Gdf5; Bmp14; Bp; Gdf-5Growth/differentiation factor 5; GDF-5; Bone morphogenetic protein 14; BMP-14
種屬:
Mus musculus (Mouse)
蛋白長(zhǎng)度:
Full Length of Mature Protein
來源:
E.coli
分子量:
17.6kDa
表達(dá)區(qū)域:
376-495aa
氨基酸序列
APLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白標(biāo)簽:
N-terminal 6xHis-tagged
產(chǎn)品提供形式:
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage state,
buffer ingredients, storage temperature and the stability of the protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction. Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG. Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8. Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes.
基因功能參考文獻(xiàn):
Microarray analysis of satellite cells showed that expression of growth differentiation factor 5 (Gdf5) mRNA was markedly increased in Dnmt3a-KO mice. The DNA methylation level of the Gdf5 promoter was markedly decreased in Dnmt3a-KO satellite cells. PMID: 29146735
spatiotemporal dynamics of Gdf5 expression may instruct lineage divergence. PMID: 27292641
he large array of modular enhancers for Gdf5 provide a new foundation for studying the spatial specificity of joint patterning in vertebrates, as well as new candidates for regulatory regions that may also influence osteoarthritis risk in human population PMID: 27902701
Growth differentiation factor 5 is a novel target-derived factor that promotes sympathetic axon growth and branching and makes a distinctive regional contribution to the establishment of sympathetic innervation. PMID: 26878848
Dach2 and Hdac9 mediate the effects of muscle activity on muscle reinnervation; Myog and Gdf5 appear to stimulate muscle reinnervation through parallel pathways PMID: 26483211
Clonal expansion of Gdf5 progenitors contributes to linear growth of the enthesis. PMID: 26141957
GDF5 might play a critical role in 3T3-L1 preadipocyte differentiation PMID: 25078108
These results suggest that PI3K/Akt signals play a role in the GDF5-mediated brown adipogenesis through a mechanism related to activation of the Smad pathway. PMID: 24944017
These results suggest that brown adipogenesis and energy homeostasis are both positively regulated by the GDF5/BMPR/Smad/PGC-1alpha signaling pathway in adipose tissues. PMID: 24062245
apical and basal dendritic arbours of pyramidal cells throughout the hippocampus were stunted in both homozygous and heterozygous Gdf5 null mutants, indicating that dendrite size and complexity are sensitive to the level of endogenous GDF5 synthesis. PMID: 24173804
This work implicates SOX11 as a potential regulator of GDF5 expression in joint maintenance and suggests a possible role in the pathogenesis of osteoarthritis PMID: 23356643
A novel molecular mechanism of a GDF5 mutation affecting chondrogenesis and osteogenesis, is reported. PMID: 21976273
Data show that revealed notochord cells in Gdf-5-null mice correctly form nuclei pulposi. PMID: 21278629
Although GDF-5 deficiency did not compromise long-term fracture healing, a delay in cartilage formation and remodeling supports roles for GDF-5 in the early phase of bone repair. PMID: 21590487
data suggest that decreased GDF5 levels in mice can contribute to osteoarthritis development by different mechanisms including altered loading and subchondral bone changes. PMID: 20805298
These observations indicate that GDF-5 regulates differentiation of both dental papilla and follicle during odontogenesis, co-operatively with other growth factors such as BMP-2. PMID: 19909214
GDF5 deficiency caused a 17% increase in medium diameter (100-225 nm) collagen fibrils in tail tendon, at the expense of larger fibrils. Thus, GDF5 may play a role in tendon homeostasis in mice. PMID: 11913489
GDF-5-deficient femora were weaker (-31%) and more compliant (-57%) than controls when tested to failure in torsion. PMID: 11996912
GDF-5 induced cell cycle arrest in the G1 phase before the appearance of apoptosis in mouse B cell hybridoma HS-72 cells PMID: 12464389
role of single and double mutations in the mouse Gdf6 and Gdf5 genes in multiple joint and skeletal patterning defects PMID: 12606286
GDF5 regulates expression of connexin 43 promoter in osteoblasts ans embryos PMID: 12881039
GDF-5 may play an important role in modulating tendon repair. Data are consistent with previously posited roles for GDF-5 in cell recruitment, migration/adhesion, differentiation, proliferation, and angiogenesis. PMID: 12919870
concordance between the mRNA expression profiles of GDF5 and the gap junction gene, Cx43, in the mouse embryonic limb, spine, and heart, consistent with coordinated functions for these gene products during developmental organogenesis PMID: 14613311
GDF-5 synergistically enhances de novo bone formation capability of bone marrow mesenchymal cells in hyaluronan composites in rats. PMID: 14661262
GDF5 has a role in growth of developing joints, including early joint interzones, adult articular cartilage, and the joint capsule PMID: 15492776
Excessive apoptosis in the absence of GDF5 results in developmental failure of the phalanges. PMID: 15542031
Deficiency in knockout mice affects biomechanical behavior and ultrastructure of mouse skin. PMID: 16112556
Results describe 2 mutations in growth and differentiation factor 5 (GDF5) that alter receptor-binding affinities. PMID: 16127465
Results suggest that CDMP1/GDF5 requires cleavage by two distinct proteolytic enzymes. PMID: 16829522
Study further highlights a critical role of GDF5 in joint formation and the development of osteoarthritis (OA), and this should serve as a good model for OA. PMID: 17656374
association of Gdf5-mediated signaling pathways with Trps1 and the phenotypic changes of ATDC5 cells due to over-expression or suppression of Trps1 PMID: 18363966
Absence of GDF5 does not interfere with lipopolysaccharides toll-like receptor signaling in a mouse model of arthritis. PMID: 19604444
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相關(guān)疾?。?/div>
Defects in Gdf5 are the cause of brachypodism (bp) which alters the length and numbers of bones in the limbs but spares the axial skeleton.