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  4. Recombinant Drosophila melanogaster Developmental protein eyes absent (eya), partial

Recombinant Drosophila melanogaster Developmental protein eyes absent (eya), partial

  • 中文名稱：
    黑腹果蠅eya重組蛋白
  • 貨號：
    CSB-YP313142DLU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    黑腹果蠅eya重組蛋白
  • 貨號：
    CSB-EP313142DLU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    黑腹果蠅eya重組蛋白
  • 貨號：
    CSB-EP313142DLU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    黑腹果蠅eya重組蛋白
  • 貨號：
    CSB-BP313142DLU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    黑腹果蠅eya重組蛋白
  • 貨號：
    CSB-MP313142DLU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    eya
  • Uniprot No.：
  • 別名：
    eya; cli; CG9554Developmental protein eyes absent; EC 3.1.3.48; Protein Clift
  • 種屬：
    Drosophila melanogaster (Fruit fly)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Tyrosine phosphatase thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. The phosphatase activity was shown in vitro. Appears to function together with So and Dac in eye development. Required for the survival of eye progenitor cells at a critical stage in morphogenesis.
  • 基因功能參考文獻：
    1. This study showed that the eya2 mutants with disrupted vision showed normal mating effects on sleep. PMID: 28851647
    2. speculate that Eya's tyrosine phosphatase activity, although non-essential, confers robustness to RDGN output PMID: 29108015
    3. Uncover a novel role for Eya and So in promoting cell cycle exit in the retinal second mitotic wave (SMW), a synchronized, terminal cell division that occurs several hours after passage of the furrow. We show that Combgap (Cg), a zinc-finger transcription factor, antagonizes Eya-So function in the SMW. PMID: 28619818
    4. By removing gene function in differentiating cells, the study shows that eya is dispensable for larval photoreceptor differentiation, but is required for differentiation during pupal development. PMID: 27257739
    5. our data provide new insight into the cytoplasmic phosphotyrosine signaling networks that operate during photoreceptor axon guidance and suggest specific points of interaction with Eya. PMID: 27194748
    6. In this report we explore the mechanisms by which eya expression is spatially and temporally governed in the developing eye. We demonstrate that multiple cis-regulatory elements function cooperatively to control eya transcription and that spacing between a pair of enhancer elements is important for maintaining correct gene expression PMID: 27930646
    7. A primary function of Eya during Drosophila eye development is as a transcriptional coactivator. PMID: 26980695
    8. Data show that eyes absent protein (Eya) is required in late progenitors to promote eye formation, and that failure to induce the target gene atonal and consequent lack of neuron formation. PMID: 25980363
    9. Drosophila eya is required for normal cone and pigment cell development. PMID: 25057928
    10. Cas and Eya exhibit complementary expression patterns in egg chambers; Cas is restricted to polar and stalk cells from which Eya is excluded. Eya inhibits Cas expression; suppression of Eya is necessary for polar and stalk cell specification. PMID: 23610413
    11. in contrast to ectopic eye formation, MAPK-mediated phosphorylation of Eya on S402 and S407 does not play a role in normal development. PMID: 23251383
    12. data provide the first in vivo demonstration for the immune function of EYA and point to their conserved immune function in response to endogenous DNA, throughout evolution PMID: 22916150
    13. Confocal images of Drosophila third instar eye-antennal discs indicate a subpopulation of retinal progenitor and precursor cells survives within the eya2 mutant eye field. PMID: 23222441
    14. the EGFR pathway is known to regulate the activity of Eya through phosphorylation via MAPK; findings suggest that this pathway is also used to influence eya transcriptional levels PMID: 19884307
    15. Data show that the gene eyes absent (eya) is a key repressor of polar cell fate. PMID: 12403709
    16. Data show that the retinal determination gene eyes absent (eya) is the crucial link between the Hedgehog signaling pathway and photoreceptor differentiation. PMID: 12756186
    17. This protein has a somatic role in spermatocyte development. PMID: 12781687
    18. Our data suggest that EYA requires homo- and heterotypic interactions and RAS/MAPK signaling responsiveness to ensure context-appropriate RD gene network activity. PMID: 12917324
    19. Eyes absent is the prototype for a class of protein tyrosine phosphatases that use a nucleophilic aspartic acid in a metal-dependent reaction; the phosphatase activity of Eyes absent contributes to its ability to induce eye formation in Drosophila PMID: 14628052
    20. Experiments performed in cultured Drosophila cells and in vitro indicate that Eyes absent has intrinsic protein tyrosine phosphatase activity and can autocatalytically dephosphorylate itself PMID: 14628053
    21. dachshund and eyes absent genes have roles in BMP signaling, axon pathfinding and neuronal specification PMID: 15525669
    22. EYA and SO may influence cell proliferation through transcriptional regulation of stg. PMID: 17714699
    23. in addition to its role as a transcription factor, Eya functions as a cytoplasmic protein tyrosine phosphatase PMID: 19217428
    24. analysis of Tinman function reveals Eya and JAK-STAT signaling as essential regulators of muscle development PMID: 19217429

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    HAD-like hydrolase superfamily, EYA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: dme:Dmel_CG9554

    STRING: 7227.FBpp0078964

    UniGene: Dm.5293