.080 1.042 0.943 3.75 0.638 0.659 0.649 0.550 1.8 0.329 0.313 0.321 0.222 0.9 0.215 0.204 0.210 0.111 0.45 0.162 0.171 0.167 0.068 0 0.096 0.102 0.099 -
數(shù)據處理:
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貨期:3-5 working days
引用文獻
- Icaritin-curcumol activates CD8+ T cells through regulation of gut microbiota and the DNMT1/IGFBP2 axis to suppress the development of prostate cancer W Xu,Journal of experimental & clinical cancer research,2024
相關產品
靶點詳情
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功能:Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
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基因功能參考文獻:
- AAV6-mediated IGFBP-2 overexpression in the tibialis anterior (TA) muscles of 4-week-old C57BL/10 and mdx mice reduced the mass of injected muscle after 8weeks, inducing a slower muscle phenotype in C57BL/10 but not mdx mice. Analysis of inflammatory and fibrotic gene expression revealed no changes between control and IGFBP-2 injected muscles in dystrophic (mdx) mice. PMID: 27544574
- Peptides that disrupted p62/PKCzeta or p62/IRS-1 inhibited IGF-I/IGFBP-2 stimulated PKCzeta activation, vimentin phosphorylation, PTEN tyrosine phosphorylation, AKT activation, and osteoblast differentiation. PMID: 26773517
- In human whole blood cells, methylation of IGFBP2 at the homologous CpG site was increased in obese men with impaired glucose tolerance. In conclusion, our data show that increased methylation of hepatic Igfbp2 during infancy predicts the development of fatty liver later in life and is linked to deterioration of glucose metabolism. PMID: 27126637
- IGFBP2 not only is a driver of glioma progression and a prognostic factor but is also required for tumour maintenance and thus represents a viable therapeutic target in the treatment of glioma. PMID: 27125842
- that the lacking the Arg-Gly-Asp-domain has a major influence on the pleiotropic effects of IGFBP-2 and suggest that somatic growth and time of sexual maturity or somatic growth and life expectancy are less closely related than thought previously PMID: 26507795
- Data (including data from studies in knockout mice) suggest, as osteoblasts differentiate, early induction of AMPK in response to IGF-I/IGFBP-2 is followed by suppression of AMPK as required for osteogenesis; activation of AMPK stimulates autophagy. PMID: 26556533
- estrogen deficiency has a profound effect on body and bone composition in the absence of IGFBP-2 and may be related to changes in fibroblast growth factor 21. PMID: 26230658
- Impaired glucose clearance in female IGFBP-2 transgenic mice is dependent on the presence of the RGD motif and that translocation of GLUT4 in the muscle may be regulated by IGFBP-2 via RGD-dependent mechanisms. PMID: 26105006
- protein(s) that associated with RPTPbeta in response to IGF-I and IGFBP-2 in vascular smooth muscle cells PMID: 25787077
- The results suggest that stimulation of differentiation is an important mechanism by which IGFBP-2 regulates the acquisition of normal bone mass in mice. PMID: 24839202
- PPARalpha controls IGF-1 signalling through the up-regulation of hepatic Igfbp-2 transcription during fasting. PMID: 25695641
- IGFBP2 is a critical cell-autonomous factor that promotes the survival and migration of acute leukemia cells. PMID: 24191913
- IGFBP-2 plays an IGF-I-dependent and -independent role in the brain's acute (neuroprotection) and chronic (tissue remodeling) response to hypoxic-ischemic injury. PMID: 24359611
- physiological levels of IGFBP2 are neither sufficient to mimic nor required for the physiological action of leptin PMID: 24424049
- Igfbp2-5 are expressed in distinct and complementary patterns during cochlear development. PMID: 23813480
- These findings demonstrate that the HBD2 domain of IGFBP-2 is the primary region that accounts for its ability to inhibit adipogenesis and that a peptide encompassing this region has activity that is comparable with native IGFBP-2. PMID: 23981772
- we have identified a single polymorphic locus that affects skin and lung tumorigenesis and identify Igfbp5 and Igfbp2 as candidate modifier genes of lung tumorigenesis. PMID: 22973541
- IGFBP-2 bound receptor protein tyrosine phosphatase beta, which led to its dimerization and inactivation. Analysis of aortas obtained from IGFBP-2(-/-) mice showed that receptor protein tyrosine phosphatase beta was activated. PMID: 22869525
- findings suggest that insulin upregulates IGFBP-2 expression through a PI3K/mTOR/C/EBP-alpha pathway in white adipocytes PMID: 22410287
- Data from transgenic mice suggest that hepatic leptin signaling (i.e., direct action of leptin on hepatocytes) is not required for leptin up-regulation of plasma levels of IGFBP-2; intact vagus nerve is also not required. PMID: 22202803
- IGFBP-2 is an important regulator of osteoclastogenesis. PMID: 22006816
- IGF binding protein 2 supports the survival and cycling of hematopoietic stem cells. PMID: 21821709
- the HBD peptide of IGFBP-2 has anabolic activity by activating IGF-I/Akt and beta-catenin signaling pathways PMID: 21372140
- The present study was aimed at determining IGFBP2 mRNA levels in adipose tissue in conditions of insulin resistance such as aging and obesity. PMID: 20730703
- We propose that Igfbp2 mediates epidermal progenitor cell expansion and therefore represents an epidermal progenitor cell-specific target of Hedgehog signaling that promotes basal cell carcinoma development PMID: 20858761
- IGFBP-2 significantly inhibits postnatal skeletal myofiber growth by decreasing myogenic proliferation and protein accretion and enhances glycolytic muscle metabolism PMID: 20501873
- Overexpression of IGFBP2 by an adenovirus reversed diabetes in insulin-resistant and diet-induced obese mice, as well as insulin-deficient streptozotocin-treated mice. PMID: 20074524
- Data show that IGFBP-2 potently separates hypertrophic and hyperplastic effects of GH/IGF-I excess on adrenocortical cells. PMID: 12409314
- During cell differentiation, secretion is controlled by hormones in mouse mammary epithelial cells. PMID: 12914536
- effect of IGFBP-2 overexpression on the activity of various antioxidative enzymes in two malignant cell lines PMID: 14710363
- menin regulates the expression of the endogenous IGFBP-2 gene at least in part through the promoter of IGFBP-2. PMID: 15044367
- intact insulin-like growth factor binding protein-2 and a distinct carboxyl-terminal IGFBP-2 fragment localize to the cell nucleus PMID: 15474485
- IGFBP2 expression is responsible for increased insulin receptor substrate-1 phosphorylation in mesangial cells from mice susceptible to glomerulosclerosis PMID: 16556765
- IGFBP-2 may regulate bone turnover in both an IGF-I-dependent and -independent manner. PMID: 18276763
- IGF axis as key mechanisms underlying nephropathic responses of mesangial cells to Ang II and high glucose. PMID: 18392786
- Results suggest that the growth-promoting role of IGFBP-2 in prostate cancer is inhibited by its intracellular interaction with PAPA-1. PMID: 19095771
- IGFBP-2 overexpression reduces the appearance of dysplastic aberrant crypt foci and inhibits growth of adenomas in chemically induced colorectal carcinogenesis PMID: 19142966
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亞細胞定位:Secreted.
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組織特異性:Highly expressed in adult liver, but also in kidney, lung, brain, spleen, testis and ovary.
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