Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT
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中文名稱:小鼠腫瘤壞死因子可溶性受體Ⅱ(TNFsR-Ⅱ)酶聯(lián)免疫試劑盒
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貨號:CSB-E04739m
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規(guī)格:96T/48T
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價格:¥3800/¥2500
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其他:
產(chǎn)品詳情
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產(chǎn)品描述:TNFRSF1B,全名為腫瘤壞死因子受體家族成員 1B(TNF receptor superfamily member 1B),也稱為TNFR2。TNFR2的分布比TNFR1更為局限,主要存在于免疫細胞表面,其信號通路與TNFR1類似,但與細胞增殖和凋亡的調(diào)節(jié)有關(guān)的分子和通路不同。TNFR2的調(diào)節(jié)和作用機制與TNFR1存在著復(fù)雜的相互影響和調(diào)節(jié)。華美生物所提供的Mouse Tumor necrosis factor soluble receptor Ⅱ,TNFsR-Ⅱ ELISA KIT屬于ELISA檢測試劑盒,采用雙抗夾心法定量檢測鼠血清、血漿、組織勻漿樣本中的TNFRSF1B,其靈敏度為1.6 pg/mL,檢測范圍為7.8125 pg/mL-500 pg/mL。
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別名:Tnfrsf1b; Tnfr-2; Tnfr2; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; TNF-RII; TNFR-II; p75; p80 TNF-alpha receptor; CD antigen CD120b
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縮寫:
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Uniprot No.:
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種屬:Mus musculus (Mouse)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:7.8125 pg/mL-500 pg/mL
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靈敏度:1.6 pg/mL
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反應(yīng)時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Signal Transduction
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFsR-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:20 Average % 91 Range % 86-95 1:40 Average % 97 Range % 92-102 1:80 Average % 90 Range % 87-98 1:160 Average % 92 Range % 85-96 -
回收率:
The recovery of mouse TNFsR-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 89-97 EDTA plasma (n=4) 98 95-101 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 500 2.858 2.911 2.885 2.777 250 2.360 2.250 2.305 2.197 125 1.485 1.455 1.470 1.362 62.5 0.785 0.812 0.799 0.691 31.25 0.482 0.521 0.502 0.394 15.625 0.270 0.263 0.267 0.159 7.8125 0.189 0.187 0.188 0.080 0 0.104 0.112 0.108 -
數(shù)據(jù)處理:
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貨期:3-5 working days
相關(guān)產(chǎn)品
靶點詳情
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最新研究進展:TNFRSF1B又稱為腫瘤壞死因子受體2(TNF receptor 2),它可以結(jié)合腫瘤壞死因子(TNF)并轉(zhuǎn)導(dǎo)信號,參與炎癥、細胞增殖和凋亡等生物學(xué)過程。近年來的研究表明,TNFRSF1B在腫瘤的免疫治療中起到了重要作用,研究人員正在探索利用TNFRSF1B作為免疫治療靶點的可能性。
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功能:Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2.
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基因功能參考文獻:
- MPTP induced TNF-alpha signaling through TNFR2 mediated pathway and recruited p65-p52 dimer in hippocampal nucleus which is reported to have protective effect on hippocampal neurons indicated by unchanged neuronal count in hippocampus in treated groups with respect to control. PMID: 29481885
- study reports an important role for TNFR2 on low-affinity-primed secondary effector CD8+ T cells; results demonstrate the importance of TNF signaling in low-affinity, cross-reactive CD8+ T cell responses during heterologous immunity PMID: 27481849
- our findings implicate TNFR2 in supporting myeloid-derived suppressor cells -mediated immune suppression and metastasis in the liver PMID: 26483205
- TNFR2 blockade appears to disrupt commensal bacteria-host immune symbiosis to reveal autoimmune demyelination in genetically susceptible mice. PMID: 26475926
- TNFR2 protects mice from colitis by inhibiting the expansion of colonic CD8(+) T cells. PMID: 26072395
- To determine the role of TNF-TNFR2/p75 signaling in ischemia-induced inflammation and muscle regeneration, we subjected wild-type and TNFR2/p75 knockouts to hind limb ischemia. PMID: 25466901
- Results demonstrate novel roles of TNFRII in the regulation of Abeta production, suggesting a potential therapeutic strategy for Alzheimer's disease by up-regulating TNFRII levels and elevating phosphorylated IkappaBalpha by SUMOylation. PMID: 24824215
- PGRN protection of endoplasmic reticulum stress induced apoptosis was abolished when TNFR2 signaling was blocked. PMID: 24703938
- TNFR2 signaling by itself is both sufficient and required to induce Vbeta5+ Treg expansion. PMID: 25098294
- TNF-alpha produced by alveolar macrophages induces Th2 cell response, via TNFR2 on Natural killer T cells. PMID: 24191851
- both young and aged mice deficient of TNFR2 performed poorly in the contextual fear conditioning test and displayed decreased anxiety-like behavior PMID: 24135018
- Mice lacking TNFRp75 exhibit greater control of M. tuberculosis infection compared with WT mice. TNFRp75-/- mice developed effective bactericidal granulomas and demonstrated increased pulmonary recruitment of activated dendritic cells. PMID: 24569452
- TNFR2 deficiency significantly impaired transmembrane TNF-alpha-induced release of IL-10 and NO and inhibition of T cell proliferation by MDSC supernatant. TNF-alpha acts as a potent activator of MDSCs via TNFR2 and promotes tumor immune escape. PMID: 24379122
- Our results indicate that TNFR2 signaling is required for the innate airway hyperresponsiveness that develops in obese mice, and suggest that TNFR2 may act by promoting IL-17A, endothelin, and/or trkB expression. PMID: 22584291
- In immune-mediated inflammatory bowel disease models, TNFR2 signaling increases long MLCK expression, resulting in tight junction dysregulation, barrier loss, and induction of colitis. PMID: 23619146
- High Soluble TNFR2 is associated with infarct size and ventricular dysfunction in ST-elevation myocardial infarction PMID: 23405158
- We propose a novel mechanism in which TNF activates TNFR2 on Treg cells and thereby expands this immunosuppressive immune cell population. PMID: 23385062
- In a chronic model of proliferative nephritis, IRF1 and renal-expressed TNFR2 were essential for sustained macrophage accumulation. PMID: 23623383
- Lipopolysaccharide-induced pulmonary alveolar inflammation upregulates alveolar macrophage-mediated production of sTNFR p75. Mechanical ventilator-induced lung injury enhances alveolar epithelial permeability leading to increased sTNFRp75. PMID: 21512145
- results suggest that TNFR2 expressed on podocytes and its canonical NF-kappaB signaling may directly interpose the compound pathogenic responses by podocytes to TNF-alpha, in the absence of other TNFR2-positive renal cell types in proliferative podocytopathies PMID: 21221075
- p75 is essential for tumor angiogenesis and survival in highly vascularized murine lung tumor xenografts. PMID: 22158049
- Both internalization and ASK1-interacting protein-1 association are required for TNFR2-dependent JNK and apoptotic signaling in endothelial cells. PMID: 22743059
- this suggests that the protective effect seen in mice lacking TNFR2 results rather from environment-induced attenuation by low dose bacterial endotoxins than from missing pro-inflammatory signalling via the TNFR2. PMID: 22449555
- TNF-alpha signaling through TNFR2 is essentially involved in the bile acid synthesis and excretion of lipids, resulting in its beneficial effects. PMID: 21850461
- co-activation of TNFR2 during beta-AR stress is anti-inflammatory and counterbalances pro-inflammatory, pro-hypertrophic effects of unopposed TNFR1 activation PMID: 21691899
- expression on non-haematopoietic cells is required for Foxp3+ Treg-cell function and disease suppression in EAE PMID: 22105853
- TNFR1, not TNFR2, mediates TNF-alpha stimulation of dendritic cell maturation and CD8 T cell response to recombinant mouse hepatitis virus infection in vivo. PMID: 21709152
- requirement for TNFR2-expression on murine natural Tregs (nTregs) and induced Tregs (iTregs) in mediating suppression of colitis PMID: 21572024
- These results provide the first in vivo evidence for the origin and regulation of soluble TNFR2 in serum. PMID: 21258364
- TR2 is an important mediator in colitis, and may serve as a therapeutic target in inflammatory bowel disease. PMID: 21347711
- Tumor necrosis factor-alpha signaling via TNFR1/p55 is deleterious whereas TNFR2/p75 signaling is protective in adult infarct myocardium PMID: 21153348
- Mesenchymal stem cell TNFR2 plays an important role in MSC-mediated cardiac protection after myocardial ischemia. PMID: 20160664
- critical for developing macrophages that control of T-cell proliferation PMID: 20518824
- These data indicate that, through TNFR2, microglia may contribute to the counter-regulatory response activated in neuropathological conditions. PMID: 20600925
- These data suggest a protective role for TNFR2/Fas in the pathogenesis of traumatic brain injury. PMID: 20205514
- Intratumoral Tregs expressing more TNFR2 are able to overcome the greater resistance to suppression of intratumoral conventional T cells, resulting in a dominant immunosuppressive tumor environment. PMID: 20525892
- Mouse aorta smooth muscle cells differentiate into lymphoid tissue organizer-like cells on combined tumor necrosis factor receptor-1/lymphotoxin beta-receptor NF-kappaB signaling. PMID: 20139367
- TNFR2(KO) myocardium showed little Etk activation and cell cycle entry but increased ASK1 activation and apoptosis. PMID: 19788501
- TNF type 2 receptor (p75) lowers the threshold of T cell activation PMID: 11739497
- Interaction of TNFR2 with TNF is important in the pathogenesis of wasting associated with acute graft-versus-host-disease (GVHD)and in the generation of histopathological lesions of intestinal GVHD. PMID: 11884480
- CD120b signaling is required for optimal CD40-induced IgM secretion by B cells. PMID: 11907088
- Review. Blockade of the p75TNFR may be helpful in treating systemic autoimmunity since its function may be essential for systemic tissue damage. PMID: 12220546
- Requirement for tumor necrosis factor receptor 2 expression on vascular cells to induce experimental cerebral malaria. PMID: 12228317
- p75 TNF-alpha receptor 2 is required for the regulation of airway hyperresponsiveness (AHR) by gamma delta T cells, since p75-deficient mice develop AHR to a similar degree as control mice following sensitization and challenge with allergen. PMID: 12370348
- TNFRs within the CNS play a significant role in shaping the outcome of TNF signaling during neuroimmune interactions. PMID: 12522266
- Role differs fom that of p55TNFR in the induction of PAI-1. PMID: 12598326
- LPS triggers a rapid shedding of CD120b via a protein kinase C- and Src-independent pathway mediated by p38 MAPK, furin, and metalloproteinase. PMID: 12663667
- TNF/TNFR2 interactions facilitate generation of FasL-dependent CTL effector pathways that play an important role in in vivo antiviral-immune responses in the liver. PMID: 12960267
- Skin tumour multiplicity was significantly reduced in TNFR1-/- and TNFR2-/- mice compared to wild-type mice. PMID: 14661063
- Impaired induction of major histocompatibility complex class I glycoproteins in TNF-R2-deficient mice following axotomy of facial motor nucleus underscores the central role of TNF in the immune activation of the injured nervous system. PMID: 14961564
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亞細胞定位:Membrane; Single-pass type I membrane protein.
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