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Recombinant Mouse Netrin-1 (Ntn1)

  • 中文名稱：
    小鼠Ntn1重組蛋白
  • 貨號：
    CSB-MP016127MO
  • 規(guī)格：
    ￥1908
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長度：
    Full Length of Mature Protein
  • 來源：
    Mammalian cell
  • 分子量：
    66.9 kDa
  • 表達區(qū)域：
    25-604aa
  • 氨基酸序列
    GPGLSMFAGQAAQPDPCSDENGHPRRCIPDFVNAAFGKDVRVSSTCGRPPARYCVVSERGEERLRSCHLCNSSDPKKAHPPAFLTDLNNPHNLTCWQSENYLQFPHNVTLTLSLGKKFEVTYVSLQFCSPRPESMAIYKSMDYGRTWVPFQFYSTQCRKMYNRPHRAPITKQNEQEAVCTDSHTDMRPLSGGLIAFSTLDGRPSAHDFDNSPVLQDWVTATDIRVAFSRLHTFGDENEDDSELARDSYYYAVSDLQVGGRCKCNGHAARCVRDRDDSLVCDCRHNTAGPECDRCKPFHYDRPWQRATAREANECVACNCNLHARRCRFNMELYKLSGRKSGGVCLNCRHNTAGRHCHYCKEGFYRDMGKPITHRKACKACDCHPVGAAGKTCNQTTGQCPCKDGVTGITCNRCAKGYQQSRSPIAPCIKIPVAPPTTAASSVEEPEDCDSYCKASKGKLKMNMKKYCRKDYAVQIHILKADKAGDWWKFTVNIISVYKQGTSRIRRGDQSLWIRSRDIACKCPKIKPLKKYLLLGNAEDSPDQSGIVADKSSLVIQWRDTWARRLRKFQQREKKGKCKKA
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白標簽：
    C-terminal 10xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.
  • 基因功能參考文獻：
    1. potential effect of Ntn-1 on angiogenesis is further verified by the mouse hindlimb ischemia model, where the pre-activation of hUCB-MSCs with Ntn-1 significantly improved vascular regeneration. These results demonstrate that Ntn-1 plays an important role in the tissue regeneration process of hUCB-MSC via the lipid raft-mediated Inalpha6beta4 signaling pathway. PMID: 27881869
    2. Using a Ntn1 conditional knockout, we show that netrin 1, which is released at the pial surface by ventricular zone progenitors is responsible for the CNS confinement of precerebellar neurons. PMID: 29343638
    3. Collectively, our results defined netrin-1 as a positive regulator of malignant tumor metastasis in GC by activating the YAP signaling, with potential implications for new approaches to GC therapy. PMID: 29305865
    4. Data indicate that the formation of the hypothalamic NPY neural network is regulated at least partly by Netrin-1 signalling during normal in utero brain development in the mouse. PMID: 29121420
    5. Our findings suggest that netrin-1 can suppress the growth of PDAC and provide a mechanistic insight into this suppression. PMID: 27034160
    6. Diabetes decreases aortic levels of netrin-1. However, overexpression of netrin-1 attenuates diabetes-induced VED and limits the reduction of NO levels, while increasing expression of p-ERK1/2, and suppressing oxidative stress and inflammatory and apoptotic processes PMID: 29059224
    7. Ntn1 may play divergent cellular roles despite its conserved expression during canal morphogenesis in chicken and mouse. PMID: 28851705
    8. netrin1 provides "hederal" boundaries: a local growth substrate that promotes axon extension, while also preventing local innervation of netrin1-expressing domains. PMID: 28780049
    9. netrin-1 degradation products are capable of modulating vascular permeability in diabetic retinopathy PMID: 27400127
    10. Blockade of Netrin-1/Unc5b by monoclonal antibodies prevents bone destruction and reduces the severity of K/BxN serum transfer-induced arthritis PMID: 27502509
    11. Results suggest that Netrin1/DCC signaling induce neuronal migration in the dorsal spinal cord. PMID: 27784329
    12. results show that the classical view that attraction of commissural axons is mediated by a gradient of floor-plate-derived netrin-1 is inaccurate and that netrin-1 primarily acts locally by promoting growth cone adhesion. PMID: 28445456
    13. Here, we report that dissociation of UNC5C and polymerized TUBB3, the highly dynamic beta-tubulin isoform in neurons, is essential for netrin-1/UNC5C-promoted axon repulsion. These results not only provide a working model of direct modulation of MTs by guidance cues in growth cone navigation but also help us to understand molecular mechanisms underlying developmental brain disorders associated with TUBB3 mutations. PMID: 28483977
    14. Netrin1 does not act as a long-range secreted chemoattractant for commissural spinal axons but instead promotes ventrally directed axon outgrowth by haptotaxis, i.e., directed growth along an adhesive surface. PMID: 28434801
    15. Netrin-1(+/-) mice were protected against bleomycin-induced lung fibrosis and fibrocyte accumulation. PMID: 26749424
    16. netrin 1 treatment prevented bone erosion in an autoimmune arthritis model and age-related bone destruction. PMID: 27681594
    17. Netrin-1 reduced the Abeta1- 40-induced Abeta1-42 increase, increased sAbetaPPalpha, and reversed the Abeta-induced sAbetaPPalpha decrease in vitro; in vivo, induction of netrin-1 expression in PDAbetaPPSwe/Ind transgenic mice resulted in reductions in both Abeta1-42 and Abeta1- 40, and ICV delivery of netrin-1 to PDAbetaPPSwe/Ind mice increased sAbetaPPalpha, decreased Abeta, and improved working memory. PMID: 27060954
    18. The Netrin-1 and ephrin-B2 synergistic growth cone responses involve the potentiation of Src family kinase signaling, a common effector of both pathways. PMID: 26633881
    19. netrin1 prevented cardiac hypertrophy and heart failure through the negative regulation of the MEKK1-dependent MEKERK1/2 and JNK1/2 signaling pathways. PMID: 26783193
    20. The results of this study showed that NTN1 contributes to the formation of the Corpus callosum by promoting the preceding removal of the midline leptomeningeal cells and interhemispheric fusion. PMID: 26397040
    21. Akt-FoxO1 signaling pathway plays an essential role during liver regeneration PMID: 26573873
    22. netrin-1 loss-of-function causes embryonic lethality. PMID: 26257176
    23. Results show that the Netrin-1-induced axon slowdown is mediated by complex Ca2+ dynamics and involves Ca2+ efflux from the ER via the Ryanodine channels PMID: 26190161
    24. Netrin-1 haploinsufficiency leads to increased dopamine content in the medial prefrontal cortex. PMID: 26264903
    25. FLRT3 is a modulator of Netrin-1 response in developing axons. PMID: 24560577
    26. These results identify Netrin-1 as a key regulator of osteoclast differentiation that may be a new target for bone therapies. PMID: 25483983
    27. Low levels of Ntn1 can account for persistent attraction to the midline in hypomorphs. By contrast, Ntn1 null mice do not show all of the phenotypes reported for Unc5 receptor mutants, indicating that Ntn1 is not necessarily the dominant ligand for Unc5 family members in vivo and ruling out primary roles in survival or angiogenesis. PMID: 26395479
    28. Integration of shallow gradients of Shh and Netrin-1 synergistically guides commissural axons in the growing spinal cord. PMID: 25826604
    29. Results suggest that down syndrome cell adhesion molecule collaborates with deleted in colorectal cancer to regulate microtubule dynamics via direct binding to dynamic TUBB3 in Netrin-1-induced axon branching. PMID: 25754961
    30. Netrin-1 binds to CD146 with high affinity, inducing endothelial cell activation and downstream signaling in a CD146-dependent manner. PMID: 25656845
    31. Overall, these findings suggest that commissural axons use distinct mechanisms to navigate in different CNS regions. PMID: 26290247
    32. Small netrin-1-derived peptides are highly effective in protecting the heart against myocardial ischemia-reperfusion injury. PMID: 25924621
    33. Combination of netrin-1 and SIAH RNAi may prove to be a substantially effective therapy for myocardial infarction. PMID: 25561546
    34. Data indicate the roles of Slit1/Robo1 and Netrin1/DCC signals in positioning motor neuron cell bodies. PMID: 25530182
    35. This study reveal novel actions for Netrin-1 and provide evidence for a mechanism through which DA axons can selectively innervate one of two terminal zones in the striatum but have free reign to arborize widely within a terminal zone. PMID: 24867253
    36. These data demonstrate robust cardioprotective effect of netrin-1 in vivo, as shown by reduced infarct size and improved cardiac function mediated by netrin-1 receptor DCC. PMID: 24928309
    37. netrin-1 expression was simultaneously elevated with an increase of retinal neovascularization. PMID: 25149138
    38. This study show that Netrin1 initially attracts callosal pioneering axons derived from the cingulate cortex. PMID: 23302812
    39. netrin-1 and DCC are critical for the control of arterial innervation and blood flow regulation in peripheral organs. PMID: 24937433
    40. Results indicate that the vagus nerve regulates both netrin-1 and pro-resolving lipid mediators. PMID: 24863066
    41. Netrin-1 is expressed in the thymus by both thymic stromal cells and thymocytes. It can mediate cell adhesion and potentiate the chemotaxis for chemokine CXCL12. PMID: 24369474
    42. Netrin-1 has a rigid elongated structure containing two receptor-binding sites at opposite ends through which it brings together receptor molecules. PMID: 24876346
    43. novel model for the spatial regulation of axon branching by Netrin-1, in which localized plasma membrane expansion occurs via TRIM9-dependent regulation of SNARE-mediated vesicle fusion. PMID: 24778312
    44. Netrin-1 is highly expressed in obese but not lean adipose tissue, where it directs the retention of macrophages, promoting inflammation. NTN1 also promotes insulin resistance. PMID: 24584118
    45. Netrin 1/Dcc/Unc5 chemotropism contributes to axonal confinement within the central nervous system. PMID: 24449837
    46. Full-length and fragmented forms of netrin-1, found in multiple sclerosis lesions, have the capacity to inhibit oligodendrocyte precursor migration. PMID: 23831296
    47. These studies demonstrate a protective role for endogenous netrin-1 in attenuating diabetic kidney disease. PMID: 23636509
    48. a 1, 9 fold increase of Netrin-1 mRNA two weeks after nerve transection and repair in the nerve segment distal to the injury site. , Netrin-1 plays an important role during peripheral nerve regeneration. PMID: 23388207
    49. Model in which subventricular zone progenitors respond to a demyelination lesion by NTN1 secretion that both directly promotes cell emigration and contributes to local angiogenesis, which in turn facilitates progenitor cell emigration from the niche. PMID: 23824572
    50. Netrin-1 regulates colon-kidney cross talk through suppression of IL-6 function in a mouse model of colitis. PMID: 23445618

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  • 亞細胞定位：
    Secreted. Cytoplasm.
  • 組織特異性：
    In the embryo, widely expressed in the developing nervous system and in mesodermal tissues.
  • 數(shù)據(jù)庫鏈接：