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  4. Recombinant Dictyostelium discoideum NEDD8-activating enzyme E1 catalytic subunit (uba3)

Recombinant Dictyostelium discoideum NEDD8-activating enzyme E1 catalytic subunit (uba3)

  • 中文名稱：
    盤基網(wǎng)柄菌uba3重組蛋白
  • 貨號：
    CSB-YP709798DKK
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    盤基網(wǎng)柄菌uba3重組蛋白
  • 貨號：
    CSB-EP709798DKK
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    盤基網(wǎng)柄菌uba3重組蛋白
  • 貨號：
    CSB-EP709798DKK-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    盤基網(wǎng)柄菌uba3重組蛋白
  • 貨號：
    CSB-BP709798DKK
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    盤基網(wǎng)柄菌uba3重組蛋白
  • 貨號：
    CSB-MP709798DKK
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    uba3; ube1c; DDB_G0283891; NEDD8-activating enzyme E1 catalytic subunit; EC 6.2.1.-; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3
  • 種屬：
    Dictyostelium discoideum (Slime mold)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-442
  • 氨基酸序列
    METMDTSVDL PGRWIDIEKI IKRTGPFASP SFEPDTKASP NIMNGLQNDF KVLVIGAGGL GCEILKNLAL SGFRNIDVID MDTIDISNLN RQFLFRRKDV GKSKAEVAAA FINSRITGCN VTPHKCRIQD KDEDYYRQFK IVIAGLDSIE ARRWINGLLV NLVVVNDSGD IEPDTIIPLV DGGTEGFKGQ ARVILPKISS CFECSLDAFP PQVSYAICTI ANTPRVPEHC IQWALLFGLQ DATLEKPFDP KQFDNDNPDH MNWLFECAKK RAEKFNINGV TYKLTQGVAK NIIPAIASTN AIIAAACCNE VFKFCTDSSG YLNNYMMYNG LNGVYTFTFE YEIKEGCAVC GTNLVTFEID KSNTLSTFLE KITTDSRFQF KKPSLRSNGR NLYMQGLLHQ STVPNLEKTL SELNVQEDDE ITITDPALPG NLAVRMRIKY TS
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Regulatory subunit of the dimeric uba3-nae1 E1 enzyme. E1 activates nedd8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a nedd8-uba3 thioester and free AMP. E1 finally transfers nedd8 to the catalytic cysteine of ube2m.
  • 蛋白家族：
    Ubiquitin-activating E1 family, UBA3 subfamily
  • 數(shù)據(jù)庫鏈接：