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  4. Recombinant Human papillomavirus type 16 Protein E7 (E7)

Recombinant Human papillomavirus type 16 Protein E7 (E7)

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  • 中文名稱：
    重組人乳頭瘤病毒16型蛋白E7（E7）
  • 貨號(hào)：
    CSB-EP365855HML
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365855HML could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) E7.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365855HML could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) E7.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    E7; Protein E7
  • 種屬：
    Human papillomavirus type 16
  • 蛋白長(zhǎng)度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    15.0 kDa
  • 表達(dá)區(qū)域：
    1-98aa
  • 氨基酸序列
    MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQAEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Tris-based buffer,50% glycerol
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：
        重組人乳頭瘤病毒16型蛋白E7（E7），是乳頭瘤病毒（HPV）類型16的一個(gè)關(guān)鍵蛋白，與宿主細(xì)胞中的多個(gè)調(diào)控通路相互作用，被認(rèn)為在癌癥的發(fā)展中發(fā)揮著關(guān)鍵作用。HPV是一類常見的病毒，其中HPV-16型被廣泛認(rèn)為是引發(fā)宮頸癌等惡性疾病的主要致病毒株之一。E7蛋白是HPV感染中的一個(gè)關(guān)鍵因子，通過與宿主細(xì)胞的調(diào)控蛋白相互作用，影響細(xì)胞周期、凋亡和DNA修復(fù)等重要過程。因此，研究E7蛋白的結(jié)構(gòu)和功能對(duì)于理解HPV相關(guān)癌癥的發(fā)生機(jī)制至關(guān)重要。
        華美生物的重組人HPV16型E7蛋白是通過大腸桿菌系統(tǒng)表達(dá)的全長(zhǎng)蛋白，表達(dá)區(qū)域?yàn)?-98aa。該產(chǎn)品在體外實(shí)驗(yàn)、結(jié)合研究和功能實(shí)驗(yàn)中具有廣泛的應(yīng)用，為科研人員提供了一個(gè)工具，用于深入研究HPV感染和E7蛋白在宿主細(xì)胞中的作用機(jī)制。研究E7蛋白可以揭示其與宿主細(xì)胞分子通路的相互作用，有助于理解HPV相關(guān)疾病的發(fā)病機(jī)制，也為潛在的治療策略和疫苗開發(fā)提供了研究基礎(chǔ)。在癌癥生物學(xué)和病毒學(xué)領(lǐng)域，這一工具的應(yīng)用將有助于推動(dòng)我們對(duì)HPV感染相關(guān)疾病的深入認(rèn)識(shí)，為相關(guān)疾病的治療和預(yù)防提供新的思路。
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
  • 基因功能參考文獻(xiàn)：
    1. A role for E7 protein in regulation of Langerhans cells homeostasis in the skin and in suppression of antigen specific CD8 T cell expansion. PMID: 27708419
    2. E7 oncogene alters host gene expression in the cervical stroma. PMID: 29073104
    3. direct evidence that both A3A and HPV16 E7 interact with CUL2, suggesting that the E7-CUL2 complex formed during HPV infection may regulate A3A protein levels in the cell. PMID: 29367246
    4. Results suggest that E7 recruited CUL2, driven by CUL2/E2F1/miR-424 regulatory loop, is overexpressed and accelerates HPV16-induced cervical carcinogenesis. PMID: 27153550
    5. The human papillomavirus E7 oncoprotein is a regulator of host transcription. (Review) PMID: 27818212
    6. Findings indicate the interaction network of viral oncogene HPV16 E7, miR-27b and PLK2, and support the potential strategies using antisense nucleic acid of miR-27b for therapy of cervical cancer. PMID: 26910911
    7. Based on experimental data obtained from E7 proteins from the prototypical viral types 16, 18 and 11, we identified a couple of low pKa nucleophilic cysteines that can form a disulfide bridge upon the exposure to ROS and regulate the cytoplasm to nucleus transport PMID: 27863297
    8. E7 is major transforming oncoprotein of human papillomavirus 16 and serves as paradigmatic example of intrinsically disordered proteins due to its N-terminal disordered domain. Data suggest mutation of leucines in N-terminal intrinsically disordered domain of E7 leads to pronounced increase in both alpha-helix and beta-sheet structures; thus, E7 appears to exhibit local structural elements that oppose canonical folding. PMID: 28952717
    9. Authors observed thousands of unique HPV16 genomes; very few women shared the identical HPV16 sequence, however, E7 was devoid of variants in precancers/cancers compared to higher levels in the controls; this was confirmed in cancers from around the world. Strict conservation of the 98 amino acids of E7, which disrupts Rb function, is critical for HPV16 carcinogenesis. PMID: 28886384
    10. study determined that high-risk E7 proteins target the proteolysis of the cellular protein tyrosine phosphatase PTPN14 and find that this activity is correlated with the retinoblastoma-independent transforming activity of E7 PMID: 27651363
    11. Increased phosphorylation of the N29S of E7 increases interaction with TBP and pRb and viral transforming activity. PMID: 27829177
    12. HPV16 E7 oncoprotein upregulates Oct3/4, Sox2, Nanog and Fgf4 expression to maintain the self-renewal capacity of cancer stem cells. PMID: 27693927
    13. result indicates that cervical cancers do not require the continuous expression of E7 on the Fanconi anemia pathway-deficient background PMID: 27190216
    14. the level of Cdc6 phosphorylation at serine 54 (S54P) was increased in E7-expressing cells. S54P was associated with an increase in the total amount of Cdc6 and chromatin-bound Cdc6. DNA damage-enhanced upregulation and chromatin binding of Cdc6 appeared to be due to downregulation of cyclin-dependent kinase 1 (Cdk1) as Cdk1 knockdown increased Cdc6 levels PMID: 27207654
    15. PTPN14 is classified as a potential tumor suppressor protein, and is very susceptible to HPV E7-induced proteasome-mediated degradation. PMID: 28100625
    16. study found that HPV-16 decreased the phosphorylation of AKT (pAKT) and that this is a function of E7 that is independent of the Rb degradation function PMID: 27030265
    17. These data suggest that the HPV16 E7 oncoprotein impairs the function and morphology of dendritic cells and induces the systemic accumulation of CD11b(+)Gr1(+) cells. PMID: 27478837
    18. the structural features of the flexible N-terminal region (46 residues) of E7, were investigated. PMID: 27418281
    19. The human papillomavirus type 16 oncogene E7 may affect STK31 expression through a DNA methylation-mediated mechanism. PMID: 27044426
    20. HPV16 E7 protein can up-regulate host miR-21 expression, thus elevating cervical carcinoma cell growth, proliferation and invasion. PMID: 26884851
    21. These data suggest that local expression of HPV16-E7 in keratinocytes can contribute to persisting infection with this oncogenic virus, by altering the phenotype and function of local APCs. PMID: 27031095
    22. Results found that HPV16 E7 increases RARB mRNA and protein expression both in vitro and in the cervix of young K14E7 transgenic mice suggesting that RARB overexpression is part of the early molecular events induced by the E7 oncoprotein. PMID: 26173416
    23. E7 localised to the plasma membrane in cervical cancer cells. PMID: 26131956
    24. findings suggest that increased IL-17A expression by macrophages in E7-expressing skin exposed to DNCB promotes arginase-1 induction and contributes directly to the observed hyperinflammation. PMID: 25720383
    25. the modulation of HPV-16 E6/E7 expression remarkably influenced cell proliferation, migration, and invasion, as well as the protein levels of E-cadherin and P-cadherin in cervical cell lines. PMID: 26093522
    26. The data showed that E7 induced CCNA1 methylation by forming a complex with Dnmt1 at the CCNA1 promoter, resulting in the subsequent reduction of expression in cancers. PMID: 26250467
    27. In 10 microg/ml BV-treated CaSki cells, the mRNA expression and protein levels of HPV16 E7 were significantly decreased. The mRNA and protein expression levels of HPV16 E7 were decreased by bee venom in TC-1 tumors. PMID: 25633640
    28. that mast cells, recruited towards CCL2 and CCL5 expressed by epithelium induced to proliferate by Human Papillomavirus 16 E7 protein PMID: 25340820
    29. These results point to a mutually functional interaction between p14ARF and E7 that might partly explain why the sustained p14ARF expression observed in most cervical pre-malignant lesions and malignancies may be ineffective. PMID: 24798431
    30. In genetically engineered mouse models expressing HPV16 oncogenes in stratified squamous epithelia, HPV16 E7, alone or together with E6, led to an accumulation of epithelial cells harboring gamma-H2AX nuclear foci. PMID: 25216575
    31. Data indicate that HPV16E7, CBP/p300, and retinoblastoma protein pRb interactions are potentially important for cellular transformation. PMID: 25451029
    32. Our results demonstrate that HPV16.E7 protein enhances drug associated production of arginase-1 by myeloid cells and consequent inflammatory cellular infiltration of skin. PMID: 24732401
    33. The aim of the present study was to investigate the cytotoxicity of natural killer (NK) cells to CaSki cells following knockdown of the E7 protein of the human papillomavirus type 16. PMID: 24566606
    34. Results show that E7 interacts with the B-Myb, FoxM1 and LIN9 components of this activator complex, leading to cooperative transcriptional activation of mitotic genes in primary cells and E7 recruitment to the corresponding promoters. PMID: 24141769
    35. E7 induces cancer by causing DNA damage at least in part through the inactivation of pocket proteins. PMID: 24013229
    36. E7 induces human papillomavirus-associated head and neck cancers by promoting DNA damage through the inactivation of pocket proteins. PMID: 24086435
    37. HPV16 E7 oncoprotein increases production of the IL18BP in keratinocytes. PMID: 24478434
    38. The seven cysteines in HPV16 E7 remain reduced in conditions resembling the basal reduced state of a cell. PMID: 24559112
    39. This study demonstrates that the cdk inhibitor p16INK4A is required for high-level expression of the E7 oncoproteins of HPV-16, HPV-18, and HPV-45 in cervical carcinoma cells. PMID: 24599991
    40. These data suggest that the interaction of human papillomavirus E7 with p190 dysregulates this GTPase activating protein and alters the actin cytoskeleton. PMID: 24403595
    41. Matrix metalloproteinases (MMP)--MMP-1,-2,-9 and its endogenous activity regulators in transformed by E7 oncogene HPV16 and HPV18 cervical carcinoma cell lines PMID: 24479343
    42. E7 proteins of different types of human papillomaviruses disrupted pocket protein-DREAM complexes in a similar extent. PMID: 24294959
    43. hTERT cooperates with HPV16 E7 protein in mediating bypass of the senescence blockade and effecting cell immortalization PMID: 23592995
    44. Human papillomavirus type 16 E7 expression causes increased EMI1 mRNA expression and also inhibits EMI1 degradation. PMID: 24074588
    45. that a patch of hydrophobic residues, 65LRLCV69, within the zinc-binding domain of HPV16 E7 mediates its nuclear import via hydrophobic interactions with the FG domain of the central channel nucleoporin Nup62. PMID: 24074597
    46. HPV16-induced TLR9 down-regulation affects the interferon response which negatively regulates viral infection PMID: 23752229
    47. Authors investigated the effect of E7 on the late promoter and found that E7 was able to activate the promoter. PMID: 23725693
    48. expression of a single oncoprotein in the epidermis is sufficient for lymphocyte trafficking (including immunosuppressive lymphocytes) to premalignant skin PMID: 23469070
    49. Human papillomavirus 16 E7 Adenine647Guanine can be used as a candidate marker for the progression of the cervical neoplasia. PMID: 21535311
    50. These results demonstrate an important role for Cdt1 in human papillomavirus E7-induced rereplication and shed light on mechanisms by which human papillomavirus induces genomic instability. PMID: 23152514

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  • 亞細(xì)胞定位：
    Host cytoplasm. Host nucleus.
  • 蛋白家族：
    Papillomaviridae E7 protein family
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: vg:1489079