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  4. Mouse platelet activating factor,PAF ELISA KIT

Mouse platelet activating factor,PAF ELISA KIT

  • 中文名稱(chēng)：
    小鼠血小板活化因子(PAF)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E08199m
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3800/￥2500
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：

    This Mouse PAF ELISA Kit was designed for the quantitative measurement of Mouse PAF protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL .

  • 別名：
    N/A
  • 縮寫(xiě)：
    PAF
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類(lèi)型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測(cè)范圍：
    0.78 ng/mL-50 ng/mL
  • 靈敏度：
    0.195 ng/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Biochemicals
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %91
    Range %86-98
    1:2Average %97
    Range %92-107
    1:4Average %93
    Range %87-98
    1:8Average %87
    Range %82-94
  • 回收率：
    The recovery of mouse PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9287-97
    EDTA plasma (n=4)9795-101
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.576 2.652 2.614 2.460
    252.044 2.076 2.060 1.906
    12.51.443 1.504 1.474 1.320
    6.250.696 0.750 0.723 0.569
    3.120.466 0.504 0.485 0.331
    1.560.354 0.369 0.362 0.208
    0.780.241 0.259 0.250 0.096
    00.153 0.155 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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