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  4. Recombinant Human DNA polymerase zeta catalytic subunit (REV3L), partial

Recombinant Human DNA polymerase zeta catalytic subunit (REV3L), partial

  • 中文名稱：
    人REV3L重組蛋白
  • 貨號：
    CSB-YP019577HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人REV3L重組蛋白
  • 貨號：
    CSB-EP019577HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人REV3L重組蛋白
  • 貨號：
    CSB-EP019577HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人REV3L重組蛋白
  • 貨號：
    CSB-BP019577HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人REV3L重組蛋白
  • 貨號：
    CSB-MP019577HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    REV3L
  • Uniprot No.：
  • 別名：
    REV3L; POLZ; REV3; DNA polymerase zeta catalytic subunit; EC 2.7.7.7; Protein reversionless 3-like; REV3-like; hREV3
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translesion DNA synthesis (TLS). Lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function.
  • 基因功能參考文獻：
    1. Following challenge with AFB1, survival of mouse cells deficient in pol zeta (Rev3L(-/-)) was significantly reduced relative to Rev3L(+/-) cells or Rev3L(-/-) cells complemented through expression of the wild-type human REV3L PMID: 27849610
    2. These results demonstrate a previously unrecognized relationship between p53 and REV3L in cancer cell metabolism and may lead to improvements in chemotherapy treatment plans that reduce cisplatin resistance in lung cancer. PMID: 29307819
    3. REV3/ATR knockdown enhances the cytotoxicity of cisplatin in non-small cell lung cells. PMID: 28075014
    4. The data directly show that, in the human genome, DNA Pol-eta and Rev1 bypass cyclobutane pyrimidine dimers and 6-4PP at replication forks, while only 6-4PP are also tolerated by a Rev3L-dependent gap-filling mechanism, independent of S phase. PMID: 27095204
    5. The results indicate that human DNA polymerase zeta plays important roles in induction of mutations, clastogenicity and in cellular survival of the damaged human cells. PMID: 27338670
    6. loss-of-function of REV3L dramatically enhanced the sensitivity of SCCHN cells to dacomitinib by the loss of both translesion synthesis and homologous recombination pathways. PMID: 26790612
    7. REV3L plays an important role in esophageal squamous cell carcinoma (ESCC) progression and chemoresistance, and is a potential diagnostic marker and therapeutic target for ESCC PMID: 26752104
    8. REV3 functions in mammalian mitochondria and that mitochondrial REV3 is associated with the tumorigenic potential of cells. PMID: 26462070
    9. Taken together, we demonstrated that inhibition of REV3L sensitized lung cancer H1299 cells to cisplatin treatment PMID: 26165320
    10. finding that PLXND1 and REV3L mutations are responsible for a proportion of MBS patients suggests that de novo mutations in other genes might account for other MBS patients PMID: 26068067
    11. data suggest that REV3L plays an important role in regulating cervical cancer cellular response to cisplatin PMID: 25781640
    12. Rev3, the catalytic subunit of polymerase zeta (Pol zeta), is involved in DNA replication under conditions of replication stress caused by deoxyribonucleotide shortage and/or imbalance in human lung adenocarcinoma cells. PMID: 25113059
    13. Single nucleotide polymorphisms in RBPJ, IL1R1, REV3L, TRAF3IP2, IRF1 and ICOS showed association with rheumatoid arthritis in black South Africans. PMID: 25014791
    14. Our results suggest for the first time that REV1 and REV3L SNPs might serve as potential predictive markers of outcome of cisplatin-based chemotherapy PMID: 24956248
    15. Human Pol eta inserts a nucleotide opposite the lesion, followed by Pol zeta extending the DNA primer; thus, the two complement each other to fully bypass the cisplatin cross-link. PMID: 24449906
    16. DNA polymerase zeta is a predictor of poor prognosis for cervical cancer patients who are resistant to chemoradiation. PMID: 23456618
    17. A long-term depletion of Rev3 in cultured human cells results in massive genomic instability and severe cell cycle arrest. PMID: 23303771
    18. REV3L rs465646 variant modifies lung cancer susceptibility in Chinese Han population by affecting miRNA-mediated gene regulation PMID: 22349819
    19. analysis of the crystal structure of the ternary complex composed of the C-terminal domain of human REV1, REV7, and a REV3 fragment PMID: 22859296
    20. ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment has been crystallized. The crystals belonged to space group P3(1)21, with unit-cell parameters a = b = 74.7, c = 124.5 A PMID: 22869133
    21. our data suggest a significant role of genetic variation in the polymerase zeta subunit genes regarding the development and progression of BC. PMID: 21455670
    22. DNA polymerase zeta transgene participates directly in immunoglobulin hypermutation. PMID: 22547703
    23. REV7 subunit of pol zeta mediated the interaction between REV3 and the REV1 C terminus. PMID: 22303021
    24. The REV1/Polzeta complex maintains genomic stability by directly participating in DNA double-stranded break repair. PMID: 21926160
    25. Findings indicate that depletion of REV3 not only can amend cisplatin-based cancer therapy but also can be applied for susceptible cancers as a potential monotherapy. PMID: 22028621
    26. Data suggest that REV3 plays an important role in different cellular growth periods and physiological conditions. PMID: 20466635
    27. function of REV7 as an adapter protein to recruit Polzeta to a DNA lesion site PMID: 20164194
    28. show the first crystal structure of REV7 in complex with a fragment of REV3 polymerase (residues 1847-1898) and reveal the mechanism underlying REV7-REV3 interaction PMID: 20164194
    29. REV1 and Polzeta facilitate repair of interstrand cross-links independently of PCNA monoubiquitination and Poleta, whereas RAD18 plus Poleta, REV1, and Polzeta are all necessary for replicative bypass of cisplatin intrastrand DNA cross-links. PMID: 20028736
    30. To clarify the structural basis of the interaction between REV7 and REV3, REV7 was crystallized in complex with a REV3 fragment. PMID: 20054135
    31. REV3L Overexpression confers chemoresistance to cisplatin in gliomas. PMID: 19289490
    32. plays a critical role in the induction of mutations PMID: 12459444
    33. REV3 gene plays a role not only in lesion-targeted DNA mutagenesis, but also in mutagenesis on undamaged DNA templates that is called untargeted mutation. PMID: 12717825
    34. downregulation of rev3 might have occurred early during tumorigenesis. PMID: 18622427
    35. The study revealed that suppression of REV3 delayed spontaneous S phase progression and suppression of REV3 limits spontaneous and DNA damage induced mutagenesis. PMID: 18779149
    36. DNA polymerase zeta cooperates with polymerases kappa and iota in translesion DNA synthesis across pyrimidine photodimers in cells from xeroderma pigmentosum variant patients. PMID: 19564618

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-B family
  • 組織特異性：
    Ubiquitously expressed.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9968

    OMIM: 602776

    KEGG: hsa:5980

    STRING: 9606.ENSP00000351697

    UniGene: Hs.232021