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Recombinant Enterobacteria phage T4 Recombination and repair protein (UVSX)

In Stock
  • 中文名稱：
    Recombinant Enterobacteria phage T4 Recombination and repair protein(UVSX)
  • 貨號：
    CSB-EP361289EDZ
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361289EDZ could indicate that this peptide derived from E.coli-expressed Enterobacteria phage T4 (Bacteriophage T4) UVSX.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361289EDZ could indicate that this peptide derived from E.coli-expressed Enterobacteria phage T4 (Bacteriophage T4) UVSX.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    UVSX
  • Uniprot No.：
  • 別名：
    UVSX,Recombination and repair protein
  • 種屬：
    Enterobacteria phage T4 (Bacteriophage T4)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    60.0kDa
  • 表達(dá)區(qū)域：
    1-391aa
  • 氨基酸序列
    MSDLKSRLIKASTSKLTAELTASKFFNEKDVVRTKIPMMNIALSGEITGGMQSGLLILAGPSKSFKSNFGLTMVSSYMRQYPDAVCLFYDSEFGITPAYLRSMGVDPERVIHTPVQSLEQLRIDMVNQLDAIERGEKVVVFIDSLGNLASKKETEDALNEKVVSDMTRAKTMKSLFRIVTPYFSTKNIPCIAINHTYETQEMFSKTVMGGGTGPMYSADTVFIIGKRQIKDGSDLQGYQFVLNVEKSRTVKEKSKFFIDVKFDGGIDPYSGLLDMALELGFVVKPKNGWYAREFLDEETGEMIREEKSWRAKDTNCTTFWGPLFKHQPFRDAIKRAYQLGAIDSNEIVEAEVDELINSKVEKFKSPESKSKSAADLETDLEQLSDMEEFNE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The full-length cDNA ORF (1-391aa) of Enterobacteria phage T4 (Bacteriophage T4) Recombination and repair protein (UVSX) was N-terminally tagged a 6xHis-SUMO and then expressed in E.coli. The Recombinant Enterobacteria phage T4 UVSX protein thus obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, the UVSX protein migrated to an approximately 61 kDa molecular mass band on the SDS-PAGE gel. This protein was also validated by the LC-MS/MS Analysis. UVSX proteins in-stock are available now. This recombinant UVSX protein may be the immunogen for specific antibody production or in the studies of homologous recombination (HR).

    UVSX is the recombinase of the HR machinery. HR plays an important role in repairing the error-free of DNA double-strand breaks and restarting the stagnant replication fork. UVSX is important for the late replication stage of the T4 phage, during which replication initiates by recombination. Together with other recombinases, UVSX forms presynaptic filaments on ssDNA (single-stranded DNA) that are activated to search for homology in dsDNA (double-stranded DNA) and to exchange DNA strand.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
  • 基因功能參考文獻(xiàn)：
    1. the uvsX mutant executes recombination-dependent replication but does it by a qualitatively different way. PMID: 22365497
    2. The authors show that the phage helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a simple nicked circular product rather than complex networks of partially exchanged substrates. PMID: 21035462
    3. UvsX suffice to rescue stalled T4 replication forks in vitro PMID: 15194689
    4. allosteric switch residue His195 controls the formation of an asymmetric conformation of UvsX-ssDNA filaments that is active in DNA strand exchange. PMID: 19027026
    5. unlike wild-type UvsX, the ssDNA-dependent ATPase activities of UvsX-H195Q/A are strongly inhibited by both low and high concentrations of UvsY protein. PMID: 19244311

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  • 蛋白家族：
    RecA family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:1258704