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Recombinant Human Lamin-B1 (LMNB1)

  • 中文名稱：
    人LMNB1重組蛋白
  • 貨號：
    CSB-YP013004HU
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    人LMNB1重組蛋白
  • 貨號：
    CSB-EP013004HU
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    人LMNB1重組蛋白
  • 貨號：
    CSB-EP013004HU-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    人LMNB1重組蛋白
  • 貨號：
    CSB-BP013004HU
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    人LMNB1重組蛋白
  • 貨號：
    CSB-MP013004HU
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    2-583
  • 氨基酸序列
    ATATPVPPR MGSRAGGPTT PLSPTRLSRL QEKEELRELN DRLAVYIDKV RSLETENSAL QLQVTEREEV RGRELTGLKA LYETELADAR RALDDTARER AKLQIELGKC KAEHDQLLLN YAKKESDLNG AQIKLREYEA ALNSKDAALA TALGDKKSLE GDLEDLKDQI AQLEASLAAA KKQLADETLL KVDLENRCQS LTEDLEFRKS MYEEEINETR RKHETRLVEV DSGRQIEYEY KLAQALHEMR EQHDAQVRLY KEELEQTYHA KLENARLSSE MNTSTVNSAR EELMESRMRI ESLSSQLSNL QKESRACLER IQELEDLLAK EKDNSRRMLT DKEREMAEIR DQMQQQLNDY EQLLDVKLAL DMEISAYRKL LEGEEERLKL SPSPSSRVTV SRASSSRSVR TTRGKRKRVD VEESEASSSV SISHSASATG NVCIEEIDVD GKFIRLKNTS EQDQPMGGWE MIRKIGDTSV SYKYTSRYVL KAGQTVTIWA ANAGVTASPP TDLIWKNQNS WGTGEDVKVI LKNSQGEEVA QRSTVFKTTI PEEEEEEEEA AGVVVEEELF HQQGTPRASN RSC
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
  • 基因功能參考文獻(xiàn)：
    1. Results show that HECW2 interacts with lamin B1 mediating its ubiquitination and proteasomal degradation. PMID: 29753763
    2. down-regulation of Lamin B1 and up-regulation of Nephroblastoma overexpressed (NOV) are at least partially responsible for the inhibitory effect of Huaier on the proliferative and invasive capacity of SKHEP-1 cells PMID: 27503760
    3. The aim of the present study was to elucidate the influence of LMNB1 upregulation on colon cancer cell line after treatment with 5-FU. The results indicate, that overexpression of LMNB1 induced dose-dependent cell death mainly by mitotic catastrophe pathway. PMID: 29115590
    4. We show that epithelial cells failing to undergo proliferation arrest during TGF-beta-induced EMT sustain mitotic abnormalities due to failed cytokinesis, resulting in aneuploidy. This genomic instability is associated with the suppression of multiple nuclear envelope proteins implicated in mitotic regulation and is phenocopied by modulating the expression of LaminB1. PMID: 27926867
    5. An upstream mutation alters LMNB1 gene expression in autosomal dominant adult-onset demyelinating leukodystrophy. PMID: 25701871
    6. Deregulation of LMNB1 expression induces modified splicing of several genes, likely driven by raver-2 overexpression, and suggest that an alteration of mRNA processing could be a pathogenic mechanism in adult-onset autosomal dominant leukodystrophy. PMID: 25637521
    7. Lamin B1 levels are increased in oligodendrocytes, the cell type that produces myelin in the central nervous system. PMID: 26311780
    8. Nuclear envelope remodelling during human spermiogenesis involves somatic B-type lamins and a spermatid-specific B3 lamin isoform. PMID: 25477337
    9. LMNB1-related autosomal-dominant leukodystrophy is a slowly progressive neurological disease with survival lasting more than two decades after clinical onset. PMID: 26053668
    10. Lamin B1 overexpression increases nuclear rigidity in autosomal dominant leukodystrophy fibroblasts. PMID: 24858279
    11. Data indicate that lamin B1 promotes DNA double-strand breaks (DSBs) repair and cell survival. PMID: 25733566
    12. maintenance of lamin B1 levels is required for DNA replication and repair through regulation of the expression of key factors involved in these essential nuclear functions PMID: 25535332
    13. This led us to propose a model where the nucleolus has steady-state stiffness dependent on ribosome biogenesis activity and requires LaminB1 for its flexibility. PMID: 24297448
    14. The regulation of lamin B1 is important for cellular physiology and disease.To how perturbations of lamin B1 affect cellular physiology and discuss the implications this has on senescence, HGPS and ADLD. PMID: 23873483
    15. LMNB1 is required to maintain chromatin condensation in interphase nuclei. PMID: 24732130
    16. Lamin B1 plays an important role in pancreatic cancer pathogenesis and is a novel therapeutic target of betulinic acid treatment. PMID: 23857605
    17. we confirm the underlying role of lamin B1 duplication, regardless of the autonomic malfunction onset in Adult-onset autosomal dominant leukodystrophy PMID: 23681646
    18. detailed molecular analysis of the largest collection of autosomal dominant leukodystrophy (ADLD) families studied, to date; identified the minimal duplicated region necessary for the disease, defined all the duplication junctions at the nucleotide level and identified the first inverted LMNB1 duplication PMID: 23649844
    19. Rare variants of LMNB1 may contribute to susceptibility to neural tube defects. PMID: 23733478
    20. lamin B1 down-regulation in senescence is a key trigger of global and local chromatin changes that impact gene expression, aging, and cancer PMID: 23934658
    21. LMNB1 may contribute to senescence in at least two ways due to its uneven genome-wide redistribution: first, through the spatial reorganization of chromatin and, second, through gene repression. PMID: 23964094
    22. Treating normal human fibroblasts with farnesyltransferase inhibitors causes the accumulation of unprocessed lamin B2 and lamin A and a decrease in mature lamin B1. PMID: 23475125
    23. Results indicate that lamin B1 (LMNB1) accumulation in adult-onset autosomal dominant leukodystrophy (ADLD) is associated with Oct-1 recruitment. PMID: 23261988
    24. LMNB1 protein levels decline in senescent human dermal fibroblasts and keratinocytes, mediated by reduced transcription and inhibition of LMNB1 messenger ribonucleic acid translation by miRNA-23a. PMID: 23439683
    25. Lamin B1 is lost from primary human and murine cell strains when they are induced to senesce. PMID: 22496421
    26. This study demonistrated that Adult-onset autosomal dominant leukodystrophy due to LMNB1 gene duplication. PMID: 21909802
    27. The authors show that oxidative stress increases lamin B1 levels through p38 Mitogen Activated Protein kinase activation. PMID: 22246186
    28. crystal structures of lamin B1 globular tail domain and coiled 2B domain, with similar folds to Ig-like domain and coiled-coil domain of lamin A. Found an extra intermolecular disulfide bond in lamin B1 coil 2B domain, which does not exist in lamin A/C. PMID: 22265972
    29. LB1 expression in WI-38 cells decreases during cellular senescence PMID: 22155925
    30. SNP array analysis revealed novel duplications spanning the entire LMNB1 gene in probands from each of four adult-onset autosomal dominant leukodystrophy families PMID: 21225301
    31. Autosomal dominant leukodystrophy is the first disease that has ever been linked to lamin B1 mutations and it expands the pathological role of the nuclear lamia to include disorders of the brain. PMID: 20816241
    32. results suggest that a LMNB1 regulatory sequence mutation underlies the variant adult-onset autosomal dominant leukodystrophy (ADLD) phenotype; adult forms of ADLD linked to 5q23 may be more heterogeneous clinically and genetically than previously thought PMID: 19961535
    33. Proteomics identified lamin B1 as being significantly upregulated in HCC tumors and present in patients' plasma. PMID: 19522540
    34. colocalizes with lamin B1 in the nucleoplasm and around the nuclear rim during S-phase of cells transfected with EBNA-1 in the absence of EBV plasmids. PMID: 12898336
    35. organization of the nuclear envelope and lamina is dependent on a mechanism involving the methylation of lamin B1 PMID: 14504265
    36. We now show that epitope masking in the nucleus is often responsible for failure to detect emerin and lamins in human, rat and pig tissues.These data suggest that different regions of the lamin B1 molecule are masked in different tissues. PMID: 16283426
    37. Apoptotic neutrophils express lamin B1 on their surface; these cells may participate in the development of autoantibodies directed against cytoskeletal proteins, a condition frequently reported in several inflammatory diseases. PMID: 16365157
    38. lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors; propose that lamin B is a structural component of the spindle matrix that promotes microtubule assembly and organization in mitosis PMID: 16543417
    39. Results show that a lamin B1-containing nucleoskeleton is required to maintain RNA synthesis and that ongoing synthesis is a fundamental determinant of global nuclear architecture in mammalian cells. PMID: 18334554
    40. Novel duplication on chromosomal band 5q23.2 in a French Canadian family with autosomal dominant leukodystrophy that supports the implication of duplicated LMNB1 as the disease-causing mutation. PMID: 19001169
    41. duplication of the lamin B1 gene (LMNB1) has recently been described in a rare form of autosomal dominant adult-onset leukoencephalopathy. PMID: 19151023
    42. Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner. PMID: 19198602
    43. Our work indicates that lamin B1 defects are probably not responsible for signs and symptoms resembling multiple sclerosis. PMID: 19348623
    44. Lamin B1 maintains the functional plasticity of nucleoli. PMID: 19383719

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  • 相關(guān)疾?。?/div>
    Leukodystrophy, demyelinating, autosomal dominant, adult-onset (ADLD)
  • 亞細(xì)胞定位：
    Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side.
  • 蛋白家族：
    Intermediate filament family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 6637

    OMIM: 150340

    KEGG: hsa:4001

    STRING: 9606.ENSP00000261366

    UniGene: Hs.89497