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  4. Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter (vacA), partial

Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter (vacA), partial

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  • 中文名稱：
    幽門螺旋桿菌vacA重組蛋白
  • 貨號：
    CSB-EP345927HUV
  • 規(guī)格：
    ￥2328
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP345927HUV could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP345927HUV could indicate that this peptide derived from E.coli-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) vacA.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    vacA
  • Uniprot No.：
  • 別名：
    vacA; HP_0887; Vacuolating cytotoxin autotransporter [Cleaved into: Vacuolating cytotoxin; Vacuolating cytotoxin translocator]
  • 種屬：
    Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
  • 蛋白長度：
    Partial
  • 來源：
    E.coli
  • 分子量：
    43.6kDa
  • 表達(dá)區(qū)域：
    1018-1290aa
  • 氨基酸序列
    KYEKPTNVWANAIGGTSLNNGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFNNQANSLNSGANNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSNSNQVALKNGSSSQHLFNASANVEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNAAHNPLSTHARVMMGGELKLAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-B2M-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter(vacA) is a partial-length protein derived from E.coli. The expression region of this protein is the 1018-1290aa of Helicobacter pylori vacA. It carries an N-terminal 6xHis-B2M-tag and has a calculated molecular weight of 43.6 kDa. Its purity is greater than 90% as determined by SDS-PAGE and its identity was validated by the LC-MS/MS Analysis. This recombinant vacA protein may be used to generate specific antibodies and in microbiology research.

    VacA is one of the predominant protein toxins secreted by the type V auto-transport secretion system of the Helicobacter pylori (H. pylori). The H. pylori is a pathogenic bacterium that potentially causes peptic ulcers of the human stomach even gastric cancer. VacA is important for H. pylori colonization in vivo and has the capacity of causing "vacuole"-like membrane vesicles in the cytoplasm of gastric cells. Besides, it has other effects on target cells, including disturbing mitochondrial functions, stimulating apoptosis, and blocking T cell proliferation.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions.
  • 基因功能參考文獻(xiàn)：
    1. Helicobacter pylori VacA induces autophagic cell death in gastric epithelial cells via the endoplasmic reticulum stress pathway. PMID: 29238039
    2. The results indicate that cortactin is involved in the regulation of apoptosis induced by VacA in gastric cells. PMID: 26289258
    3. Genotype testing of vacA s- and m- region will be useful in screening susceptible individuals for duodenal ulcer development. PMID: 25063579
    4. These results suggested that cagA and vacA gene expression is upregulated in Helicobacter pylori, especially by host cell contact, and Fur has a role in the upregulation. PMID: 24020886
  • 亞細(xì)胞定位：
    [Vacuolating cytotoxin autotransporter]: Periplasm.; [Vacuolating cytotoxin]: Secreted. Cell surface.; [Vacuolating cytotoxin translocator]: Cell outer membrane; Multi-pass membrane protein.
  • 數(shù)據(jù)庫鏈接：

    KEGG: hpy:HP0887

    STRING: 85962.HP0887