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Recombinant Macaca fascicularis Alpha-synuclein (SNCA)

In Stock
  • 中文名稱：
    獼猴SNCA重組蛋白
  • 貨號(hào)：
    CSB-YP021912MOV
  • 規(guī)格：
    ￥2616
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 別名：
    SNCA; Alpha-synuclein
  • 種屬：
    Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
  • 蛋白長(zhǎng)度：
    Full Length
  • 來(lái)源：
    Yeast
  • 分子量：
    16.5kDa
  • 表達(dá)區(qū)域：
    1-140aa
  • 氨基酸序列
    MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFIKKDQLGKNEEGAPQEGILQDMPVDPDNEAYEMPSEEGYQDYEPEA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標(biāo)簽：
    N-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    3-7 business days
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述：

    The DNA fragment encoding the 1-140aa of the Macaca fascicularis SNCA protein was fused with N-terminal 6xHis tag gene and then was inserted into the expression vector, which was subsequently transfected into the yeast cells for expression. The resulting product was further purified to obtain the recombinant Macaca fascicularis SNCA protein. The purity of this recombinant SNCA protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant SNCA protein showed a band on the gel with a molecular weight of approximately 17 kDa.

    In light of recent discoveries, researchers demonstrated that genetic triplication of the SNCA locus causes Parkinson disease. Another evidence discovered that the inhibition of SNCA enhances α‐synuclein in Parkinson's disease. SNCA is determined as one of susceptibility genes for Parkinson's disease. Albeit less is known about the mechanism of SNCA, SNCA is widely found to manipulate the mobility of synaptic vesicles. This regulation process is possible to disrupt the synaptic vesicle recycling and neurotransmitter release.

  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
  • 基因功能參考文獻(xiàn)：
    1. Study shows that the age-dependent alpha-syn accumulation is correlated with an elevation of TRPC3 in the mitochondrial fractions isolated from monkey and mouse brains. In animal and cell models, alpha-syn overexpression was accompanied by an elevation of alpha-syn and TRPC3 in the mitochondrial fractions, and alpha-syn downregulation was associated with a reduction of the mitochondrial alpha-syn and TRPC3. PMID: 27904950
  • 亞細(xì)胞定位：
    Cytoplasm. Membrane. Nucleus. Cell junction, synapse. Secreted.
  • 蛋白家族：
    Synuclein family
  • 數(shù)據(jù)庫(kù)鏈接：