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  4. Recombinant Escherichia coli Melibiose carrier protein (melB), partial

Recombinant Escherichia coli Melibiose carrier protein (melB), partial

  • 中文名稱：
    大腸桿菌melB重組蛋白
  • 貨號(hào)：
    CSB-YP360913ENV
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌melB重組蛋白
  • 貨號(hào)：
    CSB-EP360913ENV
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌melB重組蛋白
  • 貨號(hào)：
    CSB-EP360913ENV-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌melB重組蛋白
  • 貨號(hào)：
    CSB-BP360913ENV
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌melB重組蛋白
  • 貨號(hào)：
    CSB-MP360913ENV
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    melB
  • Uniprot No.：
  • 別名：
    melB; mel-4; b4120; JW4081; Melibiose carrier protein; Melibiose permease; Melibiose transporter; Na+; Li+)/melibiose symporter; Thiomethylgalactoside permease II
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長(zhǎng)度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Responsible for melibiose and other galactoside transport. It is capable of using hydrogen, sodium, and lithium cations as coupling cations for cotransport, depending on the particular sugar transported (symport system).
  • 基因功能參考文獻(xiàn)：
    1. Lys-377 and Ile-22 of melB are crucial for the substrates binding. PMID: 25971963
    2. Data show that the regulatory protein IIA(Glc) directly binds to melibiose permease (MelB) with dissociation constant value 25 muM. PMID: 25296751
    3. Data describe the three-dimensional structure of melibiose permease at 10 angstrom resolution in the membrane plane with cryo-electron microscopy from two-dimensional crystals. PMID: 16139519
    4. Data suggest that loop 4-5 of melibiose permease contributes to coordinated interactions between ion and sugar binding sites, and participates in an electrogenic conformational transition essential for coupled substrate translocation. PMID: 16822867
    5. MelB-N-ethylmaleimide difference spectra are proportionally less disrupted than the R141C ones PMID: 19527646

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  • 亞細(xì)胞定位：
    Cell inner membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Sodium:galactoside symporter (TC 2.A.2) family
  • 數(shù)據(jù)庫(kù)鏈接：