Recombinant Human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A)
In Stock-
中文名稱:人PPARGC1A重組蛋白
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貨號:CSB-EP871382HUa0
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規(guī)格:¥1836
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圖片:
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其他:
產(chǎn)品詳情
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純度:Greater than 90% as determined by SDS-PAGE.
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基因名:
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Uniprot No.:
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種屬:Homo sapiens (Human)
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蛋白長度:Full Length
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來源:E.coli
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分子量:95.1 kDa
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表達區(qū)域:1-798aa
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氨基酸序列MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. -
蛋白標簽:N-terminal 6xHis-tagged
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產(chǎn)品提供形式:Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
緩沖液:If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
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復溶:We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件:Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質(zhì)期:The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期:3-7 business days
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注意事項:Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet & COA:Please contact us to get it.
相關(guān)產(chǎn)品
靶點詳情
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功能:Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
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基因功能參考文獻:
- LL was significantly negatively correlated with PGC-1alpha, TNF-alpha, and IL-6 mRNA expressions. PGC-1alpha mRNA expression levels in paraspinal muscles may be affected by lumbar kyphosis. PMID: 30233161
- PGC1A can improve mitochondrial biogenesis and negatively regulate senescence, although this function of PGC1A in age-related macular degeneration needs further studies. Post-translational modifications of PGC1A by AMPK (AMP kinase) and SIRT1 (sirtuin 1) are crucial for its activation and important in age-related macular degeneration pathogenesis. PMID: 30087287
- study provides support for the involvement of both NFE2L2 and PPARGC1alpha in Parkinson's disease susceptibility and progression, marginally and through pathways involving maneb and paraquat exposure. PMID: 29630901
- Increased nuclear p53 phosphorylation and PGC-1alpha protein content immediately following SIE but not CE suggests these may represent important early molecular events in the exercise-induced response to exercise PMID: 28281651
- The transcriptional co-activator peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1alpha) is proposed to coordinate skeletal muscle mitochondrial biogenesis through the integrated induction of nuclear- and mitochondrial-encoded gene transcription--{REVIEW} PMID: 29126696
- The changes of epigenetic modification of PGC-1alpha gene in pregnant women with gestational diabetes mellitus may be a mechanism of abnormal glucose metabolism in offspring. PMID: 29730292
- Our results showed that administration of Hcy reduced the SIRT1/AMPK/PGC-1alpha signaling in chondrocytes, leading to mitochondrial dysfunction as a result of increased oxidative stress and apoptosis PMID: 29413962
- Study in melanoma cell and in vivo melanoma models provides evidence for a direct role of BRD4 binding at super-enhancers that drive the expression of PGC-1alphaalpha and SOX10, a transcription factor involved in melanocyte development. PMID: 28991225
- the present study provided evidence that cisplatin stimulated the expression of PGC1A and the upregulation of mitochondrial biogenesis through PGC1A, promoting cell viability and inhibiting apoptosis in response to cisplatin treatment, thus triggering cisplatin resistance in ovarian cancer cells. PMID: 29749474
- At least in the subcutaneous fat of humans and mice, the levels of PGC-1a decrease during obesity, while its physical association with A20 increases. PMID: 29678181
- The non-responders for aerobic training are the carriers of PPARGC1A rs8192678 Ser/Ser. The best responders to aerobic training are PPARGC1A rs8192678 Gly/Gly carriers. PMID: 29762540
- Pathological cardiac hypertrophy, decrease of PGC-1alpha activity, and reactivation of the fetal genetic program in chronic heart failure are demonstrated. PMID: 29658071
- The findings suggest that activation of TGR5 promoted mitochondrial biogenesis in endothelial cells, which is mediated by the CREB/PGC-1a signaling pathway. PMID: 29709472
- that PGC-1alpha may be useful for predicting nodal metastasis, and may represent a biomarker for poor prognosis in colorectal cancer PMID: 29566941
- Koreans with the minor alleles of PGC-1alpha rs10517030, rs10517032 and rs10212638 are at greater risk of type 2 diabetes mellitus. PMID: 28488691
- It was found that down-regulated PGC-1alpha expression is independently associated with the development of CVD in HD patients. PMID: 28550474
- Results provide evidence that PGC-1A regulates FASN expression level in colorectal cancer cells. PMID: 29130104
- PPARGC1A expression is differentially regulated via canonical and alternative promoters in trained and untrained skeletal muscle. PMID: 29233908
- the results reveal that PGC-1alpha stimulates bioenergetic potential, which promotes breast cancer metastasis and facilitates adaptation to metabolic drugs. PMID: 28988825
- No associations were observed between SNPs in PPARGC1 and obesity. PMID: 28947843
- Results show that PGC1 function is modulated by ERRa which physically interacts with PGC1a, promoting the expression of genes involved in mitochondrial oxidative phosphorylation, but does not affect expression of the cellular antioxidant or invasive/metastatic programs. PMID: 28596418
- PGC-1alpha induction during differentiation is required for both mitochondrial biogenesis and commitment to the hepatocytic lineage, and TCF7L2 repression is sufficient to increase PGC-1alpha expression, mitochondrial biogenesis and OXPHOS activity. PMID: 28795454
- AMPK-PGC-1a control of mitochondrial reactive oxygen species regulates Warburg metabolism. PMID: 28978464
- Our results suggest that maternal inflammation programmed proneness to NF-kappaB over-activation which impaired PGC-1a-mediated anti-oxidant capacity resulting in the increased sensitivity of offspring to hypertensive damage. PMID: 27616627
- Postexercise cold water immersion of one limb also enhances PGC-1alpha expression. PMID: 28546467
- Multiple regression analysis showed that age, male gender, body max index, presence of obesity, type-2-diabetes mellitus, hypertension and coronary artery disease and left ventricular ejection fraction were associated with the expression levels of UCP1, PGC1alpha and PRDM16 mRNA PMID: 28824327
- This is the first study regarding the relationship of PPARGC1A and ADIPOQ polymorphisms, and the aggressiveness of prostate cancer in men with overweight or obesity. PMID: 28453464
- The effects of PGC-1 gene Gly482Ser polymorphisms on alterations in glucose and lipid metabolism induced by exercise training. PMID: 27699582
- acute upregulation of PGC-1alpha mRNA relates to the magnitude of subsequent training-induced increases in oxidative capacity, but not other molecular and morphological chronic skeletal muscle adaptations PMID: 28177701
- Data show a significant differential pattern of genetic association of peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PGC-1alpha) among ethnic groups emphasizing the role of ethnicity towards disease susceptibility [Meta-analysis]. PMID: 29063962
- Findings suggest that despite their proposed involvement in the regulation of inflammatory processes in multiple sclerosis (MS), PPARalpha, PPARbeta/delta, and PGC-1alpha proteins are not potential biomarkers of neuroinflammation in MS, and indicate a preferential role of PPARgamma in the endogenous regulation of autoimmune response in the human central nervous system within its receptor family. PMID: 28483651
- this study shows that PPARGC1A rs3736265 G>A polymorphism is associated with decreased risk of type 2 diabetes mellitus and fasting plasma glucose PMID: 28418876
- Suggest that a pro-inflammatory diet may have a differential effect on colorectal cancer risk based on PPARGC1A genetic variation. PMID: 28051997
- that oxidatively-mediated reductions in parkin solubility and function in a mouse model of age-related sporadic PD coincides with increased PARIS levels and reduced PGC-1alpha signaling PMID: 27185595
- Study found that the metabolic master regulator PGC-1alpha is differentially affected by ALS-associated mutations in brain vs. peripheral tissues. Increased PGC-1alpha activity in peripheral tissue contributes to the metabolic phenotype, while in the CNS blunting of the PGC-1alpha response renders motoneurons vulnerable. PMID: 27818323
- A single nucleotide variant of the PPARGC1A gene (rs8192678) is associated with T2D susceptibility, relative risk of obesity and insulin resistance, and lower indices of beta cell function. This common polymorphism is within a highly conserved region of the bioactive protein and leads to a single amino acid substitution (glycine 482 to serine).[review] PMID: 29186342
- We aimed to explore the potential therapeutic effect of PGC-1alpha by generating a lentiviral vector to express human PGC-1alpha and target it by stereotaxic delivery to hippocampus and cortex of APP23 transgenic mice . Four months after hPGC-1alpha injection, mice showed improved spatial and recognition memory concomitant with a significant reduction in Abeta deposition, associated with a decrease in BACE1 expression PMID: 27791018
- In this review, we discuss the interplay between PGC-1 coactivators and cancer pathogenesis, including tumor initiation, metastatic spread, and response to treatment. Given their involvement in the functional biology of cancers, identification of regulatory targets that influence PGC-1 expression and activity may reveal novel strategies suitable for mono- or combinatorial cancer therapies. [Review] PMID: 28607951
- Physiologic plasma FFA elevation in NGT individuals has opposing effects on PGC-1alpha non-CpG residue methylation (CpT demethylation and increased DNMT-3B expression), which is correlated with changes in PGC-1alpha expression and skeletal muscle mitochondrial function PMID: 29261667
- IFNgamma induced PPAR gamma coactivator-1 alpha (PGC-1alpha) positively regulated RIG-I; with PRMT-1 and G9a affecting PGC-1alpha in a counter-regulatory manner. PMID: 26725954
- These results show the importance of the activation of the MC1R-PGC-1alpha pathway for mitochondrial biogenesis and function in melanoma development, as well as BRAF for the antioxidant response regulated by PGC-1alpha. PMID: 28452072
- Postexercise expression of PGC-1alpha gene via the alternative promoter was not affected. PMID: 28074493
- The present study investigated the neuroprotective effects and signal transduction mechanisms of the overexpression of PGC-1alpha on N-methyl-4-phenylpyridinium ion (MPP(+))-induced mitochondrial damage in SH-SY5Y cell. PMID: 26141122
- These data indicate that acute exercise in a hot environment blunts expression of mitochondrial biogenesis-related mRNA, due to decreased binding of CREB, MEF2, and FoxO1 to the PGC-1alpha promoter. PMID: 27445305
- skeletal muscle PGC-1alpha is required for fasting-induced upregulation of skeletal muscle SIRT3 and maintaining high fat oxidation in the fasted state, but is dispensable for preserving the capability to switch substrate during the transition from the fed to the fasted state and for fasting-induced PDH regulation in skeletal muscle. PMID: 28400503
- Conversely, nitric oxide (NO) triggered post translation modifications of PGC-1alpha and induced FAO, recovering the bioenergetics impairment of muscles but shunting the defective mitochondrial biogenesis. PMID: 27974213
- The results revealed that the distribution of genotypes and allele frequency of the PGC-1alpha Gly482Ser polymorphism in polycystic ovary syndrome patients was statistically significant from those of the control group respectively indicating that the presence of 'A' allele might confer risk to polycystic ovary syndrome. PMID: 29030253
- mitochondrial biogenesis using mdivi1 or by silencing PGC1alpha abrogates differentiation of Glioblastoma multiforme differentiation into astroglia; conversely, overexpression of PGC1alpha elicits differentiation. PMID: 28076790
- PGC-1alpha protein was higher after HIHVT than after SIT (p < 0.05). Moreover, the AMPKpTHR172/AMPK ratio increased at post after SIT (p < 0.05), whereas this effect was delayed after HIHVT as it increased after 3 h PMID: 28973039
- study demonstrated that PGC1alpha serves as a unique adaptor molecule for the recruitment of additional coactivator proteins, which can finely regulate HBV (Hepatitis B Virus) transcription PMID: 28768874
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亞細胞定位:[Isoform 1]: Nucleus. Nucleus, PML body.; [Isoform B4]: Nucleus.; [Isoform B4-8a]: Cytoplasm. Nucleus.; [Isoform B5]: Nucleus. Nucleus, PML body.; [Isoform 9]: Nucleus.
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組織特異性:Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold i
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