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  4. Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP)

Recombinant Human Rap1 GTPase-activating protein 1 (RAP1GAP)

  • 中文名稱：
    人RAP1GAP重組蛋白
  • 貨號：
    CSB-EP019324HU
  • 規(guī)格：
    ￥1836
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 別名：
    (Rap1GAP)(Rap1GAP1)
  • 種屬：
    Homo sapiens (Human)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    80.3 kDa
  • 表達區(qū)域：
    1-663aa
  • 氨基酸序列
    MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNYYSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLVCEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESPAFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNKKPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQEVQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFSRSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGSSPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    GTPase activator for the nuclear Ras-related regulatory protein RAP-1A (KREV-1), converting it to the putatively inactive GDP-bound state.
  • 基因功能參考文獻：
    1. This is the first mechanistic study of Rap1Gap in breast cancer progression. Decreases in Rap1Gap expression led to changes in adherens junctions via reduction in E-cadherin levels, to cytoskeletal remodeling, and to increases in ERK activation that are correlated with an invasive phenotype in DCIS PMID: 30144784
    2. Rap1GAP may play a significant role in gastric cancer progression and act as a valuable prognostic marker for gastric cancer. PMID: 29758923
    3. results have shown a novel role of PLCepsilon in the maintenance of endothelial barrier function, via its CDC25 GEF domain and lipase activity, and subsequent up-regulation of Rap1 activity PMID: 27612188
    4. Findings suggest that RAP1 GTPase activating protein (Rap1GAP) is an important tumor suppressor with high prognostic value in endometrioid adenocarcinoma (EAC). PMID: 28196746
    5. Rap1GAP is ubiquitinated and degraded through proteasome pathway in mitosis. Rap1GAP interacts with PLK1 in vivo. PMID: 25329897
    6. kidney biopsies from glomerulosclerosis patients exhibited increased RAP1GAP, resulting in diminished glomerular RAP1 activation PMID: 24642466
    7. findings identify Rap1GAP as a critical regulator of aggressive tumor cell behavior and suggest that the level of Rap1GAP expression influences the migratory mechanisms that are operative in tumor cells. PMID: 23864657
    8. Results demonstrated that Rap1GAP promoted leukemia cell differentiation and apoptosis, but increased leukemia cell invasion in vitro. PMID: 22614916
    9. Downregulation of RAP1GAP in thyroid tumors enhances SRC-dependent signals that regulate cellular architecture and motility. PMID: 22696507
    10. Over-expression of Rap1GAP attenuated levels of both cadherins and integrins that are known to regulate the cancer cells invasion in renal cel carcinoma. PMID: 22266190
    11. Rap1GAP is a more effective inhibitor of cell-matrix adhesion compared to cell-cell adhesion. PMID: 21785277
    12. A polycomb-mediated repression of rap1GAP was demonstrated that involves EZH2, a histone methyltransferase in head and neck cancers. It was also shown that the loss of miR-101 expression correlates with EZH2 upregulation and rap1GAP downregulation. PMID: 21532618
    13. These studies establish that the direct physical interaction of Rap1 with KRIT1 enables the translocation of microtubule-sequestered KRIT1 to junctions, thereby supporting junctional integrity and cardiovascular development PMID: 21633110
    14. The expression level of RAP1GAp in myelodysplastic syndrome patients significantly increased as compared with patients with non-malignant blood diseases or AML. PMID: 19549374
    15. Ca2+-dependent monomer and dimer formation switches CAPRI Protein between Ras GTPase-activating protein (GAP) and RapGAP activities PMID: 21460216
    16. Results suggest that downregulation of Rap1GAP in epithelial tumors where alterations in cell/cell and cell/matrix adhesion are early steps in tumor dissemination supports a role for Rap1GAP depletion in tumor progression. PMID: 20439492
    17. Epigenetic or genetic loss of Rap1GAP is very common in thyroid cancer, where these events are sufficient to promote cell proliferation and invasion. PMID: 20124489
    18. Rap1GAP protein levels are tightly regulated and it may have a role as a tumor suppressor PMID: 14660640
    19. data identify rap1GAP as a putative tumor suppressor gene in pancreatic cancer PMID: 16424023
    20. Down-regulation of Rap1GAP is associated with oropharyngeal squamous cell carcinoma PMID: 16436672
    21. Data implicate Rap1GAP as a putative tumor/invasion suppressor in the thyroid, and show that downregulation of Rap1GAP contributes to Ras transformation. PMID: 17646383
    22. Rap1 increases KRIT-1 targeting to endothelial cell-cell junctions where it suppresses stress fibers and stabilizes junctional integrity. PMID: 17954608
    23. Rap1GAP inhibits tumor growth but induces MMP2- and MMP9-mediated SCC invasion and tumor progression, suggesting a role for this protein as a biomarker for early N-stage, aggressive SCCs PMID: 18483282
    24. Data propose that over-expression of RAP1GAP gene may play a role in the pathogenesis of myelodysplastic syndrome. PMID: 18551404
    25. a role for Rap1GAP depletion in the progression of human thyroid tumors, possibly through unrestrained Rap activity. PMID: 19066305
    26. Overexpression of Rap1GAP in melanoma cells blocks Rap1 activation and extracellular signal-regulated kinase (ERK) phosphorylation and inhibits melanoma cell proliferation and survival. PMID: 19147557
    27. model in which the Ras GAP-related domain of GAP1(IP4BP) functions to stabilize the switch II region of Rap1, allowing stabilization of the transition state during GTP hydrolysis initiated by the arginine finger PMID: 19433443

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  • 亞細胞定位：
    Golgi apparatus membrane; Peripheral membrane protein.
  • 組織特異性：
    Significant expression seen in the brain, kidney and pancreas. Abundant in the cerebral cortex and expressed at much lower levels in the spinal cord. Not detected in the lymphoid tissues.
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9858

    OMIM: 600278

    KEGG: hsa:5909

    STRING: 9606.ENSP00000290101

    UniGene: Hs.148178