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Rat platelet activating factor,PAF ELISA KIT

  • 中文名稱:
    大鼠血小板活化因子(PAF)酶聯(lián)免疫試劑盒
  • 貨號:
    CSB-E07930r
  • 規(guī)格:
    96T/48T
  • 價格:
    ¥3900/¥2500
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:

    This Rat PAF ELISA Kit was designed for the quantitative measurement of Rat PAF protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .

  • 別名:
    N/A
  • 縮寫:
    PAF
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    1.56 ng/mL-100 ng/mL
  • 靈敏度:
    0.39 ng/mL
  • 反應(yīng)時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領(lǐng)域:
    Biochemicals
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PAF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %98
    Range %92-104
    1:2Average %92
    Range %87-98
    1:4Average %89
    Range %86-98
    1:8Average %104
    Range %96-108
  • 回收率:
    The recovery of rat PAF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-92
    EDTA plasma (n=4)9288-96
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.611 2.710 2.661 2.544
    502.058 2.157 2.108 1.991
    251.487 1.506 1.497 1.380
    12.50.899 0.908 0.904 0.787
    6.250.466 0.457 0.462 0.345
    3.120.290 0.281 0.286 0.169
    1.560.182 0.187 0.185 0.068
    00.118 0.115 0.117  
  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

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