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  4. Recombinant Human Kinesin-like protein KIF14 (KIF14), partial

Recombinant Human Kinesin-like protein KIF14 (KIF14), partial

  • 中文名稱：
    人KIF14重組蛋白
  • 貨號：
    CSB-YP613582HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人KIF14重組蛋白
  • 貨號：
    CSB-EP613582HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人KIF14重組蛋白
  • 貨號：
    CSB-EP613582HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人KIF14重組蛋白
  • 貨號：
    CSB-BP613582HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人KIF14重組蛋白
  • 貨號：
    CSB-MP613582HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    KIF14
  • Uniprot No.：
  • 別名：
    KIAA0042; KIF 14; KIF14; KIF14_HUMAN; Kinesin Family Member 14; Kinesin like protein KIF14; Kinesin-like protein KIF14; MGC142302
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity. Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis. During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively. Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1. During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division. Also is required for chromosome congression and alignment during mitotic cell cycle process. Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules.
  • 基因功能參考文獻(xiàn)：
    1. KIF14 missense and loss of function variants were identified in intellectual disability with microcephaly patients. PMID: 29343805
    2. KIF14 is upregulated in PCa cell lines and clinical PCa tissues. Inhibition of KIF14 suppresses cell proliferation, induces G2 arrest and apoptosis. PMID: 28525372
    3. In particular, counteracting forces between minus-end-directed kinesin-14 and plus-end-directed kinesin-5 motors have recently been implicated in the regulation of microtubule nucleation. PMID: 28668932
    4. The mechanochemical cycle of kinesin-14 has been reported. PMID: 27729532
    5. Data indicate that three genes, KIF14, NCAPG and CENPE that were upregulated in Pediatric high-grade gliomas (pHGGs) and were direct miR-137 or miR-6500-3p targets. PMID: 26933822
    6. This study identified the mutations in KIF14 as a new cause of primary microcephaly and using cellular experiments demonstrate that impaired cytokinesis, increased apoptosis, and reduced cell motility are features of the associated pathogenesis. PMID: 28892560
    7. Signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14 to properly coordinate cytokinesis. PMID: 28630147
    8. Studied overexpression of KIF14 which showed the tight correlation between KIF14 upregulation and inferior clinical outcome in medulloblastoma (MB), suggesting KIF14 is a potential negative prognostic marker in MB. Transient, siRNAs-mediated downregulation of KIF14 suppressed cell proliferation and induced apoptosis in two MB cell lines. PMID: 28504687
    9. study suggests that KIF14 may serve as a predictor of poor survival and a novel prognostic biomarker of chemoresistance to paclitaxel treatment in cervical cancer. PMID: 27128470
    10. analysis of epigenetic regulation of KIF14 overexpression in ovarian cancer PMID: 24626475
    11. Kinesin-14 blocks microtubule nucleation in yeast and reveal that this inhibition is countered by the kinesin-5 protein, Cut7.[Cut7, Pkl1] PMID: 25348260
    12. High-grade serous ovarian cancer cells may depend on KIF14 for in vitro proliferation. PMID: 25528264
    13. Mutations in KIF14 identified as a novel cause of an autosomal recessive lethal fetal ciliopathy phenotype. PMID: 24128419
    14. critical role for KIF14 in the tumorigenic potential of triple-negative breast cancer PMID: 24784001
    15. High KIF14 expression is associated with hepatocellular carcinoma. PMID: 25106407
    16. KIF14 knockdown downregulates the expression of Skp2 and Cks1, leading to accumulation of p27Kip1. PMID: 24854087
    17. KIF14 inhibits tumor growth and cancer metastasis in lung adenocarcinoma. PMID: 23626713
    18. Data indicate that KIF14 and TLN1 loss-of-function significantly enhanced chemosensitivity in four triple-negative breast cancer (TNBC) cell lines. PMID: 23479679
    19. Suppression of KIF14 not only decreases cancer cell migration but also induces apoptosis of cells. PMID: 23414349
    20. KIF14 expression in gliomas is tumor-specific and increased in more aggressive tumors. KIF14 might function as a candidate prognostic marker for human gliomas. PMID: 22999822
    21. It was shown that the kinesin KIF14 associates with the PDZ domain of Radil and negatively regulates Rap1-mediated inside-out integrin activation by tethering Radil on microtubules. PMID: 23209302
    22. Findings indicate that KIF14 mRNA is an independent prognostic marker in serous ovarian cancer. PMID: 21618518
    23. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20306291
    24. Primary non-small-cell lung carcinoma overexpressing KIF14 showed a trend toward decreased survival. PMID: 15897902
    25. During cytokinesis, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. PMID: 16431929
    26. RNA interference-mediated silencing of KIF14 disrupts cell cycle progression and induces cytokinesis failure. PMID: 16648480
    27. KIF14 is expressed most highly in squamous cell carcinoma, then in large-cell undifferentiated carcinoma, then adenocarcinoma PMID: 17545527
    28. Overexpression of KIF14 was confirmed in primary human retinoblastoma and showed that patients with an older age at diagnosis express significantly higher levels of KIF14. PMID: 17962437
    29. KIF14 overexpression is associated with papillary renal cell tumors with chromosome 1q duplication. PMID: 19123481
    30. report confirms significant mRNA overexpression of KIF14 and E2F3 together in a large cohort of retinoblastoma tumors PMID: 19190782
    31. Results show that the established nerve invasion model and the consensus signature of perineural invasion could be instrumental in the identification of novel therapeutic targets of pancreatic cancer as exemplified by KIF14 and ARHGDIbeta. PMID: 19509238
    32. Four genes previously not examined in that respect in laryngeal carcinoma, occurred to be good markers of the neoplasm. They are: metal-proteinase ADAM12, cyclin-dependent kinase 2-CDK2, kinesin 14-KIF14, suppressor 1 of checkpoint-CHES1. PMID: 19609547
    33. KIF14 mRNA expression is prognostic for disease-free survival in non-small-cell lung cancer. Knockdown inhibits colony formation ability. PMID: 17545527
    34. The KIF14 gene is amplified in retinoblastoma; likely an early genomic change in this cancer. PMID: 17099872
    35. KIF14 is overexpressed in primary breast cancer; predicts disease-free and overall survival PMID: 16570270
    36. Overexpressed in primary retinoblastoma and lung cancer, and in medulloblastoma and breast cancer cell lines. Low expression in normal adult tissue; highly expressed in fetal liver and thymus. PMID: 15897902

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  • 相關(guān)疾病：
    Meckel syndrome 12 (MKS12)
  • 亞細(xì)胞定位：
    Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Midbody.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 19181

    OMIM: 611279

    KEGG: hsa:9928

    STRING: 9606.ENSP00000356319

    UniGene: Hs.3104