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  4. Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase H (rlmH)

Recombinant Escherichia coli Ribosomal RNA large subunit methyltransferase H (rlmH)

  • 中文名稱：
    大腸桿菌rlmH重組蛋白
  • 貨號：
    CSB-YP364174ENV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    大腸桿菌rlmH重組蛋白
  • 貨號：
    CSB-EP364174ENV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    大腸桿菌rlmH重組蛋白
  • 貨號：
    CSB-EP364174ENV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    大腸桿菌rlmH重組蛋白
  • 貨號：
    CSB-BP364174ENV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    大腸桿菌rlmH重組蛋白
  • 貨號：
    CSB-MP364174ENV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    rlmH
  • Uniprot No.：
  • 別名：
    rlmH; ybeA; b0636; JW0631; Ribosomal RNA large subunit methyltransferase H; EC 2.1.1.177; 23S rRNA; pseudouridine1915-N3)-methyltransferase; 23S rRNA m3Psi1915 methyltransferase; rRNA; pseudouridine-N3-)-methyltransferase RlmH
  • 種屬：
    Escherichia coli (strain K12)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-155
  • 氨基酸序列
    MKLQLVAVGT KMPDWVQTGF TEYLRRFPKD MPFELIEIPA GKRGKNADIK RILDKEGEQM LAAAGKNRIV TLDIPGKPWD TPQLAAELER WKLDGRDVSL LIGGPEGLSP ACKAAAEQSW SLSALTLPHP LVRVLVAESL YRAWSITTNH PYHRE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA. Specific for fully assembled 70S ribosomes.
  • 基因功能參考文獻：
    1. Data suggest that E. coli RlmH functions as a dimer, with the S-adenosyl-methionine binding pocket of one monomer and the C-terminal residues of its counterpart forming a composite active site. Study proposes that the C-terminal tail provides a residue that acts as a general base while the other conserved residues form a network of interactions that organize the catalytic site. PMID: 28428565
    2. While the synthesis of Psis in H69 by RluD is relatively insensitive to the point mutations at neighboring positions, methylation of one of the Psis by RlmH exhibited a much stronger sensitivity. PMID: 22586702
    3. show that RlmH has unique substrate specificity among rRNA modification enzymes PMID: 20817755
    4. The identity of the methyltransferase YbeA (redesignated RlmH for rRNA large subunit methyltransferase H) that specifically catalyzes methyl group addition to form m(3)Psi1915 is reported. PMID: 18755835
    5. E. coli protein YbeA as the methyltransferase methylating Psi1915 in 23S rRNA was identified. PMID: 18755836

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    RNA methyltransferase RlmH family
  • 數(shù)據(jù)庫鏈接：