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  4. Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-5 (Atf5)

Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-5 (Atf5)

Promotion
  • 中文名稱：
  • 貨號：
    CSB-EP002276MOa0
  • 規(guī)格：
    ￥2328
  • 促銷：
    現貨重組蛋白特價促銷
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產品詳情

  • 純度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    Atf5
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length
  • 來源：
    E.coli
  • 分子量：
    36.3 kDa
  • 表達區(qū)域：
    1-283aa
  • 氨基酸序列
    MSLLATLGLELDRALLPASGLGWLVDYGKLPLAPAPLGPYEVLGGALEGGLPGGGEPLAGDGFSDWMTERVDFTALLPLEAPLPPGTLPPPSPAPPDLEAMASLLKKELEQMEDFFLDAPLLPPPSPPPPPPPAAAPSLPLPLPLPTFDLPQPPTLDTLDLLAVYCRSEAGPGDSGLSTLPVPQQPPPLAPLPSPARPAPYPSPASTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELRERAESVEREIQYVKDLLIEVYKARSQRTRST
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    N-terminal 6xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    13-23 business days
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.
  • 基因功能參考文獻：
    1. Study reports that reduced levels of ATF5 in brain of Huntington's disease patients, probably due to its sequestration into the characteristic PolyQ containing neuronal inclusion bodies, correlates with decreased levels of the antiapoptotic protein MCL1, a transcriptional target of ATF5. Also provides evidence of decreased ATF5 being deleterious by rendering neurons more vulnerable to polyQ-induced apoptosis. PMID: 28861715
    2. Data indicate activating transcription factor 5 (ATF5) as a member of the transcriptional network governing pancreatic beta-cell survival during stress. PMID: 28115692
    3. ATF5 is one of the transcription factors crucial for the vomeronasal sensory formation. PMID: 26395637
    4. Atf5 is required for mouse olfactory bulb development via interneuron. PMID: 25704077
    5. Data indicate that downregulation of ATF5 inhibits adipogenesis through C/EBPalpha by impairing the interaction with p300-C/EBPbeta. PMID: 24216764
    6. Both ATF5 and CHOP have proapoptotic functions in mouse embryonic fibroblasts. PMID: 23761072
    7. Adult neurons express ATF5; its levels increase upon endoplasmic reticulum stress as a neuroprotective mechanism. PMID: 23518711
    8. ATF5 is required for terminal differentiation and survival of olfactory sensory neurons. PMID: 23090999
    9. BBF2H7-ATF5-MCL1 pathway specifically suppressed ER stress-induced apoptosis in chondrocytes. PMID: 22936798
    10. These findings indicate a reciprocal interaction between ATF5 and Shh in which Shh stimulates ATF5 expression and in which ATF5 contributes to Shh-stimulated cerebellar granule neuron progenitor cell expansion. PMID: 22095825
    11. Data show that transcription factor ATF5 is expressed in the postnatal brain. PMID: 21725368
    12. essential in malignant glioma genesis and ATF5-mediated survival pathway identified PMID: 20495567
    13. Inhibition of apoptosis by ATFx PMID: 12130540
    14. molecular cloning of two novel mRNAs, genomic organization, and odorant sensory neuron localization PMID: 12213205
    15. Data found that in Bmal1 -/- mice, the Atf5 expression could be regulated by CLOCK/brain and BMAL1 heterodimer by their binding to its E-box motif and repressing its promoter activity. PMID: 17823250
    16. ATF4 contributes to basal ATF5 transcription, and eIF2 kinases direct the translational expression of multiple transcription regulators by a mechanism involving delayed translation reinitiation PMID: 18195013
    17. The activating transcription factor 5 is a potent repressor of Tumor Suppressor Protein p53 and elevated expression of activating transcription factor 5 in a tumor. PMID: 19293535
    18. Fasting resulted in elevation of the expression of both Atf5 mRNA subtypes, Atf5-R1 and R2, in the liver. PMID: 19376136
    19. At the postnatal stage, Atf5 was expressed in epiphyseal chondrocytes and osteoblasts lining the bone trabeculae. PMID: 19712269

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    BZIP family
  • 組織特異性：
    Highly expressed in liver and at lower levels in heart, brain, lung, kidney, adipose tissue, and skeletal muscle. Expressed in some immature and in all mature olfactory sensory neurons (at protein level).
  • 數據庫鏈接：